Syphilis detection using the Siemens ADVIA Centaur Syphilis treponemal assay

Donkers, Bas; Levy, H. Roma; Vliet, Annemiek Letens-van

doi:10.1016/j.cca.2014.01.041

Cited by 18 publications

(17 citation statements)

References 10 publications

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“…The chemiluminescence and multiplex bead immunoassays are capable of high throughput and even random-access testing, but each is capable of running on the manufacturer's platform only. Limited data suggest that the performances of these assays for screening differ between testing platforms (32-34); however, reproducibility of results may be better achieved by the CIA (35).…”

Section: Serologic Testsmentioning

confidence: 99%

“…Reverse-screening algorithms allow automation and increased sample throughput but also address ergonomic issues for technologists and detect more cases of early as well as latent syphilis (35,47,48). These algorithms, employing fully automated systems, have been adopted by many private as well as public laboratories in Europe and North America.…”

Section: Diagnostic Algorithmsmentioning

confidence: 99%

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Recent Trends in the Serologic Diagnosis of Syphilis

Morshed

Singh

2014

Clin. Vaccine Immunol.

163

156

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bComplexities in the diagnosis of syphilis continue to challenge clinicians. While direct tests (e.g., microscopy or PCR) are helpful in early syphilis, the mainstay of diagnosis remains serologic tests. The traditional algorithm using a nontreponemal test (NTT) followed by a treponemal test (TT) remains the standard in many parts of the world. More recently, the ability to automate the TT has led to the increasingly widespread use of reverse algorithms using treponemal enzyme immunoassays (EIAs). Rapid, point-of-care TTs are in widespread use in developing countries because of low cost, ease of use, and reasonable performance. However, none of the current diagnostic algorithms are able to distinguish current from previously treated infections. In addition, the reversal of traditional syphilis algorithms has led to uncertainty in the clinical management of patients. The interpretation of syphilis tests is further complicated by the lack of a reliable gold standard for syphilis diagnostics, and the newer tests can result in false-positive reactions similar to those seen with older tests. Little progress has been made in the area of serologic diagnostics for congenital syphilis, which requires assessment of maternal treatment and serologic response as well as clinical and laboratory investigation of the neonate for appropriate management. The diagnosis of neurosyphilis continues to require the collection of cerebrospinal fluid for a combination of NTT and TT, and, while newer treponemal EIAs look promising, more studies are needed to confirm their utility. This article reviews current tests and discusses current controversies in syphilis diagnosis, with a focus on serologic tests.

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Section: Serologic Testsmentioning

confidence: 99%

Section: Diagnostic Algorithmsmentioning

confidence: 99%

Recent Trends in the Serologic Diagnosis of Syphilis

Morshed

Singh

2014

Clin. Vaccine Immunol.

163

156

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“…The HISCL anti-TP assay was also assessed for specificity in syphilis-negative samples and potentially interfering samples. As syphilis testing and testing algorithms vary from country to country, there is no gold standard for syphilis diagnosis (in fact, no definitive reference standard has been defined for syphilis testing) (12,17). A "true positive/truenegative" result was either clinically confirmed or defined as a sample positive/negative by at least two of the three treponemal assays used in the study.…”

Section: Figmentioning

confidence: 99%

“…In contrast, treponemal tests allow for the detection of specific treponemal antibodies and include the T. pallidum hemagglutination assay (TPHA), the T. pallidum particle agglutination assay (TPPA), the fluorescent treponemal antibody absorption (FTA-ABS) test, Western blot, enzyme immunoassay (EIA), and more recently the chemiluminescent immunoassay (CLIA) (9)(10)(11). Even though many diagnostic tests are available for syphilis, there is still no gold standard, and diagnosis usually relies upon a combination of tests (12). The traditional approach to the diagnosis of syphilis begins with a nontreponemal assay, either the VDRL test or, more commonly, the RPR test, which detects anticardiolipin antibodies (13).…”

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confidence: 99%

Evaluation of the HISCL Anti-Treponema pallidum Assay as a Screening Test for Syphilis

Chen

Liu

et al. 2015

Clin Vaccine Immunol

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The resurgence of syphilis in recent years has become a serious threat to public health worldwide, and the serological detection of specific antibodies against Treponema pallidum remains the most reliable method for laboratory diagnosis of syphilis. This study examined the performance of the recently launched HISCL anti-Treponema pallidum (anti-TP) assay as a screening test for syphilis in a high-volume laboratory. The HISCL anti-TP assay was tested in 300 preselected syphilis-positive samples, 704 fresh syphilis-negative samples, 48 preselected potentially interfering samples, and 30 "borderline" samples and was compared head to head with the commercially available Lumipulse G TP-N. In this study, the HISCL anti-TP assay was in perfect agreement with the applied testing algorithms with an overall agreement of 100%, comparable to that of Lumipulse G TP-N (99.63%). The sensitivity and specificity of the HISCL anti-TP assay were 100% (95% confidence interval [CI], 98.42% to 100%) and 100% (95% CI, 99.37% to 100%), respectively. Considering the excellent ease of use and automation, high throughput, and its favorable sensitivity and specificity, the HISCL anti-TP assay may represent a new choice for syphilis screening in high-volume laboratories.

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“…This approach is the most suitable for those laboratories with a huge workload. Treponemal tests are available in the automated format, and had already proved their high efficiency for syphilis screening (Donkers et al, 2014). Similarly, the availability of a sensitive and specific automated non-treponemal test can help in improving syphilis testing.…”

Section: Role Of Automated Duplex Test In Improving Syphilis Testingmentioning

confidence: 99%

Can a Duplex Non-treponemal and Treponemal Serological Test Help in Improving Syphilis Testing?

Shukla¹

2016

Int.J.Curr.Microbiol.App.Sci

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Syphilis detection using the Siemens ADVIA Centaur Syphilis treponemal assay

Abstract: The Centaur Syphilis assay performance is comparable to the other 2 commercial assays.

Cited by 18 publications

References 10 publications

Recent Trends in the Serologic Diagnosis of Syphilis

Recent Trends in the Serologic Diagnosis of Syphilis

Evaluation of the HISCL Anti-Treponema pallidum Assay as a Screening Test for Syphilis

Can a Duplex Non-treponemal and Treponemal Serological Test Help in Improving Syphilis Testing?

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