Syntrophomonas palmitatica sp. nov., an anaerobic, syntrophic, long-chain fatty-acid-oxidizing bacterium isolated from methanogenic sludge

Hatamoto, Masashi; Imachi, Hiroyuki; Fukayo, Sanae; Ohashi, Akiyoshi; Harada, Hideki

doi:10.1099/ijs.0.64981-0

Cited by 90 publications

(41 citation statements)

References 33 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The degradation of LCFA (19,20), aromatics (12,47), and alkanes (1, 51) has been observed in methanogenic consortia. The degradation of these compounds in the absence of a terminal electron acceptor (TEA) for respiration involves the syntrophic cooperation of microorganisms; i.e., the reactions are thermodynamically driven by the removal of H 2 (32) and result in accumulation of acetate and propionate (Fig.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Stimulation of Methane Generation from Nonproductive Coal by Addition of Nutrients or a Microbial Consortium

Jones

Voytek

Corum

et al. 2010

Appl Environ Microbiol

230

142

View full text Add to dashboard Cite

Biogenic formation of methane from coal is of great interest as an underexploited source of clean energy. The goal of some coal bed producers is to extend coal bed methane productivity and to utilize hydrocarbon wastes such as coal slurry to generate new methane. However, the process and factors controlling the process, and thus ways to stimulate it, are poorly understood. Subbituminous coal from a nonproductive well in south Texas was stimulated to produce methane in microcosms when the native population was supplemented with nutrients (biostimulation) or when nutrients and a consortium of bacteria and methanogens enriched from wetland sediment were added (bioaugmentation). The native population enriched by nutrient addition included Pseudomonas spp., Veillonellaceae, and Methanosarcina barkeri. The bioaugmented microcosm generated methane more rapidly and to a higher concentration than the biostimulated microcosm. Dissolved organics, including long-chain fatty acids, single-ring aromatics, and long-chain alkanes accumulated in the first 39 days of the bioaugmented microcosm and were then degraded, accompanied by generation of methane. The bioaugmented microcosm was dominated by Geobacter sp., and most of the methane generation was associated with growth of Methanosaeta concilii. The ability of the bioaugmentation culture to produce methane from coal intermediates was confirmed in incubations of culture with representative organic compounds. This study indicates that methane production could be stimulated at the nonproductive field site and that low microbial biomass may be limiting in situ methane generation. In addition, the microcosm study suggests that the pathway for generating methane from coal involves complex microbial partnerships.

show abstract

mentioning

confidence: 99%

“…6. Proposed model of biogenic methane generation from coal based on the current study and previous work in other laboratories (9,11,12,15,19,20,37,51). H 2 may be removed by methanogenesis, acetogenesis, or by a bacterial partner with a TEA.…”

mentioning

confidence: 99%

Stimulation of Methane Generation from Nonproductive Coal by Addition of Nutrients or a Microbial Consortium

Jones

Voytek

Corum

et al. 2010

Appl Environ Microbiol

230

142

View full text Add to dashboard Cite

show abstract

“…Due to the syntrophic metabolism and toxicity of LCFA, isolation of LCFA-degrading syntrophs is difficult. Thus, information on LCFA-degrading bacteria in pure culture is based on Syntrophomonas species (10,21,36,47,54) and on Thermosyntropha lipolytica (49) of the family Syntrophomonadaceae. Syntrophus aciditrophicus (15) is also an LCFA degrader; however, this strain is more often identified as a benzoate degrader.…”

mentioning

confidence: 99%

Diversity of Anaerobic Microorganisms Involved in Long-Chain Fatty Acid Degradation in Methanogenic Sludges as Revealed by RNA-Based Stable Isotope Probing

Hatamoto

Imachi

Yashiro

et al. 2007

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Long-chain fatty acid (LCFA) degradation is a key step in methanogenic treatment of wastes/wastewaters containing high concentrations of lipids. However, despite the importance of LCFA-degrading bacteria, their natural diversity is little explored due to the limited availability of isolate information and the lack of appropriate molecular markers. We therefore investigated these microbes by using RNA-based stable isotope probing. We incubated four methanogenic sludges (mesophilic sludges MP and MBF and thermophilic sludges TP and JET) with 13 C-labeled palmitate (1 mM) as a substrate. After 8 to 19 days of incubation, we could detect 13 C-labeled bacterial rRNA. A density-resolved terminal restriction fragment length polymorphism fingerprinting analysis showed distinct bacterial populations in 13 C-labeled and unlabeled rRNA fractions. The bacterial populations in the 13 C-labeled rRNA fractions were identified by cloning and sequencing of reversetranscribed 16S rRNA. Diverse phylogenetic bacterial sequences were retrieved, including those of members of the family Syntrophaceae, clone cluster MST belonging to the class Deltaproteobacteria, Clostridium clusters III and IV, phylum Bacteroidetes, phylum Spirochaetes, and family Syntrophomonadaceae. Although Syntrophomonadaceae species are considered to be the major fatty acid-degrading syntrophic microorganisms under methanogenic conditions, they were detected in only two of the clone libraries. These results suggest that phylogenetically diverse bacterial groups were active in situ in the degradation of LCFA under methanogenic conditions.Lipid is a one of the major organic fractions of wastes/ wastewaters, and lipid-rich wastes/wastewaters are widely found in certain food processing industries, such as dairy, edible oil, and slaughterhouses (20). Because lipids have a high theoretical methane yield in comparison with other organic substances, methanogenic treatment has been applied to lipidrich wastes/wastewaters but resulted in low organic loading rates (see, for example, references 16 and 50) compared to that seen for other types of wastes/wastewaters. This is at least partly due to the acute toxicity of long-chain fatty acids (LCFA), which are the main constituent and hydrolysate of lipids in the anaerobic consortium. LCFA can cause substrate toxicity in anaerobic microorganisms (see, for example, references 18 and 44) and tend to adsorb onto the biomass and flow out of the reactor.Under methanogenic conditions, LCFA degradation requires a syntrophic association of LCFA-degrading anaerobes and hydrogenotrophic methanogens, because the oxidation of LCFA is thermodynamically unfavorable in such environments unless the consumption of reducing equivalents (hydrogen and/or formate) is coupled with oxidation (37). Due to the syntrophic metabolism and toxicity of LCFA, isolation of LCFA-degrading syntrophs is difficult. Thus, information on LCFA-degrading bacteria in pure culture is based on Syntrophomonas species (10, 21, 36, 47, 54) and on Thermosyntropha lipolytica...

show abstract

“…Bacteria of the genus Syntrophomonas were shown to take part in the degradation of LCFA in which they live in close syntrophic relation to hydrogenotrophic methanogens such as Methanobacterium formicicum (Sousa et al, 2007b) or Methanospirillum hungatei (Hatamoto et al, 2007). As the hydrogen partial pressure was too high for propionic acid degradation, presumably, Syntrophomonas was at an advantage compared to other bacteria due to the hydrogen consumption of its syntrophic partner…”

Section: Discussionmentioning

confidence: 99%

Application of an early warning indicator and CaO to maximize the time–space-yield of an completely mixed waste digester using rape seed oil as co-substrate

et al. 2014

View full text Add to dashboard Cite

Originally published as:Kleyböcker, A., Lienen, T., Liebrich, M., Kasina, M., Kraume, M., Würdemann, H. (2014): Application of an early warning indicator and CaO to maximize the time-space-yield of a completely mixed waste digester using rape seed oil as co-substrate. AbstractIn order to increase the organic loading rate (OLR) and hereby the performance of biogas plants an early warning indicator (EWI-VFA/Ca) was applied in a laboratory-scale biogas digester to control process stability and to steer additive dosing. As soon as the EWI-VFA/Ca indicated the change from stable to instable process conditions, calcium oxide was charged as a countermeasure to raise the pH and to bind long-chain fatty acids (LCFAs) by formation ofaggregates. An interval of eight days between two increases of the OLR, which corresponded to 38% of one hydraulic residence time (HRT), was sufficient for process adaptation. An OLR increase by a factor of three within six weeks was successfully used for biogas production. The OLR was increased to 9.5 kg volatile solids (VS) m -3 d -1 with up to 87% of fat. The high loading rates affected neither the microbial community negatively nor the biogas production process.Despite the increase of the organic load to high rates, methane production yielded almost its optimum, amounting to 0.9 m³ (kg VS)

show abstract

Syntrophomonas palmitatica sp. nov., an anaerobic, syntrophic, long-chain fatty-acid-oxidizing bacterium isolated from methanogenic sludge

Cited by 90 publications

References 33 publications

Stimulation of Methane Generation from Nonproductive Coal by Addition of Nutrients or a Microbial Consortium

Stimulation of Methane Generation from Nonproductive Coal by Addition of Nutrients or a Microbial Consortium

Diversity of Anaerobic Microorganisms Involved in Long-Chain Fatty Acid Degradation in Methanogenic Sludges as Revealed by RNA-Based Stable Isotope Probing

Application of an early warning indicator and CaO to maximize the time–space-yield of an completely mixed waste digester using rape seed oil as co-substrate

Contact Info

Product

Resources

About