Syntrophic metabolism of a co-culture containing Clostridium cellulolyticum and Rhodopseudomonas palustris for hydrogen production

Jiao, Yongqin; Navid, Ali; Stewart, Benjamin; McKinlay, James B.; Thelen, Michael P.; Pett‐Ridge, Jennifer

doi:10.1016/j.ijhydene.2012.05.100

Cited by 35 publications

(28 citation statements)

References 30 publications

(20 reference statements)

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“…Cocultures pairing fermentative bacteria with purple photoheterotrophic bacteria have been of interest for over 30 years (Odom and Wall, 1983;Fang et al, 2006;Ding et al, 2009;Sun et al, 2010;Jiao et al, 2012), primarily as a consolidated bioprocess for converting plant-derived sugars into H 2 . However, this combination of bacteria also resembles a natural anaerobic food web; fermentative bacteria consume plant-derived sugars and excrete products that serve as a carbon source for photoheterotrophs ( Figure 1a).…”

Section: Introductionmentioning

confidence: 99%

Microbial mutualism dynamics governed by dose-dependent toxicity of cross-fed nutrients

et al. 2016

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Microbial interactions, including mutualistic nutrient exchange (cross-feeding), underpin the flow of energy and materials in all ecosystems. Metabolic exchanges are difficult to assess within natural systems. As such, the impact of exchange levels on ecosystem dynamics and function remains unclear. To assess how cross-feeding levels govern mutualism behavior, we developed a bacterial coculture amenable to both modeling and experimental manipulation. In this coculture, which resembles an anaerobic food web, fermentative Escherichia coli and photoheterotrophic Rhodopseudomonas palustris obligately cross-feed carbon (organic acids) and nitrogen (ammonium). This reciprocal exchange enforced immediate stable coexistence and coupled species growth. Genetic engineering of R. palustris to increase ammonium cross-feeding elicited increased reciprocal organic acid production from E. coli, resulting in culture acidification. Consequently, organic acid function shifted from that of a nutrient to an inhibitor, ultimately biasing species ratios and decreasing carbon transformation efficiency by the community; nonetheless, stable coexistence persisted at a new equilibrium. Thus, disrupting the symmetry of nutrient exchange can amplify alternative roles of an exchanged resource and thereby alter community function. These results have implications for our understanding of mutualistic interactions and the use of microbial consortia as biotechnology.

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Section: Introductionmentioning

confidence: 99%

Microbial mutualism dynamics governed by dose-dependent toxicity of cross-fed nutrients

et al. 2016

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“…In summary, a number of physiological studies of H 2 -producing cocultures (18)(19)(20)(21)26) incorporating dark-fermentative and photosynthetic bacteria have shown that H 2 yield and substrate utilization are enhanced during coculturing. However, the detailed molecular mechanisms of how each organism in the coculture responds to the presence of the culturing partner and the changing culturing conditions (e.g., pH) remain unclear.…”

Section: Resultsmentioning

confidence: 99%

“…In the field of biofuels, such as biohydrogen, coculture models have also been established. For example, studies have demonstrated that cocultures containing dark-fermentative bacteria such as Clostridium species and photosynthetic bacteria such as Rhodopseudomonas species can enhance hydrogen (H 2 ) production and substrate consumption (16)(17)(18)(19)(20)(21)(22).…”

mentioning

confidence: 99%

“…While H 2 production from dark-fermentative and photosynthetic bacteria in cocultures has been intensively investigated in small-scale experiments and large-scale reactors (8,10,19,21,(25)(26)(27), the molecular mechanisms governing the bacterial dynamics and interactions remain unclear. In particular, to the best of our knowledge, the use of a high-throughput sequencing (RNA-seq) strategy to query the transcriptomic responses of H 2 -producing cocultures utilizing cellulose as the sole carbon source has not been previously investigated.…”

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confidence: 99%

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Transcriptomic Responses of the Interactions between Clostridium cellulovorans 743B and Rhodopseudomonas palustris CGA009 in a Cellulose-Grown Coculture for Enhanced Hydrogen Production

Chen

Jia

et al. 2016

Appl Environ Microbiol

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Coculturing dark-and photofermentative bacteria is a promising strategy for enhanced hydrogen (H 2 ) production. In this study, next-generation sequencing was used to query the global transcriptomic responses of an artificial coculture of Clostridium cellulovorans 743B and Rhodopseudomonas palustris CGA009. By analyzing differentially regulated gene expression, we showed that, consistent with the physiological observations of enhanced H 2 production and cellulose degradation, the nitrogen fixation genes in R. palustris and the cellulosomal genes in C. cellulovorans were upregulated in cocultures. Unexpectedly, genes related to H 2 production in C. cellulovorans were downregulated, suggesting that the enhanced H 2 yield was contributed mainly by R. palustris. A number of genes related to biosynthesis of volatile fatty acids (VFAs) in C. cellulovorans were upregulated, and correspondingly, a gene that mediates organic compound catabolism in R. palustris was also upregulated. Interestingly, a number of genes responsible for chemotaxis in R. palustris were upregulated, which might be elicited by the VFA concentration gradient created by C. cellulovorans. In addition, genes responsible for sulfur and thiamine metabolism in C. cellulovorans were downregulated in cocultures, and this could be due to a response to pH changes. A conceptual model illustrating the interactions between the two organisms was constructed based on the transcriptomic results. IMPORTANCEThe findings of this study have important biotechnology applications for biohydrogen production using renewable cellulose, which is an industrially and economically important bioenergy process. Since the molecular characteristics of the interactions of a coculture when cellulose is the substrate are still unclear, this work will be of interest to microbiologists seeking to better understand and optimize hydrogen-producing coculture systems. Microorganisms do not live in isolation in nature; instead, they interact with each other in complex ecological networks within a microbial community in order to function and resist stresses in various environments (1). In general, interspecies interactions define the characteristics and robustness of microbial communities. The concept of microbe-microbe interactions has been harnessed and applied in the bioremediation (2, 3), food and beverage (4-7), and biofuel (8-12) industries. The application of multispecies systems could be a promising alternative bioprocess strategy to the use of single species, which requires extensive genetic engineering before multiple desirable traits are incorporated, whereas these functions are distributed among different organisms in a multispecies system (13). However, multispecies systems can be challenging to control (14); therefore, a detailed understanding of the physiological and molecular mechanisms of microbial interactions is important in engineering robust complex microbial communities.Studying the interactions that occur in a complex microbial community involving hundreds of sp...

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“…As espécies mais reportadas e estudadas são C. acetobutylicum (Cappelletti et al 2011). C. butyricum (Wang & Jin, 2009); C. beijerinckii (Ye et al 2012); C. thermocellum (Ellis et al 2012); e Clostridium cellulolyticum (Jiao et al 2012). Entretanto, neste trabalho, a abundância relativa desse gênero foi baixa (10° e 30° dia de operação do reator com polietileno de baixa densidade, 12,8% e 5,3%, respectivamente).…”

Section: Avaliação Dos Produtos Intermediáriosunclassified

Digestão anaeróbia da vinhaça da cana de açúcar em reator acidogênico de leito fixo seguido de reator metanogênico de manta de lodo

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Syntrophic metabolism of a co-culture containing Clostridium cellulolyticum and Rhodopseudomonas palustris for hydrogen production

Cited by 35 publications

References 30 publications

Microbial mutualism dynamics governed by dose-dependent toxicity of cross-fed nutrients

Microbial mutualism dynamics governed by dose-dependent toxicity of cross-fed nutrients

Transcriptomic Responses of the Interactions between Clostridium cellulovorans 743B and Rhodopseudomonas palustris CGA009 in a Cellulose-Grown Coculture for Enhanced Hydrogen Production

Digestão anaeróbia da vinhaça da cana de açúcar em reator acidogênico de leito fixo seguido de reator metanogênico de manta de lodo

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