Syntrophic Acetate-Oxidizing Microbes in Methanogenic Environments

Hattori, Satoshi

doi:10.1264/jsme2.23.118

Cited by 400 publications

(271 citation statements)

References 91 publications

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“…Consequently, methane production was high in the enrichments incubated at 55 and 65 1C (Figures 4a and b). The dynamics of H 2 and methane consumption/production in these enrichments were obviously similar to earlier observations in syntrophic co-cultures of fermentative bacteria and hydrogenotrophic methanogens (Hattori, 2008). These results strongly suggest that anaerobic biodegradation supports methanogenesis through a coupled mechanism in which H 2 released from the anaerobic degradation of organic matter is taken up in the transformation of CO 2 to methane, and that these biochemical steps take place simultaneously.…”

Section: Discussionsupporting

confidence: 87%

Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan

et al. 2009

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To identify the methanogenic pathways present in a deep aquifer associated with an accretionary prism in Southwest Japan, a series of geochemical and microbiological studies of natural gas and groundwater derived from a deep aquifer were performed. Stable carbon isotopic analysis of methane in the natural gas and dissolved inorganic carbon (mainly bicarbonate) in groundwater suggested that the methane was derived from both thermogenic and biogenic processes. Archaeal 16S rRNA gene analysis revealed the dominance of H 2 -using methanogens in the groundwater. Furthermore, the high potential of methane production by H 2 -using methanogens was shown in enrichments using groundwater amended with H 2 and CO 2 . Bacterial 16S rRNA gene analysis showed that fermentative bacteria inhabited the deep aquifer. Anaerobic incubations using groundwater amended with organic substrates and bromoethanesulfonate (a methanogen inhibitor) suggested a high potential of H 2 and CO 2 generation by fermentative bacteria. To confirm whether or not methane is produced by a syntrophic consortium of H 2 -producing fermentative bacteria and H 2 -using methanogens, anaerobic incubations using the groundwater amended with organic substrates were performed. Consequently, H 2 accumulation and rapid methane production were observed in these enrichments incubated at 55 and 65 1C. Thus, our results suggested that past and ongoing syntrophic biodegradation of organic compounds by H 2 -producing fermentative bacteria and H 2 -using methanogens, as well as a thermogenic reaction, contributes to the significant methane reserves in the deep aquifer associated with the accretionary prism in Southwest Japan.

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Section: Discussionsupporting

confidence: 87%

Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan

et al. 2009

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“…A syntrophic relationship with sulfate-reducing bacteria (SRB) is often observed under sufficient sulfate concentrations ( Fig. 1B; Imachi et al 2006 and2008). The second cluster (ca.…”

Section: Depth Distribution Of Methanogenic Speciesmentioning

confidence: 92%

Influence of Methanogenic Populations in Holocene Lacustrine Sediments Revealed by Clone Libraries and Fatty Acid Biogeochemistry

Vuillemin

Ariztegui

Nobbe

et al. 2014

Geomicrobiology Journal

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Influence of methanogenic populations in Holocene lacustrine sediments revealed by clone libraries and fatty acid biogeochemistry Abstract Methanogenic populations were investigated in subsaline Laguna Potrok Aike sediments, southern Argentina. Microbial density and activity were assessed via cell count and in situ ATP detection for the last ~11K years. Methanogen phylogenetics highlighted species stratification throughout depth, whereas CO 2 reduction was the major pathway leading to methane production. Organic substrates, characterized using pore water analysis, bulk organic fractions and saturated fatty acids, showed a clear link between sediment colonization and initial organic sources. Concentrations and 13 C compositions of methane and fatty acids provided final evidence of a microbial imprint on Holocene organic proxies in the most colonized intervals.

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“…This transition from hydrogenotrophic methanogenesis to aceticlastic methanogenesis is supported by several lines of evidence; (i) acetate concentrations in effluent seawater gradually decreased in the late stages of reactor operation (Table 1), (ii) the clonal frequency of the aceticlastic methanogen Methanosarcina increased with operational time (Figure 2) and (iii) an acetate-degrading enrichment culture containing a member of Methanosarcina was obtained by batch culture (AYEAV10 culture in Supplementary Table S8). On the other hand, acetate degradation can also occur via the syntrophic association of acetatedegrading bacteria and hydrogenotrophic methanogens (Hattori, 2008). In our DHS experiment, syntrophic acetate oxidation likely occurred because an acetate-degrading culture (Ace25) did not contain aceticlastic methanogen groups such as Methanosarcina but consisted of a hydrogenotrophic methanogen Methanoculleus species and several bacterial species (Supplementary Table S8).…”

mentioning

confidence: 92%

Cultivation of methanogenic community from subseafloor sediments using a continuous-flow bioreactor

et al. 2011

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Microbial methanogenesis in subseafloor sediments is a key process in the carbon cycle on the Earth. However, the cultivation-dependent evidences have been poorly demonstrated. Here we report the cultivation of a methanogenic microbial consortium from subseafloor sediments using a continuous-flow-type bioreactor with polyurethane sponges as microbial habitats, called down-flow hanging sponge (DHS) reactor. We anaerobically incubated methane-rich core sediments collected from off Shimokita Peninsula, Japan, for 826 days in the reactor at 10 1C. Synthetic seawater supplemented with glucose, yeast extract, acetate and propionate as potential energy sources was provided into the reactor. After 289 days of operation, microbiological methane production became evident. Fluorescence in situ hybridization analysis revealed the presence of metabolically active microbial cells with various morphologies in the reactor. DNA-and RNA-based phylogenetic analyses targeting 16S rRNA indicated the successful growth of phylogenetically diverse microbial components during cultivation in the reactor. Most of the phylotypes in the reactor, once it made methane, were more closely related to culture sequences than to the subsurface environmental sequence. Potentially methanogenic phylotypes related to the genera Methanobacterium, Methanococcoides and Methanosarcina were predominantly detected concomitantly with methane production, while uncultured archaeal phylotypes were also detected. Using the methanogenic community enrichment as subsequent inocula, traditional batch-type cultivations led to the successful isolation of several anaerobic microbes including those methanogens. Our results substantiate that the DHS bioreactor is a useful system for the enrichment of numerous fastidious microbes from subseafloor sediments and will enable the physiological and ecological characterization of pure cultures of previously uncultivated subseafloor microbial life.

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Syntrophic Acetate-Oxidizing Microbes in Methanogenic Environments

Cited by 400 publications

References 91 publications

Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan

Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan

Influence of Methanogenic Populations in Holocene Lacustrine Sediments Revealed by Clone Libraries and Fatty Acid Biogeochemistry

Cultivation of methanogenic community from subseafloor sediments using a continuous-flow bioreactor

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