Syntrophic acetate oxidation replaces acetoclastic methanogenesis during thermophilic digestion of biowaste

Dyksma, Stefan; Jansen, Lukas; Gallert, Claudia

doi:10.1186/s40168-020-00862-5

Cited by 156 publications

(72 citation statements)

References 86 publications

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“…Firmicutes and Bacteroidetes are known to be involved in the hydrolytic degradation of cellulosic compounds. Hence, they were found to be dominant in agricultural-based biogas plants or biogas fermenters fed with cellulosic compounds [ 55 , 56 , 58 , 65 , 66 , 67 , 68 , 69 ]. Both phyla, Firmicutes and Bacteroidetes , can be exchanged with each other, as shown by [ 57 ], in a long-term fermentation period of 1750 days, with fodder beet silage as a mono-substrate.…”

Section: Resultsmentioning

confidence: 99%

“…However, Mollicutes were also recognized in the mesophilic fermenter F1, F3, and F4 ( Figure 6 ). Furthermore, Mollicutes have been observed in some agricultural-based biogas plants [ 58 ] and a thermophilic biowaste digester [ 56 ]. The clostridial Defluviitalea belongs to the Mollicutes and was found to be present here, with 4–14% as the sole thermophilic genus of this group with an abundance >3% (bright green, Figure 7 A).…”

Section: Resultsmentioning

confidence: 99%

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Mesophilic and Thermophilic Anaerobic Digestion of Wheat Straw in a CSTR System with ‘Synthetic Manure’: Impact of Nickel and Tungsten on Methane Yields, Cell Count, and Microbiome

et al. 2022

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Lignocellulosic residues, such as straw, are currently considered as candidates for biogas production. Therefore, straw fermentations were performed to quantitatively estimate methane yields and cell counts, as well as to qualitatively determine the microbiome. Six fully automated, continuously stirred biogas reactors were used: three mesophilic (41 °C) and three thermophilic (58 °C). They were fed every 8 h with milled wheat straw suspension in a defined, buffered salt solution, called ‘synthetic manure’. Total reflection X-ray fluorescence spectrometry analyses showed nickel and tungsten deficiency in the straw suspension. Supplementation of nickel and subsequently tungsten, or with an increasing combined dosage of both elements, resulted in a final concentration of approximately 0.1 mg/L active, dissolved tungsten ions, which caused an increase of the specific methane production, up to 63% under mesophilic and 31% under thermophilic conditions. That is the same optimal range for pure cultures of methanogens or bacteria found in literature. A simultaneous decrease of volatile fatty acids occurred. The Ni/W effect occurred with all three organic loading rates, being 4.5, 7.5, and 9.0 g volatile solids per litre and day, with a concomitant hydraulic retention time of 18, 10, or 8 days, respectively. A maximum specific methane production of 0.254 m3 CH4, under standard temperature and pressure per kg volatile solids (almost 90% degradation), was obtained. After the final supplementation of tungsten, the cell counts of methanogens increased by 300%, while the total microbial cell counts increased by only 3–62%. The mesophilic methanogenic microflora was shifted from the acetotrophic Methanosaeta to the hydrogenotrophic Methanoculleus (85%) by tungsten, whereas the H2-CO2-converter, Methanothermobacter, always dominated in the thermophilic fermenters.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Mesophilic and Thermophilic Anaerobic Digestion of Wheat Straw in a CSTR System with ‘Synthetic Manure’: Impact of Nickel and Tungsten on Methane Yields, Cell Count, and Microbiome

et al. 2022

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“…In addition, fermentative microorganisms may also have the capability to produce hydrogen. In such energy-poor environments, hydrogen is assumed to be rapidly consumed by other community members, such as hydrogenotrophic methanogens (with less than 0.55% ASVs), syntrophic acetate-oxidizing bacteria (Firmicutes DTU014; Dyksma et al, 2020 ), homoacetogens (“Ca. Acetothermia”; Youssef et al, 2019 ), or hydrogenotrophic sulfate-reducing bacteria ( Desulfomonile , Desulfovibrio , and Desulfotomaculum ; DeWeerd et al, 1991 ; Voordouw, 1995 ; Aüllo et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Microbial Diversity Under the Influence of Natural Gas Storage in a Deep Aquifer

et al. 2021

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Deep aquifers (up to 2km deep) contain massive volumes of water harboring large and diverse microbial communities at high pressure. Aquifers are home to microbial ecosystems that participate in physicochemical balances. These microorganisms can positively or negatively interfere with subsurface (i) energy storage (CH4 and H2), (ii) CO2 sequestration; and (iii) resource (water, rare metals) exploitation. The aquifer studied here (720m deep, 37°C, 88bar) is naturally oligotrophic, with a total organic carbon content of <1mg.L−1 and a phosphate content of 0.02mg.L−1. The influence of natural gas storage locally generates different pressures and formation water displacements, but it also releases organic molecules such as monoaromatic hydrocarbons at the gas/water interface. The hydrocarbon biodegradation ability of the indigenous microbial community was evaluated in this work. The in situ microbial community was dominated by sulfate-reducing (e.g., Sva0485 lineage, Thermodesulfovibriona, Desulfotomaculum, Desulfomonile, and Desulfovibrio), fermentative (e.g., Peptococcaceae SCADC1_2_3, Anaerolineae lineage and Pelotomaculum), and homoacetogenic bacteria (“Candidatus Acetothermia”) with a few archaeal representatives (e.g., Methanomassiliicoccaceae, Methanobacteriaceae, and members of the Bathyarcheia class), suggesting a role of H2 in microenvironment functioning. Monoaromatic hydrocarbon biodegradation is carried out by sulfate reducers and favored by concentrated biomass and slightly acidic conditions, which suggests that biodegradation should preferably occur in biofilms present on the surfaces of aquifer rock, rather than by planktonic bacteria. A simplified bacterial community, which was able to degrade monoaromatic hydrocarbons at atmospheric pressure over several months, was selected for incubation experiments at in situ pressure (i.e., 90bar). These showed that the abundance of various bacterial genera was altered, while taxonomic diversity was mostly unchanged. The candidate phylum Acetothermia was characteristic of the community incubated at 90bar. This work suggests that even if pressures on the order of 90bar do not seem to select for obligate piezophilic organisms, modifications of the thermodynamic equilibria could favor different microbial assemblages from those observed at atmospheric pressure.

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“…It is noteworthy that some genera present in low abundance (≤0.5%) in the inoculum, e.g., Syntrophaceticus, Sphaerobacter, Thermacetogenium, and Mycobacterium also managed to avoid extinction under the starvation conditions [67][68][69][70]. Some of these and the genus Acetomicrobium exhibited similar behavior and are suspected or verified SAOBs [71].…”

Section: Discussionmentioning

confidence: 99%

Development of Stable Mixed Microbiota for High Yield Power to Methane Conversion

et al. 2021

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The performance of a mixed microbial community was tested in lab-scale power-to-methane reactors at 55 °C. The main aim was to uncover the responses of the community to starvation and stoichiometric H2/CO2 supply as the sole substrate. Fed-batch reactors were inoculated with the fermentation effluent of a thermophilic biogas plant. Various volumes of pure H2/CO2 gas mixtures were injected into the headspace daily and the process parameters were followed. Gas volumes and composition were measured by gas-chromatography, the headspace was replaced with N2 prior to the daily H2/CO2 injection. Total DNA samples, collected at the beginning and end (day 71), were analyzed by metagenome sequencing. Low levels of H2 triggered immediate CH4 evolution utilizing CO2/HCO3− dissolved in the fermentation effluent. Biomethanation continued when H2/CO2 was supplied. On the contrary, biomethane formation was inhibited at higher initial H2 doses and concomitant acetate formation indicated homoacetogenesis. Biomethane production started upon daily delivery of stoichiometric H2/CO2. The fed-batch operational mode allowed high H2 injection and consumption rates albeit intermittent operation conditions. Methane was enriched up to 95% CH4 content and the H2 consumption rate attained a remarkable 1000 mL·L−1·d−1. The microbial community spontaneously selected the genus Methanothermobacter in the enriched cultures.

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Syntrophic acetate oxidation replaces acetoclastic methanogenesis during thermophilic digestion of biowaste

Cited by 156 publications

References 86 publications

Mesophilic and Thermophilic Anaerobic Digestion of Wheat Straw in a CSTR System with ‘Synthetic Manure’: Impact of Nickel and Tungsten on Methane Yields, Cell Count, and Microbiome

Mesophilic and Thermophilic Anaerobic Digestion of Wheat Straw in a CSTR System with ‘Synthetic Manure’: Impact of Nickel and Tungsten on Methane Yields, Cell Count, and Microbiome

Microbial Diversity Under the Influence of Natural Gas Storage in a Deep Aquifer

Development of Stable Mixed Microbiota for High Yield Power to Methane Conversion

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