Synthetic sludge: A physical/chemical model in understanding bioflocculation

Sanin, F. Dilek; Vesilind, P. Aarne

doi:10.2175/106143096x127938

Cited by 59 publications

(42 citation statements)

References 13 publications

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“…Bridging causes an increase in particle aggregation rate due to more effective particle collisions and a decrease in aggregate break-up rate. Examples of chemicals that can be used for particle size manipulation are borax, which is an effective flocculation agent for cell debris, as reported by Tsoka et al (2000) and calcium ions that can be used to flocculate microorganisms by forming metal-polymer complexes with the polymers on the surface of the microorganism, as reported by Sanin and Vesilind (1996).…”

Section: Optimization and Control Of Driving Forcesmentioning

confidence: 99%

Strategy for selection of methods for separation of bioparticles from particle mixtures

Hee

Hoeben

Lans

et al. 2006

Biotech & Bioengineering

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The desired product of bioprocesses is often produced in particulate form, either as an inclusion body (IB) or as a crystal. Particle harvesting is then a crucial and attractive form of product recovery. Because the liquid phase often contains other bioparticles, such as cell debris, whole cells, particulate biocatalysts or particulate by-products, the recovery of product particles is a complex process. In most cases, the particulate product is purified using selective solubilization or extraction. However, if selective particle recovery is possible, the already high purity of the particles makes this downstream process more favorable. This work gives an overview of typical bioparticle mixtures that are encountered in industrial biotechnology and the various driving forces that may be used for particle-particle separation, such as the centrifugal force, the magnetic force, the electric force, and forces related to interfaces. By coupling these driving forces to the resisting forces, the limitations of using these driving forces with respect to particle size are calculated. It shows that centrifugation is not a general solution for particle-particle separation in biotechnology because the particle sizes of product and contaminating particles are often very small, thus, causing their settling velocities to be too low for efficient separation by centrifugation. Examples of such separation problems are the recovery of IBs or virus-like particles (VLPs) from (microbial) cell debris. In these cases, separation processes that use electrical forces or fluid-fluid interfaces show to have a large potential for particle-particle separation. These methods are not yet commonly applied for large-scale particle-particle separation in biotechnology and more research is required on the separation techniques and on particle characterization to facilitate successful application of these methods in industry.

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Section: Optimization and Control Of Driving Forcesmentioning

confidence: 99%

Strategy for selection of methods for separation of bioparticles from particle mixtures

Hee

Hoeben

Lans

et al. 2006

Biotech & Bioengineering

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show abstract

“…However, aggregates occurring naturally may have multilevel structure (19)(20)(21). Gorczyca and Ganczarczyk (22) proposed an activated sludge model that includes the following stages: (1) primary particles form flocculi, (2) flocculi group together to form microflocs, and (3) microflocs form the flocs.…”

Section: Introductionmentioning

confidence: 99%

Multilevel Structure of Sludge Flocs

Lee

Waite

et al. 2002

Journal of Colloid and Interface Science

142

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“…11 Ca 2+ and Mg 2+ seem to be the most important cations for bridging organic substances and microorganisms in waste sludge 12 and also for fl oc formation and fl occulation of bacteria. [13][14][15][16] These results indicate that supernatant water from solubilized sludge can act as a biofl occulant. Methane production from the supernatant water with incubation time (solubilizing conditions: reagent, acetic acid; time, 1 h; temperature, 80°C; reagent concentration, 0.5 kmol/m 3 ; sludge volume, 20 ml; reagent volume, 5.0 ml).…”

Section: Resultsmentioning

confidence: 80%

Resource recovery treatment of waste sludge using a solubilizing reagent

Nomura

Araki

Nagao

et al. 2007

J Mater Cycles Waste Manag

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The dewatering of waste sludge continues to be a major problem in wastewater treatment. In this study, the solubilization treatment of waste sludge and extracellular polymeric substances using a solubilizing reagent was examined experimentally. For this purpose, a compression test of thickened waste sludge obtained after solubilization treatment was carried out. The total solid content of the dewatered cake was over 30% when using hydrochloric acid or acetic acid as the solubilizing reagent; however, it was about 20% when using sodium hydroxide. The thickened waste sludge was effectively solubilized when the concentration of acetic acid in the sludge, assuming that it was diluted by free water and not bound water in the sludge, was greater than 0.3 kmol/m 3 . A fl occulated sedimentation test using the supernatant water after solubilization treatment was also carried out, revealing that it functions in a similar manner to commercial fl occulant in aggregating solid particles under gravity. This result indicates that the supernatant water can act as a biofl occulant. Methane fermentation of the supernatant water was subsequently carried out. The fi ndings showed that by using acetic acid as a solubilizing reagent, solubilized organic substances in the supernatant water could be recovered as a bioenergy resource.

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Synthetic sludge: A physical/chemical model in understanding bioflocculation

Cited by 59 publications

References 13 publications

Strategy for selection of methods for separation of bioparticles from particle mixtures

Strategy for selection of methods for separation of bioparticles from particle mixtures

Multilevel Structure of Sludge Flocs

Resource recovery treatment of waste sludge using a solubilizing reagent

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