Synthetic Salicylic acid inducible recombinant promoter for translational research

Deb, Debasish; Dey, Nrisingha

doi:10.1016/j.jbiotec.2019.03.004

Cited by 11 publications

(11 citation statements)

References 61 publications

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“…Similarly, the synthetic promoter FUASCsV8C was designed by placing the upstream activation sequence (UAS) of the figwort mosaic virus (FMV; −249 to −54) at the 5 ′ -end of the cassava vein mosaic virus (CsVMV) promoter fragment 8 (CsVMV8; −215 to +166). This hybridized, synthetic promoter exhibited approximately 2.1-times higher GUS activities in tobacco protoplasts and 2.0-times higher activity in agroinfiltration assays, compared to the CaMV35S promoter [222].…”

Section: Cis-element Juggling and Domain Swappingmentioning

confidence: 89%

“…The latter approach is often practiced as ligating the upstream activation sequence (UAS) from one promoter to the TATA box-containing domain of another promoter. It is expected that the transfer of the cis-element or upstream DNA sequence from one promoter into another containing the TATA sequence could provide a novel transcription regulatory mechanism [128,181,222].…”

Section: Cis-element Juggling and Domain Swappingmentioning

confidence: 99%

“…These relatively weaker synthetic promoters could be important for complex metabolic engineering approaches, where the decreased or precisely tuned load of heterologously expressed genes could be salutary to host primary metabolic routes [195,[223][224][225][226][227]. Further applications of domain hybridization generated many superior plant promoters [128,181,209,222,[228][229][230].…”

Section: Cis-element Juggling and Domain Swappingmentioning

confidence: 99%

“…Viral and plant pathogen genomes are relatively simple and easily available sources of naturally occurring cis-active elements that can be used to prepare novel synthetic promoters through domain hybridization [181,183,222,230]. These synthetic promoters could indicate the higher activity of CAMV35S.…”

Section: Cis-element Juggling and Domain Swappingmentioning

confidence: 99%

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Plant Synthetic Promoters

Szymczyk,

Majewska

2024

Applied Sciences

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This article examines the structure and functions of the plant synthetic promoters frequently used to precisely regulate complex regulatory routes. It details the composition of native promoters and their interacting proteins to provide a better understanding of the tasks associated with synthetic promoter development. The production of synthetic promoters is performed by relatively small libraries produced generally by basic molecular or genetic engineering methods such as cis-element shuffling or domain swapping. The article also describes the preparation of large-scale libraries supported by synthetic DNA fragments, directed evolution, and machine or deep-learning methodologies. The broader application of novel, synthetic promoters reduces the prevalence of homology-based gene silencing or improves the stability of transgenes. A particularly interesting group of synthetic promoters are bidirectional forms, which can enable the expression of up to eight genes by one regulatory element. The introduction and controlled expression of several genes after one transgenic event strongly decreases the frequency of such problems as complex segregation patterns and the random integration of multiple transgenes. These complications are commonly observed during the transgenic crop development enabled by traditional, multistep transformation using genetic constructs containing a single gene. As previously tested DNA promoter fragments demonstrate low complexity and homology, their abundance can be increased by using orthogonal expression systems composed of synthetic promoters and trans-factors that do not occur in nature or arise from different species. Their structure, functions, and applications are rendered in the article. Among them are presented orthogonal systems based on transcription activator-like effectors (dTALEs), synthetic dTALE activated promoters (STAPs) and dCas9-dependent artificial trans-factors (ATFs). Synthetic plant promoters are valuable tools for providing precise spatiotemporal regulation and introducing logic gates into the complex genetic traits that are important for basic research studies and their application in crop plant development. Precisely regulated metabolic routes are less prone to undesirable feedback regulation and energy waste, thus improving the efficiency of transgenic crops.

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Section: Cis-element Juggling and Domain Swappingmentioning

confidence: 89%

Section: Cis-element Juggling and Domain Swappingmentioning

confidence: 99%

Section: Cis-element Juggling and Domain Swappingmentioning

confidence: 99%

Section: Cis-element Juggling and Domain Swappingmentioning

confidence: 99%

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Plant Synthetic Promoters

Szymczyk,

Majewska

2024

Applied Sciences

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“…In the last two decades, redesigning promoter structure by "Cis-rearrangement" and "shuffling of sub-domains" has acquired importance in maintaining robust gene expression in plants against abiotic and biotic stresses. [10,[28][29][30][31][32][33][34][35][36][37]. Such a genetically manipulated transcriptional unit is constructed by employing several state-of-the-art molecular strategies, including promoter DNA shuffling, hybridization, domain exchange, domain duplication, site-directed mutagenesis, etc.…”

Section: Introductionmentioning

confidence: 99%

Plant Synthetic Promoters: Advancement and Prospective

et al. 2023

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Native/endogenous promoters have several fundamental limitations in terms of their size, Cis-elements distribution/patterning, and mode of induction, which is ultimately reflected in their insufficient transcriptional activity. Several customized synthetic promoters were designed and tested in plants during the past decade to circumvent such constraints. Such synthetic promoters have a built-in capacity to drive the expression of the foreign genes at their maximum amplitude in plant orthologous systems. The basic structure and function of the promoter has been discussed in this review, with emphasis on the role of the Cis-element in regulating gene expression. In addition to this, the necessity of synthetic promoters in the arena of plant biology has been highlighted. This review also provides explicit information on the two major approaches for developing plant-based synthetic promoters: the conventional approach (by utilizing the basic knowledge of promoter structure and Cis-trans interaction) and the advancement in gene editing technology. The success of plant genetic manipulation relies on the promoter efficiency and the expression level of the transgene. Therefore, advancements in the field of synthetic promoters has enormous potential in genetic engineering-mediated crop improvement.

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