Synthetic peptides with selective affinity for apoptotic cells

DiVittorio, Kristy M.; Johnson, James R.; Johansson, Emma; Reynolds, Aaron; Jolliffe, Katrina A.; Smith, Bradley D.

doi:10.1039/b514748d

Cited by 33 publications

(34 citation statements)

References 28 publications

(30 reference statements)

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“…Two of Zn 2? -DPA molecules can be also attached to side groups of fluorescein-labeled short peptides for increasing the strength of membrane binding (DiVittorio et al 2006). Substitution of fluorescein by a near-IR fluorescence reporter allowed extending the imaging possibilities (Smith et al 2011a).…”

Section: The Unlimited Choice Of Dyes Exists For Its Labelingmentioning

confidence: 99%

Beyond annexin V: fluorescence response of cellular membranes to apoptosis

2012

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Dramatic changes in the structure of cell membranes on apoptosis allow easy, sensitive and non-destructive analysis of this process with the application of fluorescence methods. The strong plasma membrane asymmetry is present in living cells, and its loss on apoptosis is commonly detected with the probes interacting strongly and specifically with phosphatidylserine (PS). This phospholipid becomes exposed to the cell surface, and the application of annexin V labeled with fluorescent dye is presently the most popular tool for its detection. Several methods have been suggested recently that offer important advantages over annexin V assay with the ability to study apoptosis by spectroscopy of cell suspensions, flow cytometry and confocal or twophoton microscopy. The PS exposure marks the integrated changes in the outer leaflet of cell membrane that involve electrostatic potential and hydration, and the attempts are being made to provide direct probing of these changes. This review describes the basic mechanisms underlying the loss of membrane asymmetry during apoptosis and discusses, in comparison with the annexin V-binding assay, the novel fluorescence techniques of detecting apoptosis on cellular membrane level. In more detail we describe the detection method based on smart fluorescent dye F2N12S incorporated into outer leaflet of cell membrane and reporting on apoptotic cell transformation by easily detectable change of the spectral distribution of fluorescent emission. It can be adapted to any assay format.

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Section: The Unlimited Choice Of Dyes Exists For Its Labelingmentioning

confidence: 99%

Beyond annexin V: fluorescence response of cellular membranes to apoptosis

2012

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“…Zn-Dpa complex 1 was prepared as reported previously (Scheme 1) 14 and characterized, among others, by the downfield shifts of the pyridine protons of 1 from those of 2 in 1 H NMR (Fig. S1).…”

Section: Complex Formation Of 2 With Zn 2+mentioning

confidence: 99%

Discrimination of phosphorylated double stranded DNA by naphthalene diimide having zinc(II) dipicolylamine complexes

Watanabe

Ohtsuka

Sato

et al. 2011

Bioorganic & Medicinal Chemistry

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“…133 In addition, peptides specific to molecules do have advantages over protein probes for a number of reasons, including superior stability, non-immunogenicity, low cost, easy labeling and rapid clearance and tissue penetration. 134,135 The bacteriophage (phage) display technology was used for identification of peptide-based targeting agents. A phage display library is selected against a desired target (purified antigen, cell line, live organism) to find the fittest subpopulation, which is then amplified and reselected again.…”

Section: Imaging Apoptosis With Other Ps Binding Proteinsmentioning

confidence: 99%