Synthetic Peptides as Diagnostic Tools in Virology

Leinikki, Pauli; Lehtinen, Matti; Hyöty, Heikki; Parkkonen, Päivi; Kantanen, Marja-Leena; Hakulinen, Juha

doi:10.1016/s0065-3527(08)60085-8

Cited by 51 publications

(21 citation statements)

References 158 publications

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“…Peptides identified in this manner have many applications, for instance as diagnostic reagents to replace infectious agents in immunoassays, 14 as immunogens to obtain antibody reagents useful for isolating gene products 15 and as potential synthetic vaccines. 16,17 This explains why most attempts at predicting protein epitopes have concentrated on continuous epitopes.…”

Section: The Nature Of B Cell Epitopesmentioning

confidence: 99%

Immunoinformatics may lead to a reappraisal of the nature of B cell epitopes and of the feasibility of synthetic peptide vaccines

Regenmortel

2006

J of Molecular Recognition

102

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Section: The Nature Of B Cell Epitopesmentioning

confidence: 99%

Immunoinformatics may lead to a reappraisal of the nature of B cell epitopes and of the feasibility of synthetic peptide vaccines

Regenmortel

2006

J of Molecular Recognition

102

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“…The classification of antigenic determinants into epitopes and nonepitopes ignoring the antigen reconfiguration in Ag-Ab complex may not accurately reflect biological reality [3]. The accurate identification of B-cell epitopes constitutes a basis for development of antibody therapeutics [4], peptide-based vaccines [4, 5], and immunodiagnostic tool [6]. …”

Section: Introductionmentioning

confidence: 99%

An Introduction to B-Cell Epitope Mapping and In Silico Epitope Prediction

Potocnakova

Bhide

Pulzová

2016

Journal of Immunology Research

261

193

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Identification of B-cell epitopes is a fundamental step for development of epitope-based vaccines, therapeutic antibodies, and diagnostic tools. Epitope-based antibodies are currently the most promising class of biopharmaceuticals. In the last decade, in-depth in silico analysis and categorization of the experimentally identified epitopes stimulated development of algorithms for epitope prediction. Recently, various in silico tools are employed in attempts to predict B-cell epitopes based on sequence and/or structural data. The main objective of epitope identification is to replace an antigen in the immunization, antibody production, and serodiagnosis. The accurate identification of B-cell epitopes still presents major challenges for immunologists. Advances in B-cell epitope mapping and computational prediction have yielded molecular insights into the process of biorecognition and formation of antigen-antibody complex, which may help to localize B-cell epitopes more precisely. In this paper, we have comprehensively reviewed state-of-the-art experimental methods for B-cell epitope identification, existing databases for epitopes, and novel in silico resources and prediction tools available online. We have also elaborated new trends in the antibody-based epitope prediction. The aim of this review is to assist researchers in identification of B-cell epitopes.

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“…Epitopes are typically identified and characterized to apply this knowledge for detailed functional characterization of an antibody (Al‐Majdoub et al, ) use in disease diagnostics (Leinikki et al, ; Andresen & Grotzinger, ), and medical treatments in which epitope peptides are intended to be used as therapeutics, for example, as peptide‐based vaccines (Muller et al, ). …”

Section: Discussionmentioning

confidence: 99%

Mass spectrometric epitope mapping

Opuni

Al-Majdoub

Yefremova

et al. 2016

Mass Spectrometry Reviews

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Mass spectrometric epitope mapping has become a versatile method to precisely determine a soluble antigen's partial structure that directly interacts with an antibody in solution. Typical lengths of investigated antigens have increased up to several 100 amino acids while experimentally determined epitope peptides have decreased in length to on average 10-15 amino acids. Since the early 1990s more and more sophisticated methods have been developed and have forwarded a bouquet of suitable approaches for epitope mapping with immobilized, temporarily immobilized, and free-floating antibodies. While up to now monoclonal antibodies have been mostly used in epitope mapping experiments, the applicability of polyclonal antibodies has been proven. The antibody's resistance towards enzymatic proteolysis has been of key importance for the two mostly applied methods: epitope excision and epitope extraction. Sample consumption has dropped to low pmol amounts on both, the antigen and the antibody. While adequate in-solution sample handling has been most important for successful epitope mapping, mass spectrometric analysis has been found the most suitable read-out method from early on. The rapidity by which mass spectrometric epitope mapping nowadays is executed outperforms all alternative methods. Thus, it can be asserted that mass spectrometric epitope mapping has reached a state of maturity, which allows it to be used in any mass spectrometry laboratory. After 25 years of constant and steady improvements, its application to clinical samples, for example, for patient characterization and stratification, is anticipated in the near future. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 37:229-241, 2018.

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Synthetic Peptides as Diagnostic Tools in Virology

Cited by 51 publications

References 158 publications

Immunoinformatics may lead to a reappraisal of the nature of B cell epitopes and of the feasibility of synthetic peptide vaccines

Immunoinformatics may lead to a reappraisal of the nature of B cell epitopes and of the feasibility of synthetic peptide vaccines

An Introduction to B-Cell Epitope Mapping and In Silico Epitope Prediction

Mass spectrometric epitope mapping

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