Synthetic peptide vaccines

Moisa, A. A.; Колесанова, Е. Ф.

doi:10.18097/pbmc20115701104

Cited by 10 publications

(2 citation statements)

References 91 publications

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“…Amino acids in boldface type are those in rat HER2/neu, which are different from those in HER2/neu murine sequence . The goal of the current study was to overcome the peptide vaccine limitations, such as its weak immunogenicity , binding to non‐professional APCs, and rapid degradation by tissue and serum peptidases . Here, DCs were prepared in vitro from mouse bone marrow stem cells.…”

Section: Introductionmentioning

confidence: 99%

Enhanced immune response induced by P5 HER2/neu‐derived peptide‐pulsed dendritic cells as a preventive cancer vaccine

Gholizadeh

Tavakkol‐Afshari

Nikpoor

et al. 2017

J Cellular Molecular Medi

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Dendritic cells are special and powerful antigen‐presenting cells that can induce primary immune responses against tumour‐associated antigens. They can present antigens via both MHC‐I and MHC‐II, so they have the ability to stimulate both cytotoxic T lymphocytes and T helper cells. Furthermore, CD8+ cytotoxic T lymphocytes require activation by CD4+ T cells. This requires a CD4+T cell activator molecule, of which PADRE is one of the best. We chose an approach to use both of these important arms of the immune system. We prepared dendritic cells from mouse bone marrow, loaded them with our target peptides (P5 peptide alone or P5 + PADRE), and then injected these pulsed dendritic cells alone or in combination with CpG‐ODN (as adjuvant) into BALB/C mice. After the last boosting dose, mice were inoculated with TUBO cells, which overexpress HER2/neu. Two weeks after the tumour cell injection, immunological tests were performed on splenocyte suspensions, and the remaining mice were evaluated for tumour growth and survival. Our data indicate the formulation that contains PADRE plus P5 loaded onto DC in combination with CpG‐ODN was the most effective formulation at inducing immune responses. Interferon production in CD4+ and CD8+ gated cells, cytotoxicity rates of target cells and mice survival were all significantly greater in this group than in controls, and all the mice in this group were tumour‐free throughout the experiment. Based on our results and the role of HER2/neu as a candidate in human immunotherapy, this approach may be an effective cancer treatment.

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Section: Introductionmentioning

confidence: 99%

Enhanced immune response induced by P5 HER2/neu‐derived peptide‐pulsed dendritic cells as a preventive cancer vaccine

Gholizadeh

Tavakkol‐Afshari

Nikpoor

et al. 2017

J Cellular Molecular Medi

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“…Aside from introducing excessive non immune-stimulating pathogenic material into the patient, drawbacks of all clinically applied vaccines include poor or incomplete inactivation, reversion of virulence and limitation to viral pathogens [4]. Utilizing synthetic peptides as antigen mimetics bear the promise to avert some of these effects [5]–[7]. The importance of prediction and design of major histocompatibility protein I (MHC-I) bound peptides that are recognized as a complex by receptors on cytotoxic T cells (cell-mediated immunity) was outlined by Rammensee and co-workers in 1993, who defined canonical sequence motifs for a set of MHC-I alleles (Fig.…”

Section: Introductionmentioning

confidence: 99%

Scrutinizing MHC-I Binding Peptides and Their Limits of Variation

et al. 2013

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Designed peptides that bind to major histocompatibility protein I (MHC-I) allomorphs bear the promise of representing epitopes that stimulate a desired immune response. A rigorous bioinformatical exploration of sequence patterns hidden in peptides that bind to the mouse MHC-I allomorph H-2Kb is presented. We exemplify and validate these motif findings by systematically dissecting the epitope SIINFEKL and analyzing the resulting fragments for their binding potential to H-2Kb in a thermal denaturation assay. The results demonstrate that only fragments exclusively retaining the carboxy- or amino-terminus of the reference peptide exhibit significant binding potential, with the N-terminal pentapeptide SIINF as shortest ligand. This study demonstrates that sophisticated machine-learning algorithms excel at extracting fine-grained patterns from peptide sequence data and predicting MHC-I binding peptides, thereby considerably extending existing linear prediction models and providing a fresh view on the computer-based molecular design of future synthetic vaccines. The server for prediction is available at http://modlab-cadd.ethz.ch (SLiDER tool, MHC-I version 2012).

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Production of Antibodies to Peptide Targets Using Hybridoma Technology

Trier,

Friis

2024

Methods in Molecular Biology

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Synthetic peptide vaccines

Cited by 10 publications

References 91 publications

Enhanced immune response induced by P5 HER2/neu‐derived peptide‐pulsed dendritic cells as a preventive cancer vaccine

Enhanced immune response induced by P5 HER2/neu‐derived peptide‐pulsed dendritic cells as a preventive cancer vaccine

Scrutinizing MHC-I Binding Peptides and Their Limits of Variation

Production of Antibodies to Peptide Targets Using Hybridoma Technology

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