Synthetic MicroProteins: Versatile Tools for Posttranslational Regulation of Target Proteins

Dolde, Ulla; Rodrigues, Vandasue; Straub, Daniel; Bhati, Kaushal Kumar; Choi, Sukwon; Yang, Seong Wook; Wenkel, Stephan

doi:10.1104/pp.17.01743

Cited by 22 publications

(32 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another role for neofunctionalized truncated genes may be regulation of full‐length variants via competitive inhibition at the protein level (Seo et al , ; Graeff et al , ). Truncated proteins called microproteins can heterodimerize with paralogous full‐length proteins to act as interfering regulators (Straub & Wenkel, ; Dolde et al , ). Some of the truncated and small fragment genes we found resemble microproteins.…”

Section: Discussionmentioning

confidence: 99%

Structural evolution drives diversification of the large LRR‐RLK gene family

2020

View full text Add to dashboard Cite

Cells are continuously exposed to chemical signals that they must discriminate between and respond to appropriately. In embryophytes, the leucine-rich repeat receptor-like kinases (LRR-RLKs) are signal receptors critical in development and defense. LRR-RLKs have diversified to hundreds of genes in many plant genomes. Although intensively studied, a well-resolved LRR-RLK gene tree has remained elusive.To resolve the LRR-RLK gene tree, we developed an improved gene discovery method based on iterative hidden Markov model searching and phylogenetic inference. We used this method to infer complete gene trees for each of the LRR-RLK subclades and reconstructed the deepest nodes of the full gene family.We discovered that the LRR-RLK gene family is even larger than previously thought, and that protein domain gains and losses are prevalent. These structural modifications, some of which likely predate embryophyte diversification, led to misclassification of some LRR-RLK variants as members of other gene families. Our work corrects this misclassification.Our results reveal ongoing structural evolution generating novel LRR-RLK genes. These new genes are raw material for the diversification of signaling in development and defense. Our methods also enable phylogenetic reconstruction in any large gene family.

show abstract

Section: Discussionmentioning

confidence: 99%

Structural evolution drives diversification of the large LRR‐RLK gene family

2020

View full text Add to dashboard Cite

show abstract

“…The inhibition of the long day repression mechanism of rice might have beneficial implications in the expansion of rice cultivation in equatorial regions and areas of northern latitudes. Synthetic microProteins are proving to be a useful tool in targeting and inhibiting proteins in a more specific manner with the reduced likelihood of an off‐target effect (Bhati et al 2018; Dolde et al 2018). Furthermore, synthetic microProteins can also aid in deciphering what processes are dependent on the activity of the inhibited target protein.…”

Section: Resultsmentioning

confidence: 99%

Control of flowering in rice through synthetic microProteins

Eguen

Ariza

Brambilla

et al. 2019

JIPB

Self Cite

View full text Add to dashboard Cite

Photoperiod-dependent flowering in rice is regulated by HEADING DATE 1 (Hd1), which acts as both an activator and repressor of flowering in a daylengthdependent manner. To investigate the use of micro-Proteins as a tool to modify rice sensitivity to the photoperiod, we designed a synthetic Hd1 microProtein (Hd1miP) capable of interacting with Hd1 protein, and overexpressed it in rice. Transgenic OX-Hd1miP plants flowered significantly earlier than wild type plants when grown in non-inductive long day conditions. Our results show the potential of microProteins to serve as powerful tools for modulating crop traits and unraveling protein function.

show abstract

“…The ability of proteins that lack particular domains to act as dominant negative regulators of their interaction partners is being exploited to modulate plant development (Seo et al, 2012;Dolde et al, 2018). For example, overexpression of engineered microProteins that interact with the MADS-box transcription factor AGAMOUS act in a dominant negative fashion, producing phenotypes similar to agamous null mutants (Seo et al, 2012).…”

Section: Domain Loss Creates Negative Regulators Of Transcriptionmentioning

confidence: 99%

“…Tansley insight New Phytologist protein BRI1 to the membrane, rendering plants insensitive to brassinosteroids (Dolde et al, 2018). Thus, overexpression of particular microProteins in specific developmental contexts can be used for precisely regulating developmental processes.…”

Section: Reviewmentioning

confidence: 99%

Looking back to look forward: protein–protein interactions and the evolution of development

Bartlett

2019

New Phytologist

View full text Add to dashboard Cite

The evolutionary modification of development was fundamental in generating extant plant diversity. Similarly, the modification of development is a path forward to engineering the plants of the future, provided we know enough about what to modify. Understanding how extant diversity was generated will reveal productive pathways forward for modifying development.Here, I discuss four examples of developmental pathways that have been remodeled by changes to protein-protein interactions. These are cases where changes to developmental pathways have been paralleled by recent changes, selected for or engineered by humans. Extant plant diversity represents a vast treasure trove of molecular solutions to ecological problems. Mining this treasure trove will allow for the intentional modification of plant development for solving future problems.

show abstract

Synthetic MicroProteins: Versatile Tools for Posttranslational Regulation of Target Proteins

Cited by 22 publications

References 48 publications

Structural evolution drives diversification of the large LRR‐RLK gene family

Structural evolution drives diversification of the large LRR‐RLK gene family

Control of flowering in rice through synthetic microProteins

Looking back to look forward: protein–protein interactions and the evolution of development

Contact Info

Product

Resources

About