Synthetic Melanin Suppresses Production of Proinflammatory Cytokines

Mohagheghpour, Nahid; Waleh, Nahid; Garger, Stephen J.; Dousman, Linda; Grill, Laurence K.; Tusé, Daniel

doi:10.1006/cimm.1999.1599

Cited by 89 publications

(72 citation statements)

References 37 publications

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“…Mohagheghpour et al 46) showed that synthetic melanin, produced by tyrosinase oxidation of tyrosine, diminished the production of some cytokines, including IL-1, IL-6, and TNF-α, and showed anti-inflammatory properties. Wang and Mazza 47) showed that various polyphenolics, compounds structurally similar to melanin, diminished the production of NO in macrophages stimulated with IFN-γ and LPS.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Nitric Oxide and Tumour Necrosis Factor-α Production in Peritoneal Macrophages by <i>Aspergillus nidulans</i> Melanin

Gonçalves

Kitagawa

Raddi

et al. 2013

Biological & Pharmaceutical Bulletin

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The naturally occurring pigment, melanin is found in organisms of all phylogenetic kingdoms, including fungi, and exhibits a wide range of biological activities. Our objective was to investigate the effects of melanin extracted from the fungus Aspergillus nidulans on the production of the pro-inflammatory mediators nitric oxide (NO) and tumour necrosis factor-α (TNF-α) in peritoneal macrophages and on the viability of McCoy mouse fibroblasts. The results showed that A. nidulans melanin did not stimulate NO production in macrophages, but it inhibited the NO production in lipopolysaccharide (LPS)-stimulated macrophages by approximately 82%. Similarly, A. nidulans melanin inhibited LPS-stimulated TNF-α production by 52% and showed a slight stimulatory effect on TNF-α production in macrophages. In addition, the toxicity of A. nidulans melanin to McCoy cells was much lesser (IC 50 373.5 2.4 µg/mL) than that of known agents such as cisplatin (IC 50 41.2 µg/mL). The viability of peritoneal macrophages was greater than 90% at the highest melanin concentration tested (100 µg/mL). Thus, the combination of low cytotoxicity and marked inhibition of TNF-α and NO production suggests that A. nidulans melanin has potential as an anti-inflammatory agent and may be used in the future for development of new drugs with therapeutic utility.

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Section: Resultsmentioning

confidence: 99%

Inhibition of Nitric Oxide and Tumour Necrosis Factor-α Production in Peritoneal Macrophages by <i>Aspergillus nidulans</i> Melanin

Gonçalves

Kitagawa

Raddi

et al. 2013

Biological & Pharmaceutical Bulletin

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“…After 30 min the medium was changed, and cells were incubated in methionine-and cysteine-free medium in the presence of 10% dialyzed FCS and 5 g/ml BFA for 10 min. Then M-CSF was added to the cultures together with 100 Ci/ml 35 (28,29), after a 10-min incubation with BFA, 50 ng/ml lipopolysaccharide was added to the cultures together with 100 Ci/ml 35 S-labeled methionine and cysteine mixture. After 2 h cells were washed twice with ice-cold PBS.…”

Section: Methodsmentioning

confidence: 99%

Toll-like Receptor 9 Regulates Tumor Necrosis Factor-α Expression by Different Mechanisms

Amcheslavsky

Zou

Bar‐Shavit

2004

Journal of Biological Chemistry

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“…In vivo, Phe is a precursor of melanin [15]. Mohagheghpour et al [16] found that melanin could inhibit the production of cytokines such as interleukin-1b (IL-1b), interleukin-6 (IL-6) and TNF-a in human peripheral blood monocytes. These data indicated a possible correlation between Phe and cytokine production in fish, which warrants further study.…”

Section: Introductionmentioning

confidence: 99%

Dietary phenylalanine-improved intestinal barrier health in young grass carp (Ctenopharyngodon idella) is associated with increased immune status and regulated gene expression of cytokines, tight junction proteins, antioxidant enzymes and related signalling molecules

Feng

Wen

Liu

et al. 2015

Fish & Shellfish Immunology

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Synthetic Melanin Suppresses Production of Proinflammatory Cytokines

Cited by 89 publications

References 37 publications

Inhibition of Nitric Oxide and Tumour Necrosis Factor-α Production in Peritoneal Macrophages by <i>Aspergillus nidulans</i> Melanin

Inhibition of Nitric Oxide and Tumour Necrosis Factor-α Production in Peritoneal Macrophages by <i>Aspergillus nidulans</i> Melanin

Toll-like Receptor 9 Regulates Tumor Necrosis Factor-α Expression by Different Mechanisms

Dietary phenylalanine-improved intestinal barrier health in young grass carp (Ctenopharyngodon idella) is associated with increased immune status and regulated gene expression of cytokines, tight junction proteins, antioxidant enzymes and related signalling molecules

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