Synthetic lipid A with endotoxic and related biological activities, comparable to a natural lipid A from , re-mutant

Kotani, Shunsuke; Takada, Haruhiko; Tsujimoto, M; Ogawa, Tomohiko; Takahashi, I.; Ikeda, Takako; Otsuka, Kojiro; Kasai, Nobuhiko; Mashimo, Jun-ichi; Nagao, Shigeki; Tanaka, Atsuo; Tanaka, Shigeo; Harada, Kazuhiro; Shiba, T.; Kusumoto, Shoichi; Imoto, Masahiro; Yoshimura, Hidetoshi

doi:10.1016/0192-0561(85)90172-9

Cited by 35 publications

(47 citation statements)

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“…Both of these lipid As, the Salmonella-type heptaacylated and E. coli-type hexaacylated forms, had been successfully synthesized chemically in the past (12)(13)(14), and their biological properties had been carefully tested by many investigators (15)(16)(17)(18)(19)(20). The Salmonella-type lipid A had been demonstrated to be very active in mice and rabbits and subsequently had been thought to be a typical lipid A (18 -20) along with that from E. coli.…”

Section: Discussionmentioning

confidence: 99%

“…Escherichia coli-and Salmonella-type lipid A have the most typical lipid A structures so far defined, and both have been synthesized chemically (12)(13)(14) and well characterized biologically by many investigators (15)(16)(17)(18)(19)(20). The chemical structure of these lipid As consists of a hexa-and heptaacylated diglucosamine bearing six and seven fatty acids, respectively, as shown in Fig.…”

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confidence: 99%

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Salmonella-Type Heptaacylated Lipid A Is Inactive and Acts as an Antagonist of Lipopolysaccharide Action on Human Line Cells

Tanamoto

Azumi

2000

The Journal of Immunology

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The stimulation of both THP-1 and U937 human-derived cells by Salmonella lipid A preparations from various strains, as assessed by TNF-α induction and NF-κB activation, was found to be very low (almost inactive) compared with Escherichia coli lipid A, but all of the lipid As exerted strong activity on mouse cells and on Limulus gelation activity. Experiments using chemically synthesized E. coli-type hexaacylated lipid A (506) and Salmonella-type heptaacylated lipid A (516) yielded clearer results. Both lipid A preparations strongly induced TNF-α release and activated NF-κB in mouse peritoneal macrophages and mouse macrophage-like cell line J774-1 and induced Limulus gelation activity, although the activity of the latter was slightly weaker than that of the former. However, 516 was completely inactive on both THP-1 and U937 cells in terms of both induction of TNF-α and NF-κB activation, whereas 506 displayed strong activity on both cells, the same as natural E. coli LPS. In contrast to the action of the lipid A preparations, all the Salmonella LPSs also exhibited full activity on human cells. However, the polysaccharide portion of the LPS neither exhibited TNF-α induction activity on the cells when administered alone or together with lipid A nor inhibited the activity of the LPS. These results suggest that the mechanism of activation by LPS or the recognition of lipid A structure by human and mouse cells may differ. In addition, both 516 and lipid A from Salmonella were found to antagonize the 506 and E. coli LPS action that induced TNF-α release and NF-κB activation in THP-1 cells.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Salmonella-Type Heptaacylated Lipid A Is Inactive and Acts as an Antagonist of Lipopolysaccharide Action on Human Line Cells

Tanamoto

Azumi

2000

The Journal of Immunology

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“…Most of the assay methods were described in previous papers (15,33), and a brief description is given below.…”

Section: Methodsmentioning

confidence: 99%

Possible Existence of a Novel Amphipathic Immunostimulator in the Phenol‐Water Extracts of Mycobacteriaceae

Ikeda-Fujita¹,

Kotani

Tsujimoto³

et al. 1987

Microbiology and Immunology

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“…The death of mice from intoxication was observed for 1 week. The toxicity of LPS was assessed by decrease in body weight in LPS-injected mice as described by Kotani et al (1985). ICR male mice at 4 weeks of age were injected i.p.…”

Section: Toxicity Assaymentioning

confidence: 99%

Anti-tumour activity of low-toxicity lipopolysaccharide of Bordetella pertussis

Ohnishi

Kimura²,

Yamazaki³

et al. 1994

Br J Cancer

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Summary A lipopolysaccharide (BP-LPS) isolated from killed Bordetella pertussis (Tohama strain) was determined to have low toxicity based on the mortality and decrease in body weight of BP-LPS-injected mice. BP-LPS, administered intradermally or intraperitoneally, clearly inhibited the growth of an MM46 murine mammary carcinoma. When compared with a toxic Escherichia coli-derived LPS, BP-LPS displayed excellent anti-tumour activity against MH 134 hepatoma and Meth A fibrosarcoma. As part of a combined chemotherapy/immunotherapy regimen, BP-LPS also seemed to prolong the lifespan of mice inoculated with Lewis lung carcinoma. BP-LPS thus appears to have valuable characteristics as an anti-tumour agent.

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Synthetic lipid A with endotoxic and related biological activities, comparable to a natural lipid A from , re-mutant

Cited by 35 publications

References 0 publications

Salmonella-Type Heptaacylated Lipid A Is Inactive and Acts as an Antagonist of Lipopolysaccharide Action on Human Line Cells

Salmonella-Type Heptaacylated Lipid A Is Inactive and Acts as an Antagonist of Lipopolysaccharide Action on Human Line Cells

Possible Existence of a Novel Amphipathic Immunostimulator in the Phenol‐Water Extracts of Mycobacteriaceae

Anti-tumour activity of low-toxicity lipopolysaccharide of Bordetella pertussis

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