Synthetic Biology Perspectives of Microbial Enzymes and Their Innovative Applications

Shukla, Pratyoosh

doi:10.1007/s12088-019-00819-9

Cited by 26 publications

(18 citation statements)

References 89 publications

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“…From an industrial perspective, wild-type microbial enzymes are usually modified to withstand the harsh and variable reaction conditions, and to satisfy the catalytic needs of a given process ( Shukla, 2019 ). A synthetic biology approach combines a range of molecular and computational tools to generate enzymes that meet industrial expectations.…”

Section: Challenges and Limitationsmentioning

confidence: 99%

“…This can lead to improvements in enzyme activity, stability, and thus reusability, as demonstrated by an immobilized lipase from a Fusarium incarnatum strain, which retained 75% of its degradation activity after 5 cycles when applied to treat waste cooking oil ( Joshi et al, 2019 ). In silico studies and computer simulations bypass lab-scale investigations and are used, for example, to investigate enzyme-substrate interactions and substrate-binding efficiency ( Shukla, 2019 ).…”

Section: Challenges and Limitationsmentioning

confidence: 99%

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Microbial Polyethylene Terephthalate Hydrolases: Current and Future Perspectives

2020

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Plastic has rapidly transformed our world, with many aspects of human life now relying on a variety of plastic materials. Biological plastic degradation, which employs microorganisms and their degradative enzymes, has emerged as one way to address the unforeseen consequences of the waste streams that have resulted from mass plastic production. The focus of this review is microbial hydrolase enzymes which have been found to act on polyethylene terephthalate (PET) plastic. The best characterized examples are discussed together with the use of genomic and protein engineering technologies to obtain PET hydrolase enzymes for different applications. In addition, the obstacles which are currently limiting the development of efficient PET bioprocessing are presented. By continuing to study the possible mechanisms and the structural elements of key enzymes involved in microbial PET hydrolysis, and by assessing the ability of PET hydrolase enzymes to work under practical conditions, this research will help inform large-scale waste management operations. Finally, the contribution of microbial PET hydrolases in creating a potential circular PET economy will be explored. This review combines the current knowledge on enzymatic PET processing with proposed strategies for optimization and use, to help clarify the next steps in addressing pollution by PET and other plastics.

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Section: Challenges and Limitationsmentioning

confidence: 99%

Section: Challenges and Limitationsmentioning

confidence: 99%

Microbial Polyethylene Terephthalate Hydrolases: Current and Future Perspectives

2020

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“…Biocatalysts have been widely used for biotransformation applications such as industrial and environmental sectors [1,2]. The enzymes as cell-free biocatalysts are founded more desirable to use in bioconversion reactions due to their high specificity towards substrate and reaction rate, easy in product separation, and tolerance towards higher substrate concentration, and solvents [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Rhus vernicifera Laccase Immobilization on Magnetic Nanoparticles to Improve Stability and Its Potential Application in Bisphenol A Degradation

et al. 2020

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In the present study, Rhus vernicifera laccase (RvLac) was immobilized through covalent methods on the magnetic nanoparticles. Fe 2 O 3 and Fe 3 O 4 nanoparticles activated by 3-aminopropyltriethoxysilane followed with glutaraldehyde showed maximum immobilization yields and relative activity up to 81.4 and 84.3% at optimum incubation and pH of 18 h and 5.8, respectively. The maximum RvLac loading of 156 mg/g of support was recorded on Fe 2 O 3 nanoparticles. A higher optimum pH and temperature of 4.0 and 45 °C were noted for immobilized enzyme compared to values of 3.5 and 40 °C for free form, respectively. Immobilized RvLac exhibited better relative activity profiles at various pH and temperature ranges. The immobilized enzyme showed up to 16-fold improvement in the thermal stability, when incubated at 60 °C, and retained up to 82.9% of residual activity after ten cycles of reuses. Immobilized RvLac exhibited up to 1.9-fold higher bisphenol A degradation efficiency potential over free enzyme. Previous reports have demonstrated the immobilization of RvLac on nonmagnetic supports. This study has demonstrated that immobilization of RvLac on magnetic nanoparticles is very efficient especially for achieving high loading, better pH and temperature profiles, and thermal-and solvents-stability, high reusability, and higher degradation of bisphenol A.

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“…Enzymes and biocatalysts are being utilized in industries for various purposes, aiming at the production of many sustainable products (Diefenbach et al 2018;Sinha and Shukla 2019). Synthetic biology and other molecular biology tools are widely used to engineer enzymes (Labrou 2010;Platis and Labrou 2008;Chronopoulou et al 2018;Mohammadi et al 2018;Shukla 2019;Mandeep et al 2020;Dixit et al 2020) and fabricate robots (Hägele et al 2016). Robots are perfunctory systems deliberated for precise function.…”

Section: Introductionmentioning

confidence: 99%

Robotics for enzyme technology: innovations and technological perspectives

Dixit

Panchal

Pandey

et al. 2021

Appl Microbiol Biotechnol

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The use of robotics in the life science sector has created a considerable and significant impact on a wide range of research areas, including enzyme technology due to their immense applications in enzyme and microbial engineering as an indispensable tool in high-throughput screening applications. Scientists are experiencing the advanced applications of various biological robots (nanobots), fabricated based on bottom-up or top-down approaches for making nanotechnology scaffolds. Nanobots and enzyme-powered nanomotors are particularly attractive because they are self-propelled vehicles, which consume biocompatible fuels. These smart nanostructures are widely used as drug delivery systems for the efficient treatment of various diseases. This review gives insights into the escalating necessity of robotics and nanobots and their ever-widening applications in enzyme technology, including biofuel production and biomedical applications. It also offers brief insights into high-throughput robotic platforms that are currently being used in enzyme screening applications for monitoring and control of microbial growth conditions. Key points• Robotics and their applications in biotechnology are highlighted.• Robotics for high-throughput enzyme screening and microbial engineering are described.• Nanobots and enzyme-powered nanomotors as controllable drug delivery systems are reviewed.

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Synthetic Biology Perspectives of Microbial Enzymes and Their Innovative Applications

Cited by 26 publications

References 89 publications

Microbial Polyethylene Terephthalate Hydrolases: Current and Future Perspectives

Microbial Polyethylene Terephthalate Hydrolases: Current and Future Perspectives

Rhus vernicifera Laccase Immobilization on Magnetic Nanoparticles to Improve Stability and Its Potential Application in Bisphenol A Degradation

Robotics for enzyme technology: innovations and technological perspectives

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