Synthetic biology for fibers, adhesives, and active camouflage materials in protection and aerospace

Roberts, Aled D.; Finnigan, William; Wolde-Michael, Emmanuel; Kelly, Paul P.; Blaker, Jonny J.; Hay, Sam; Breitling, Rainer; Takano, Eriko; Scrutton, Nigel S.

doi:10.1557/mrc.2019.35

Cited by 24 publications

(34 citation statements)

References 167 publications

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“…In concert with this, its extremely fast growth rate assists in genetically engineering this organism [7,12,13]. Third, its tolerance to high salt-in some media, up to 30 g·L −1 (~0.51 M) with an optimum at 15 g·L −1 (~0.26 M)-coupled to fast growth could help maintain axenic fermentation conditions without expensive antibiotic techniques; high salt tolerance has been referenced as an advantage for other emergent chassis such as Halomonas which grows in 60 g·L −1 NaCl [7,14,15]. Illustrating the flexibility of V. natriegens, if non-corrosive fermenters are not available to accommodate high-salt growth, it can still be grown with a low chlorine-based media [7,14,15].…”

Section: Introductionmentioning

confidence: 99%

Exploiting the Feedstock Flexibility of the Emergent Synthetic Biology Chassis Vibrio natriegens for Engineered Natural Product Production

et al. 2019

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A recent goal of synthetic biology has been to identify new chassis that provide benefits lacking in model organisms. Vibrio natriegens is a marine Gram-negative bacterium which is an emergent synthetic biology chassis with inherent benefits: An extremely fast growth rate, genetic tractability, and the ability to grow on a variety of carbon sources ("feedstock flexibility"). Given these inherent benefits, we sought to determine its potential to heterologously produce natural products, and chose beta-carotene and violacein as test cases. For beta-carotene production, we expressed the beta-carotene biosynthetic pathway from the sister marine bacterium Vibrio campbellii, as well as the mevalonate biosynthetic pathway from the Gram-positive bacterium Lactobacillus acidophilus to improve precursor abundance. Violacein was produced by expressing a biosynthetic gene cluster derived from Chromobacterium violaceum. Not only was V. natriegens able to heterologously produce these compounds in rich media, illustrating its promise as a new chassis for small molecule drug production, but it also did so in minimal media using a variety of feedstocks. The ability for V. natriegens to produce natural products with multiple industrially-relevant feedstocks argues for continued investigations into the production of more complex natural products in this chassis.

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Section: Introductionmentioning

confidence: 99%

Exploiting the Feedstock Flexibility of the Emergent Synthetic Biology Chassis Vibrio natriegens for Engineered Natural Product Production

et al. 2019

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“…[32][33][34][35] Finally, the use of genetically engineered silks such as recombinant spider silk could tune the mechanical and textural properties of the silk, or introduce chemical functionality such as additional lysine groups (-NH2) to neutralise toxic VOCs or other substances. [36]…”

Section: Resultsmentioning

confidence: 99%

Hierarchically Porous Silk/Activated-Carbon Composite Fibers for Adsorption and Repellence of Volatile Organic Compounds

Roberts¹,

Lee²,

Magaz³

et al. 2020

Preprint

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Fabrics comprised of porous fibers could provide effective passive protection against chemical and biological (CB) threats whilst maintaining high air permeability (breathability). Here, we fabricate hierarchically porous fibers consisting of regenerated silk fibroin (RSF) and activated-carbon (AC) prepared through two fiber spinning techniques in combination with ice-templating – namely cryogenic solution blow spinning (Cryo-SBS) and cryogenic wet-spinning (Cryo-WS). The Cryo-WS RSF fibers had exceptionally small macropores (as low as 0.1 µm) and high specific surface areas (SSAs) of up to 79 m2 g-1. The incorporation of AC could further increase the SSA to 210 m2 g-1 (25 wt. % loading) whilst also increasing adsorption capacity for volatile organic compounds (VOCs).

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“…Porous RSF fibres such as these, loaded with other functional substances rather than AC, could have applications in a range of fields, particularly tissue regeneration and controlled drug delivery [32][33][34][35]. Finally, the use of genetically engineered silks such as recombinant spider silk could tune the mechanical and textural properties of the silk, or introduce chemical functionality such as additional lysine groups (-NH 2 ) to neutralise toxic VOCs or other substances [36].…”

Section: Discussionmentioning

confidence: 99%

Hierarchically Porous Silk/Activated-Carbon Composite Fibres for Adsorption and Repellence of Volatile Organic Compounds

et al. 2020

Self Cite

View full text Add to dashboard Cite

Fabrics comprised of porous fibres could provide effective passive protection against chemical and biological (CB) threats whilst maintaining high air permeability (breathability). Here, we fabricate hierarchically porous fibres consisting of regenerated silk fibroin (RSF) and activated-carbon (AC) prepared through two fibre spinning techniques in combination with ice-templating—namely cryogenic solution blow spinning (Cryo-SBS) and cryogenic wet-spinning (Cryo-WS). The Cryo-WS RSF fibres had exceptionally small macropores (as low as 0.1 µm) and high specific surface areas (SSAs) of up to 79 m2·g−1. The incorporation of AC could further increase the SSA to 210 m2·g−1 (25 wt.% loading) whilst also increasing adsorption capacity for volatile organic compounds (VOCs).

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Synthetic biology for fibers, adhesives, and active camouflage materials in protection and aerospace

Abstract: Abstract

Cited by 24 publications

References 167 publications

Exploiting the Feedstock Flexibility of the Emergent Synthetic Biology Chassis Vibrio natriegens for Engineered Natural Product Production

Exploiting the Feedstock Flexibility of the Emergent Synthetic Biology Chassis Vibrio natriegens for Engineered Natural Product Production

Hierarchically Porous Silk/Activated-Carbon Composite Fibers for Adsorption and Repellence of Volatile Organic Compounds

Hierarchically Porous Silk/Activated-Carbon Composite Fibres for Adsorption and Repellence of Volatile Organic Compounds

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