Synthetic Biology-Driven Microbial Production of Resveratrol: Advances and Perspectives

Abstract

Resveratrol, a bioactive natural product found in many plants, is a secondary metabolite and has attracted much attention in the medicine and health care products fields due to its remarkable biological activities including anti-cancer, anti-oxidation, anti-aging, anti-inflammation, neuroprotection and anti-glycation. However, traditional chemical synthesis and plant extraction methods are impractical for industrial resveratrol production because of low yield, toxic chemical solvents and environmental pollution during the production process. Recently, the biosynthesis of resveratrol by constructing microbial cell factories has attracted much attention, because it provides a safe and efficient route for the resveratrol production. This review discusses the physiological functions and market applications of resveratrol. In addition, recent significant biotechnology advances in resveratrol biosynthesis are systematically summarized. Furthermore, we discuss the current challenges and future prospects for strain development for large-scale resveratrol production at an industrial level.

An assessment of the rescue action of resveratrol in parkin loss of function-induced oxidative stress in Drosophila melanogaster

“…Since oxidative stress is linked with the pathogenesis of PD 36 , any agent that can maintain the redox and oxidative stress-antioxidant balance might be a promising therapeutic drug for PD 37 . Resveratrol (3,5,4'-trihydroxystilbene) is a naturally existing polyphenolic compound found in nuts and fruits such grapes 38,39 . It has been reported to possess both anti-inflammatory and antioxidative properties 40 .…”

An image-processing-based technique for detecting quantitative fluorescence in plant cells

“…We rst demonstrated that ACFVA could accurately measure many different biologically important features of suspensions cells using several plant cell types, including live Arabidopsis protoplasts and Dunaliella cells because these cells are particularly challenging to identify by automated image analysis (Lobet et al, 2013;Sekulska-Nalewajko et al, 2016), and they enable rapid chemical substance detection such as pollutants in the environment or generation of large quantities of active ingredients such as Plant-derived therapeutic proteins using living cell after genetic modi cation (Feng et al, 2022;Liu and Stewart Jr, 2015). Using the basic cell-culture methods described previously (Hosseini Tafreshi and Shariati, 2009; Yoo et al, 2007), we prepared Arabidopsis protoplasts and Dunaliella cells for experiments shown in Figs.…”

“…By contrast, automated analysis rapidly produces consistent, quantitative measures for every image (Stöter et al, 2013). This is very important for synthetic biology whose emphasis was engineering and conformity to obtain systems-level conclusions from the quantitative measures a large number of features even features undetectable by eye for every image (Feng et al, 2022;Schweizer, 1980).…”

“…With the development of modern technology, more and more problems such as cleaning hazardous waste in inaccessible places (Cases and Lorenzo, 2005),sensing chemicals dynamicly and responding accordingly (Antunes et al, 2006;Bowen et al, 2008) and producing clean fuel (Savage et al, 2008) need some novel and convenient approaches for solving these complex problems. Synthetic biology has the potential to combines the investigative nature of biology with the constructive nature of engineering which can meet the above requirement (Feng et al, 2022;Purnick and Weiss, 2009). Especially, when plant cells have applied into creation and perfection of genetic devices and small modules which would bring signi cant advances in synthetic biology (Brophy, 2022;Zhu et al, 2021).…”

Valorisation of wine wastes by de novo biosynthesis of resveratrol using a recombinant xylose-consuming industrial Saccharomyces cerevisiae strain

“…Furthermore, because malonyl-CoA is primarily employed as an important intermediary in fatty acid biosynthesis to maintain cell development, only a small amount of malonyl-CoA is available for resveratrol biosynthesis, which is a significant hurdle in resveratrol production. 40 Malonyl-CoA is converted from acetyl-CoA in a reaction catalysed by acetyl-CoA carboxylase (encoded by ACC1 ), which originated in the yeast cell from acetate. In its turn, acetate is derived from acetaldehyde that results from pyruvate or ethanol consumption, which can help to balance the supply of malonyl-CoA in the yeast cell (Fig.…”