Synthetic Biology: Current State and Applications

Vasilev, Ruslan; Chernikovich, V. Yu.; Evteeva, Martha A.; Sakharov, D. A.; Патрушев, М. В.

doi:10.3103/s0891416821010079

Cited by 1 publication

(1 citation statement)

References 67 publications

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“…Although increasing numbers of TFs are repurposed for synthetic biology year-by-year, the field is still running short of diverse, orthogonal TF sets that are required for increasing circuit complexity [3][4][5][6][7][8]. Expanding this toolkit through engineering TFs with different properties and orthogonal activities is, therefore, a topic of current focus [3,7,[9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Reducing metabolic burden in the PACEmid evolver system by remastering high‐copy phagemid vectors

Davenport

Tica

Isalan

2022

Engineering Biology

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Orthogonal or non‐cross‐reacting transcription factors are used in synthetic biology as components of genetic circuits. Brödel et al. (2016) engineered 12 such cIλ transcription factor variants using a directed evolution ‘PACEmid’ system. The variants operate as dual activator/repressors and expand gene circuit construction possibilities. However, the high‐copy phagemid vectors carrying the cIλ variants imposed high metabolic burden upon cells. Here, the authors ‘remaster’ the phagemid backbones to relieve their burden substantially, exhibited by a recovery in Escherichia coli growth. The remastered phagemids' ability to function within the PACEmid evolver system is maintained, as is the cIλ transcription factors' activity within these vectors. The low‐burden phagemid versions are more suitable for use in PACEmid experiments and synthetic gene circuits; the authors have, therefore, replaced the original high‐burden phagemids on the Addgene repository. The authors’ work emphasises the importance of understanding metabolic burden and incorporating it into design steps in future synthetic biology ventures.

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