Synthetic Biology and Metabolic Engineering Approaches To Produce Biofuels

Metabolic Engineering for Advanced Biofuels Production and Recent Advances Toward Commercialization

“…Moreover, slow culturing rates and spore formation have rendered them unideal for commercial-scale alcohol production. [8,9] A substantial number of studies have since been performed in E. coli and S. cerevisiae that enhanced the variety and productivity of various linear and branched-chain alcohols. Various optimization strategies in these organisms revolve around two core pathways, the acetyl-CoA dependent ABE pathway and the keto acid pathway derived from amino acid biosynthesis ( Figure 1).…”

Application à la biologie synthétique des méthodes et outils de CAO de la microélectronique

“…Le champ d'application de la biologie synthétique est très vaste. Il inclut la mise au point de nouvelles thé-rapies [3] et de thérapies ciblées [4], la réduction des coûts de synthèse de certains médicaments [5,6], le diagnostic in vivo [7,8], la conception de nouvelles fonctions traitant directement des signaux biologiques [9][10][11][12], la mise au point de biocapteurs [3,9,[13][14][15], l'agriculture et l'agronomie [16] ou encore l'environnement [17,18]. fonctionnement du système peut être résumé par les propositions logiques suivantes :…”

Can Microbially Derived Advanced Biofuels Ever Compete with Conventional Bioethanol? A Critical Review

“…Despite a relatively higher success rate in the engineering of Clostridia pathway and amino acid biosynthesis pathway in E. coli as compared to S. cerevisiae (Rabinovitch-Deere et al 2013), overcoming E. coli solvent toxicity presents a continued challenge (Table 3). It is apparent that E. coli can tolerate solvent concentrations in the vicinity of 1 to 2%.…”

“…S. cerevisiae has been industrially proven for bulk production in both the ethanol and food industries. While it can be argued that genetic engineering efforts with E. coli have been relatively more successful than S. cerevisiae (Rabinovitch-Deere et al 2013), E. coli has yet to be used at a large scale. A successful example of smaller scale industrial applications of genetically engineered E. coli is the production of 1,3 propanediol and polyhydroxybutyrate (PHA), under development by Metabolix and ADM. High value "niche" industrial applications of genetically engineered E. coli include glycosylated pharmaceuticals, antibiotics, and recombinant protein production.…”