Synthetic biology: advancing biological frontiers by building synthetic systems

Chen, Yvonne Yu-Hsuan; Galloway, Kate E.; Smolke, Christina D.

doi:10.1186/gb-2012-13-2-240

Cited by 70 publications

(45 citation statements)

References 92 publications

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“…Revista da Biologia (2015) 14(1):33-39 DOI: 10.7594/revbio.14.01.05 mas das técnicas utilizadas no experimento; programas de computador auxiliaram, in silico, na fragmentação do genoma em 1.100 partes aproximadamente iguais, cada uma contendo cerca de 1.080 pb (Gibson et al, 2010;Chen et al, 2012 (Bouzarc, 2010;Gibson et al, 2010).…”

Section: Biologia Sintéticaunclassified

“…Destaca-se a utilização de algas marinhas para a detecção de poluentes marinhos e sua remoção pela captação do dióxido de carbono, em casos de desastres naturais ocorridos por vazamento de petróleo em navios petroleiros, bem como a biorremediação, em contaminações de recursos hídricos por metais pesados (Chen et al, 2012).…”

Section: Biopirataria Digital E Bioterrorismounclassified

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Biologia Sintética: possibilidades e desafios

Paulillo¹

2015

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Resumo.A biologia sintética, principalmente por causa das técnicas desenvolvidas, adquiriu atenção distinta entre cientistas, ambientalistas, políticos, bem como a sociedade, após o feito mais significativo até então: criação do primeiro organismo vivo controlado por um genoma sintético, isto é, a bactéria Mycoplasma mycoides JCVI-syn1.0. Portanto, o foco deste estudo foi realizar uma revisão da literatura sobre a biologia sintética, com o objetivo de identificar as possibilidades e os desafios existentes. Assim, os dados foram coletados a partir de publicações indexadas em bases de dados eletrônicas e a análise dos dados revelou que existem possibilidades de aplicações práticas para a indústria, meio ambiente e saúde humana, mas estas serão acompanhadas por desafios significativos. Palavras-chave. Genoma sintético; Mycoplasma mycoides; tecnologias emergentes.Abstract. Synthetic Biology, mainly because of the techniques developed, has acquired distinct attention among scientists, environmentalists, political as well as the society after the more significant accomplishment until then: creation of the first living organism controlled by a synthetic genome; i.e., the bacterium Mycoplasma mycoides JCVI-syn1.0. Therefore, the focus of this study was to undertake a review of the literature on synthetic biology with the aim of identifying existing possibilities and challenges. Thus, the data were collected from indexed publications in electronic databases; the data analysis revealed that there are possibilities for practical applications for industry, environment, and human health; but these possibilities will be accompanied by significant challenges. Keywords. Emerging technologies; Mycoplasma mycoides; synthetic genome. *Contato: jossanborba@gmail.com IntroduçãoDesde 2003, cientistas desenvolvem uma nova tecnologia denominada biologia sintética, ou synbio, como sendo o limite seguinte da biociência (EASAC, 2011). Tratando-se de uma abordagem sistêmica, interdisciplinar e multiprofissional, principalmente por causa das técnicas desenvolvidas, adquiriu atenção distinta entre cientistas, ambientalistas, políticos, bem como a sociedade (Schneider, 2007).Em 2010, por meio de uma publicação dos cientistas do J. Craig Venter Institute (JCVI -instituto fundado pelo bioquímico, geneticista e empresário norte-americano John Craig Venter), na revista Science, foi descrito o feito mais significativo até então: criação do primeiro organismo vivo controlado por um genoma sintético, isto é, a bactéria Mycoplasma mycoides JCVI-syn1.0. (Gibson et al., 2010). Tal descrição apresentou-se como uma promissora e alarmante preparação para o objetivo maior de transformar microrganismos naturais (bactérias, leveduras, algas e vírus) em sintéticos (com genomas sintéticos) para realizarem funções predefinidas e específicas (Centro Ecológico, 2009.Após o feito de 2010, relevantes ponderações surgiram sobre o desenvolvimento de técnicas ainda pouco compreendidas, ou seja, se estas apresentariam mais resultados positivos ou negativos (ETC Group, ...

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Section: Biologia Sintéticaunclassified

Section: Biopirataria Digital E Bioterrorismounclassified

Biologia Sintética: possibilidades e desafios

Paulillo¹

2015

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“…As opposed to digital circuits, analog circuits create graded output curves in response to input signals [93, 94]. During bioprocesses, externally applied analog inputs can be used to tune pathway enzyme levels over an entire microbial population.…”

Section: Analog Circuitsmentioning

confidence: 99%

Digital and analog gene circuits for biotechnology

Roquet

2014

Biotechnology Journal

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Biotechnology offers the promise of valuable chemical production via microbial processing of renewable and inexpensive substrates. Thus far, static metabolic engineering strategies have enabled this field to advance industrial applications. However, the industrial scaling of statically engineered microbes inevitably creates inefficiencies due to variable conditions present in large-scale microbial cultures. Synthetic gene circuits that dynamically sense and regulate different molecules can resolve this issue by enabling cells to continuously adapt to variable conditions. These circuits also have the potential to enable next-generation production programs capable of autonomous transitioning between steps in a bioprocess. Here, we review the design and application of two main classes of dynamic gene circuits, digital and analog, for biotechnology. Within the context of these classes, we also discuss the potential benefits of digital-analog interconversion, memory, and multi-signal integration. Though synthetic gene circuits have largely been applied for cellular computation to date, we envision that utilizing them in biotechnology will enhance the efficiency and scope of biochemical production with living cells.

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“…The engineering of biological systems to regulate cell fate requires precise control over gene circuit performance (11, 12) and the ability to interface with key decision-making pathways (13). Advances in synthetic biology may facilitate the design of sophisticated genetic circuits capable of sensing and actuating changes in native signaling networks (14). …”

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confidence: 99%

Dynamically Reshaping Signaling Networks to Program Cell Fate via Genetic Controllers

Galloway

Franco

Smolke

2013

Science

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Engineering of cell fate through synthetic gene circuits requires methods to precisely implement control around native decision-making pathways, and offers the potential to direct cell processes. We demonstrate a class of genetic control systems, molecular network diverters, that interface with a native signaling pathway to route cells to divergent fates in response to environmental signals without modification of native genetic material. A method for identifying control points within natural networks is described that enables the construction of synthetic control systems that activate or attenuate native pathways to direct cell fate. We integrate opposing genetic programs by developing network architectures for reduced antagonism and demonstrate rational tuning of performance. Extension of these control strategies to mammalian systems should facilitate the engineering of complex cellular signaling systems.

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Synthetic biology: advancing biological frontiers by building synthetic systems

Abstract: Advances in synthetic biology are contributing to diverse research areas, from basic biology to biomanufacturing and disease therapy. We discuss the theoretical foundation, applications, and potential of this emerging field.

Cited by 70 publications

References 92 publications

Biologia Sintética: possibilidades e desafios

Biologia Sintética: possibilidades e desafios

Digital and analog gene circuits for biotechnology

Dynamically Reshaping Signaling Networks to Program Cell Fate via Genetic Controllers

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