Synthetic Approaches for Total Chemical Synthesis of Proteins and Protein‐Like Macromolecules of Branched Architecture

Papas, Serafim; Strongylis, Constantinos; Tsikaris, Vassilios

doi:10.1002/chin.200734255

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“…Physically crosslinked, solid injectable hydrogels can be developed from peptides, proteins, or polymers. With rapid advances in peptide, protein, polymer, and protein–polymer conjugate synthesis and materials development, rheological characterization has become an increasingly important tool to obtain more information about the viscoelastic and flow properties of hydrogels based on peptides, proteins, and polymers. Common rheological studies conducted on hydrogel materials include measurement of shear storage modulus (G′) (qualitatively the material stiffness), loss modulus (G″) (qualitatively the material liquid‐like flow properties), and loss factor tan ( δ ) (the ratio of liquid‐like behavior to solid‐like behavior), measured as functions of time, oscillatory frequency, and oscillatory strain.…”

Section: Introductionmentioning

confidence: 99%

Rheology of peptide‐ and protein‐based physical hydrogels: Are everyday measurements just scratching the surface?

Sathaye

Mbi

Sönmez

et al. 2014

WIREs Nanomed Nanobiotechnol

113

103

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Rheological characterization of physically crosslinked peptide- and protein-based hydrogels is widely reported in the literature. In this review, we focus on solid injectable hydrogels, which are commonly referred to as 'shear-thinning and rehealing' materials. This class of what sometimes also are called 'yield-stress' materials holds exciting promise for biomedical applications that require well-defined morphological and mechanical properties after delivery to a desired site through a shearing process (e.g., syringe or catheter injection). In addition to the review of recent studies using common rheometric measurements on peptide- and protein-based, physically crosslinked hydrogels, we provide experimentally obtained visual evidence, using a rheo-confocal microscope, of the fracture and subsequent flow of physically crosslinked β-hairpin peptide hydrogels under steady-state shear mimicking commonly conducted experimental conditions using bench-top rheometers. The observed fracture demonstrates that the supposed bulk shear-thinning and rehealing behavior of physical gels can be limited to the yielding of a hydrogel layer close to the shearing surface with the bulk of the hydrogel below experiencing negligible shear. We suggest some measures to be taken while acquiring and interpreting data using bench-top rheometers with a particular focus on physical hydrogels. In particular, the use of confocal-rheometer assembly is intended to inspire studies on yielding behavior of hydrogels perceived as shear-thinning and rehealing materials. A deeper insight into their yielding behavior will lead to the development of yield-stress, injectable, solid biomaterials, and hopefully inspire the design of new shear-thinning and rehealing hydrogels and more thorough physical characterization of such systems. Finally, more examples of bulk fracture in some physical hydrogels based on peptides and proteins are explored in the light of their behavior as yield-stress materials.

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Section: Introductionmentioning

confidence: 99%

Rheology of peptide‐ and protein‐based physical hydrogels: Are everyday measurements just scratching the surface?

Sathaye

Mbi

Sönmez

et al. 2014

WIREs Nanomed Nanobiotechnol

113

103

View full text Add to dashboard Cite

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Synthetic Approaches for Total Chemical Synthesis of Proteins and Protein‐Like Macromolecules of Branched Architecture

Abstract: ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.

Cited by 1 publication

References 1 publication

Rheology of peptide‐ and protein‐based physical hydrogels: Are everyday measurements just scratching the surface?

Rheology of peptide‐ and protein‐based physical hydrogels: Are everyday measurements just scratching the surface?

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