Synthethic peptide used to develop antibodies for detection of polyhedrin from monodon baculovirus (MBV)

Satidkanitkul, Attaphon; Sithigorngul, Paisarn; Sang-oum, Wiwat; Rukpratanporn, Sombat; Sriurairatana, Siriporn; Withayachumnankul, Boonsirm; Flegel, Timothy W.

doi:10.3354/dao065079

Cited by 12 publications

(5 citation statements)

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“…Polyhedrin là loại protein tinh thể đặc trưng của baculovirus thuộc họ Baculoviridae, có vai trò là chất nền bảo vệ vỏ của virion, giữ cho hạt virus ổn định trong điều kiện biến đổi của môi trường, tránh được sự phân giải của các enzym từ gan tuỵ. Polyhedrin cũng là phần quyết định kháng nguyên khi virus xâm nhiễm vào cơ thể vật chủ và tạo đáp ứng miễn dịch kháng lại sự lây nhiễm của MBV (Satidkanitkul et al, 2005). Việc xác định trình tự gen polhed có ý nghĩa lớn cả về nghiên cứu cơ bản để tìm hiểu sự biến đổi của virus theo các vùng địa lý và về việc ứng dụng trong chẩn đoán đặc hiệu.…”

Section: A B Cunclassified

Sequence analysis of thegene encoding polyhedrin of monodon baculovirus (MBV) infected in penaeid shrimp (Penaeus monodon) in Vietnam

Quyền

Kháng

2018

Vietnam J. Biotechnol.

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Monodon Baculovirus (MBV) is a causative agent in farmed shrimp. It is a double-stranded DNA virus with genome size of 119.638 bp. MBV virions are free in the nuclei or covered by the occlusion bodies(Obs) constructed by polyhedrin matrix proteins of MBV. OBs serve to protect the occluded virions from the outside environment for extended periods until they are ingested by insect larvae. Polyhedrin is a type of protein covering the viruswith size from 17 to 23 nm, as well as a surface antigen used for Baculovirus detection. Sequence analysis of the gene coding for polyhedrin (polhed) showed that their open reading frame (ORF) was 1,371 bp long and coding for a polypeptide of 457 amino acids.The alignment of nucleotide sequencesof all polyhedrin genes including those of MBV isolated in Vietnam and from GenBank showed that polhed from Vietnam has 97%, 97% and 94% sequence identity compared to that of Thailand, Taiwan and India, respectively. Amino acid analysis revealed that the insertions occur at the sites 72-73 with 4 aa (p.72_73insNEPM) and at 81-82 with 7 aa (p.81_82ins PYHNSPN) in the MBV isolated in Vietnam in comparison with that of Thailand. In addition, we also observed other amino acid substitutions in the protein sequence as: p.Thr55Gly, p.Glu63Asp, p.Asn72Asp, p.Glu92Gly, p.Met93Leu, p.Lys108Asnand p.Lys113Asn. These amino acid changes may affect the protein structure and reduce the specificity of the antigen. Therefore, the foreign kits for MBV diagnosis may have less specificity when using for inland MBV strains detection. The result of our research suggests that MBV which cause diseases in Vietnamese shrimps have notable genetic deviation compared to that of Thailand’s and other countries’ in the world.

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Section: A B Cunclassified

Sequence analysis of thegene encoding polyhedrin of monodon baculovirus (MBV) infected in penaeid shrimp (Penaeus monodon) in Vietnam

Quyền

Kháng

2018

Vietnam J. Biotechnol.

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“…Polymerase chain reaction (PCR) is a rapid method for viral detection. However, experience has shown that the Taiwanese primers do not work with MBV from Thailand and Australia [2,28,29]. Properties of the virus summarized in Table 1.…”

Section: Diagnosticmentioning

confidence: 99%

A Short Review on Infectious Viruses in Cultural Shrimps (Penaeidae Family)

Ganjoor¹

2015

Fish Aquac J

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A major constraint limiting the shrimp production is diseases. Shrimp aquaculture is an important industry in many countries especially Southeast Asia and Iran. In cultured pond, the shrimp may be infected with several pathogens such as several viruses. There are at least six lethal viruses affecting penaeid shrimps production in the world especially Southeast Asia and Thailand. However, known viral pathogen in shrimp is about 20. They have been identified from 1970. Incidence of infection in artificial condition is more than nature. The 6 viruses are very important and they cause serious problem for shrimp cultivation and economic losses. They are consisting of HPV, IHHNV, MBV, TSV, WSSV and YHV. Two of them are highly pathogenic and lethal in shrimp such as WSSV and TSV. Shrimp aquaculture is a successful activity. Despite this success, annual production decreased in the latter because of widespread epidemics (epizootics) caused by new viral pathogens. Molecular diagnostic methods such as PCR are tools to detection viral diseases in shrimp in many parts of the world. Pathological methods and electron microscopy are good tools to detection viral disease especially at the first outbreak. Sanitary methods are the best way to control and prevention of viral diseases.

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“…ELISA can be used to detect MBV in tissue homogenates using polyclonal antibodies developed against the OB [43]. Immunological diagnosis using polyclonal antibodies or monoclonal antibodies raised against MBV polyhedrin has also been reported [8,83,86].…”

Section: Diagnosismentioning

confidence: 99%

Monodon Baculovirus of Shrimp

Rajendran

Makesh

Karunasagar³

2012

Indian J. Virol.

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Among the viruses infecting penaeid shrimp, monodon-type baculovirus (MBV) otherwise known as Penaeus monodon singly enveloped nuclear polyhedrosis virus (PmSNPV), is one of the widely reported and well described viruses. It is a rod-shaped, enveloped, double-stranded DNA virus, and considered till recently, as the type A baculovirus. Besides MBV, two strains of SNPV are reported-plebejus baculovirus and bennettae baculovirus. MBV was reported to be originated from Taiwan and has wide geographic distribution and is reported to be enzootic in wild penaeids of the Indo-pacific coasts of Asia. The virus also has diverse host-range including a variety of cultured and captured shrimp species and freshwater prawn, Macrobrachium rosenbergii. MBV has been reported in all life stages of P. monodon with late larval, postlarval and young juvenile as the most susceptible stages/ages. However, MBV has not been documented in early larval stages. Although MBV has been reported to be tolerated well by shrimp, the infection has been attributed to decreased productivity. The target organs or tissues of MBV are the hepatopancreatic tubules and duct epithelium of postlarvae, juveniles and adults, and the anterior midgut epithelium of very young postlarvae. The prominent clinical sign of infection is the presence of multiple spherical inclusion bodies in the hepatopancreas and midgut epithelial cells. The major mode of transmission of the virus is horizontal through oral exposure to occlusion bodies, contaminated tissues or fomites. Minor morphometric variation of the virus has been reported among different isolates. The rod-shaped enveloped virus particles range from 265-324 nm in length and 42-77 nm in diameter. Although complete genome sequence of MBV is not available, nucleic acid of MBV is circular, double-stranded DNA with a genome size ranging from 80 to 160 kbp. The virus codes for a 53 kDa major polyhedrin polypeptide and two minor 47 and 49 kDa polypeptides. A variety of diagnostic tools have been reported for this virus including real-time PCR and LAMP-based detection. Taxonomic position is still uncertain and International Committee on Taxonomy of Viruses lists MBV as a tentative species named PemoNPV in the genus Nucleopolyhedrovirus. However, according to the latest genomic information on the virus, it has been suggested to create a new group of non-occluded bacilliform viruses called nudiviruses with MBV as one of the members. The aim of the current work is to describe the knowledge on the status, distribution and host-range, pathology, transmission, virus structure and morphogenesis, genomic characteristics, diagnosis and the latest taxonomic position of MBV.

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Synthethic peptide used to develop antibodies for detection of polyhedrin from monodon baculovirus (MBV)

Cited by 12 publications

References 11 publications

Sequence analysis of thegene encoding polyhedrin of monodon baculovirus (MBV) infected in penaeid shrimp (Penaeus monodon) in Vietnam

Sequence analysis of thegene encoding polyhedrin of monodon baculovirus (MBV) infected in penaeid shrimp (Penaeus monodon) in Vietnam

A Short Review on Infectious Viruses in Cultural Shrimps (Penaeidae Family)

Monodon Baculovirus of Shrimp

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