We present a new efficient method for obtaining multicolor digital Fresnel holograms of three-dimensional (3-D) objects emitting incoherent fluorescent light. For each emitted fluorescent light wavelength, a single monochromatic digital modified Fresnel hologram of the 3-D scene is generated. Using a macrolens array, only nine projections of the 3-D scene are captured in a single camera shot and are then digitally processed to yield the hologram. The 3-D reconstructed images from all the monochromatic holograms are composed into a single multicolor image of the 3-D scene. Fluorescence radiation is useful for various applications in the fields of medical imaging, microscopy, biology, and chemistry. High sensitivity, low background noise, and thus good detectability and localization [1] are only some of the advantages of fluorescence imaging that have encouraged the foundation of fluorescence three-dimensional (3-D) imaging methods. Holography is considered a reliable 3-D imaging technique, capable of creating an authentic illusion of 3-D objects without special observation devices. Moreover, digital holograms can store 3-D information in a very dense and encrypted way. However, classic holographic methods demand wave interference, requiring meticulous stability of the entire optical system, as well as a high intensity and long coherence length of the laser used.New techniques for generating holograms of 3-D objects emitting fluorescent (incoherent) light have the advantages of fluorescence over regular illumination and yet eliminate most of the disadvantages of conventional holography. Fluorescence scanning holography [2] is an example of such a technique. However, this method is relatively slow and complicated because of the mechanical scanning required. Multicolor Fresnel incoherent correlation holography [3] is a different technique that does not use mechanical scanning. However, three different holograms for each emitted wavelength should be recorded for generating the final hologram by multicolor Fresnel incoherent correlation holography, in contrast to the method proposed herein.By using multiple viewpoint projection (MVP) holography [4,5], it is possible to generate digital and optical holograms of 3-D, real-existing objects illuminated by incoherent light. These holograms are generated by first acquiring MVPs of the 3-D scene from various perspectives and then digitally processing the MVPs to yield the hologram of the scene.For acquiring these MVPs, the digital camera can be shifted mechanically [4,5]. However, this process is slow and inadequate when the objects move faster than the scanning camera. Instead, it is possible to utilize a microlens array for acquiring the entire set of MVPs in a single camera shot [6]. However, for a high-resolution hologram, an array of many microlenses is needed. Since the total size of the microlens array is limited, the diameter of each microlens is small, and thus each projection is imaged with a relatively poor resolution.To obtain MVP holograms of moving objects with...