Synthesize Analysis of the IFN-γ and Immune Infiltrates of m6A RNA Methylation Regulators in Human Skin Cutaneous Melanoma

Abstract

Background: SKCM is a major common cancer with highly mortality and morbidity, causing about 72% of deaths in skin carcinoma. In recent years, more scholars have selected one or two m6A regulatory factors to explore the abnormal expression and potential mechanism of m6A in tumorigenesis and development. So as to find the relevant biomarkers in the whole tumorigenesis process.Methods: In current study, we used public transcriptome datasets from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) and Genotype-Tissue Expression (GTEx) to investigate the relationships between N6-methyl adenosine(m6A) regulators genes and Skin cutaneous melanoma (SKCM). Then, SKCM patients were grouped based on the cluster analysis of m6A regulators expression. Compared the relationship between Interferon (IFN)-γ and immune infiltrates. Results: Based on the survival curves of subgroups, we selected 20 potential predictive m6A-related genes were overexpressed in SKCM. And based on the typing results, we got the 15 differential expression genes between the three groups. Four of them (CYP2U1, SEMA6A, GRIA2, and TSPAN13) were selected in Cox regression, which were significant expression in overall survival(p<0.05). Interferon (IFN)-γ is a central cytokine, which effecting immune-provoking and recognizing transformed cells in anti-tumour immunity. Conclusions: To investigate the correlation between IFN-γ and SKCM. We have identified that IFN-γ is a potential factor for cancer therapy, and affecting expression pathways of SKCM. Among them, IFN-γ and WTAP were the most positive correlation. These sights suggest that IFN-γ can potentially be utilized for immune pathway such as melanoma skin cancer.