Synthesis, structural characterization and in vitro pharmacological properties of betanin-encapsulated chitosan nanoparticles

Shafqat, Omayya; Rehman, Zartasha; Shah, Muhammad Musaddiq; Ali, Sajid; Jabeen, Zahra; Rehman, Sidra

doi:10.1016/j.cbi.2022.110291

Cited by 8 publications

(7 citation statements)

References 34 publications

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“…The polydispersity index quantifies the degree of homogeneity in particle size. A smaller PDI value signifies a more homogeneous nanoparticle size distribution [31]. PDI values that are less than 0.3 imply a high level of homogeneity, as stated by de Pinho Neves et al, 2014 [37].…”

Section: Optimization and Verificationmentioning

confidence: 90%

“…Particle size is an essential element that affects the characteristics of nanoparticles [31]. The size of nanoparticles is affected by the stirring speed [19], [32].…”

Section: Nomentioning

confidence: 99%

“…The polydispersity index value indicates the uniformity of the particle size. A lower PDI value indicates the nanoparticle size distribution is relatively uniform [31]. PDI values below 0.3 indicate high homogeneity [37].…”

Section: Surface Response Analysis Of Polydispersity Index (Pdi)mentioning

confidence: 99%

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Optimization of Javanese Long Pepper (Piper retrofractum Vahl) extract nanoparticles as an aphrodisiac preparation

Rahman,

Sumarlan,

Sukardi

et al. 2024

E3S Web Conf.

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Piper retrofractum Vahl contains piperine compounds that, once extracted, have the disadvantages of low solubility and bioavailability. The enhanced solubility will significantly impact the absorption of medications in the body, resulting in rapid and noticeable results. Nanoparticle technology was developed to overcome this problem. The research aims to obtain optimum conditions for synthesizing Javanese long pepper extract nanoparticles under optimal particle size, Polydispersity Index (PDI), and piperine content. The research employed the Response Surface Methodology (RSM) method utilizing Central Composite Design (CCD) with a ratio factor of chitosan:STTP and the stirring speed. The utilization of RSM facilitated the optimization of nanoparticle manufacturing by considering various treatment parameters. RMS was selected due to its relevance as a tool for optimizing the manufacturing process of nanoparticles. The RMS result represents the optimal state of the nanoparticle manufacturing process, effectively addressing the study objectives. The research findings indicated that the most favorable circumstances for producing nanoparticles from Javanese long pepper extract were achieved with a chitosan:STTP ratio of 3,241:1 and a stirring speed of 826,133 rpm. The nanoparticles were obtained under these specific conditions, resulting in an optimal particle size of 33,884 nm, an optimal Polydispersity Index (PDI) of 0.675, and an optimal piperine content of 26,936%. This research provides valuable insights for the community and the pharmaceutical/herbal sector regarding using piperine compounds as therapeutic substances. Specifically, it highlights their potential to enhance bioavailability and absorption in the body.

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Section: Optimization and Verificationmentioning

confidence: 90%

“…Particle size is an essential element that affects the characteristics of nanoparticles [31]. The size of nanoparticles is affected by the stirring speed [19], [32].…”

Section: Nomentioning

confidence: 99%

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Optimization of Javanese Long Pepper (Piper retrofractum Vahl) extract nanoparticles as an aphrodisiac preparation

Rahman,

Sumarlan,

Sukardi

et al. 2024

E3S Web Conf.

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“…In addition, to delve into the development of a novel matrix for biomedical use, a recycling purpose is revealed, helping the environment at an ecological level since the residues from the processing of crustaceans are not used by the fishing companies of our country. Most studies in the scientific literature use analytical grade CS for particle design [44][45][46][47][48][49]. Moreover, we used Bet-rich BR Ext.…”

Section: Discussionmentioning

confidence: 99%

Chitosan-Based Nanogels Designed for Betanin-Rich Beetroot Extract Transport: Physicochemical and Biological Aspects

Silva Nieto,

Samaniego López,

Moretton

et al. 2023

Polymers

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Nanotechnology has emerged as a possible solution to improve phytochemicals’ limitations. The objective of the present study was to encapsulate beetroot extract (BR Ext) within a chitosan (CS)-based nanogel (NG) designed via ionic crosslinking with tripolyphosphate (TPP) for betanin (Bet) delivery, mainly in the ophthalmic environment. BR Ext is rich in betanin (Bet) according to thin layer chromatography (TLC), UV-visible spectroscopy, and HPLC analysis. NG presented a monodisperse profile with a size of 166 ± 6 nm and low polydispersity (0.30 ± 0.03). ζ potential (ζ-Pot) of +28 ± 1 is indicative of a colloidally stable system. BR Ext encapsulation efficiency (EE) was 45 ± 3%. TEM, with the respective 3D-surface plots and AFM, showed spherical–elliptical-shaped NG. The BR Ext release profile was biphasic with a burst release followed by slow and sustained phase over 12 h. Mucoadhesion assay demonstrated interactions between NG with mucin. Moreover, NG provided photoprotection and pH stability to BR Ext. FRAP and ABTS assays confirmed that BR Ext maintained antioxidant activity into NG. Furthermore, in vitro assays using human retinal cells displayed absence of cytotoxicity as well as an efficient protection against injury agents (LPS and H2O2). NGs are a promising platform for BR Ext encapsulation, exerting controlled release for ophthalmological use.

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“…Several subsequent studies have reported that this compound possesses strong antioxidant and antiinflammatory activities [22][23][24][25][26].Within this particular context, the primary objective of our study is to conduct an initial assessment of the impact of betanin consumption on the activity and gene expression of pivotal enzymes involved in gastric mucosal inflammation and ulceration.…”

Section: Introductionmentioning

confidence: 99%

The protective effects of betanin against experimental gastric ulcer by reduction of ROS and suppression of inflammatory genes via NF-κB, iNOS, COX-2 and TNF-α pathways

Alghamdi

2023

JCNB

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Betanin, a prominent compound found in beetroot, however, the potential protective effects of this compound against gastric damage and ulceration have yet to be explored. This study aims to investigate the impact of purified beetroot betanin (Bet) on the activities of inflammatory enzymes and gene expressions associated with inflammation in gastric tissues. Gastric ulcer rats were induced by ethanol at dose 5ml/kg body weight. Ulcerated rats were treated by purified betanin or omeprazole; from which some inflammation-related genes and gastric-inflammation enzymes were investigated using RT-PCR and biochemical analysis, respectively. Bet effectively suppressed the activities of key enzymes involved in gastric mucosal inflammation, including 5-Lipoxygenase (5-LO), Hyaluronidase (HAase), and myeloperoxidase (MPO), by 58%, 50%, and 45%, respectively. Docking studies showed Bet binding to catalytic sites or key regions for enzymes’ activities, thus explaining the inhibitory capacity. Bet ingestion decreased the expression levels of inflammation-related genes, factor-kappa B (NF-ϰB) by 57%. This down-regulation subsequently led to a reduction in the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and tumour necrosis factor-α (TNF-α). Bet ingestion supressed thiobarbituric acid reactive substance (TBARS) rates by 59%, augmented nitric oxide (NO) level by 107% and protected gastric tissues from damage and ulceration. Bet revealed gastro-protective effects as confirmed by a reduction in the ulceration surface area and the acidity by 75.9% and 40%, respectively, and a significant increase in the weight of mucus and the curative index by 117% and 74.5%, respectively. In conclusion, these outcomes of physiological and molecular-based investigations are indicative of potential use of Bet as a functional food additive for the prevention of gastric ulcer.

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Synthesis, structural characterization and in vitro pharmacological properties of betanin-encapsulated chitosan nanoparticles

Cited by 8 publications

References 34 publications

Optimization of Javanese Long Pepper (Piper retrofractum Vahl) extract nanoparticles as an aphrodisiac preparation

Optimization of Javanese Long Pepper (Piper retrofractum Vahl) extract nanoparticles as an aphrodisiac preparation

Chitosan-Based Nanogels Designed for Betanin-Rich Beetroot Extract Transport: Physicochemical and Biological Aspects

The protective effects of betanin against experimental gastric ulcer by reduction of ROS and suppression of inflammatory genes via NF-κB, iNOS, COX-2 and TNF-α pathways

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