Synthesis, structural characterization and DNA interaction studies on a mononuclear copper complex: Nuclease activity via self-activation

In vitro DNA and BSA-binding, cell imaging and anticancer activity against human carcinoma cell lines of mixed ligand copper(II) complexes

“…A high K SV value of 1.77 Â 10 5 M À1 for the complex (1) and relatively high K SV value of 0.38 Â 10 5 M À1 for the complex (2) was calculated, which illustrates the complex (1) has a high quenching efficiency and can compete for DNA-binding sites with EB [34]. This K SV is greater than or similar to the values obtained for other complexes with N-donar ligands: (Cu(II): K SV = 2.45 Â 10 4 M À1 ) [32], (Zn(II): K SV = 3.10 Â 10 4 M À1 ) [22] and (Ni(II): K SV = 8.8 Â 10 5 M À1 ) [23]. Moreover, to estimate the strength of the binding of the complex (1) with DNA the apparent binding constant (K app ) is calculated using the equation K EB [EB] = K app [Complex], where [EB] = 1.0 lM, K EB = 1.0 Â 10 7 M À1 [35], and the complex concentration was the value at 50% reduction of the fluorescence intensity of EB.…”

“…The K b value for complex (1) is 0.4 Â 10 4 M À1 , which is approximately similar to the K b value (0.2 Â 10 4 M À1 ) for complex (2). These observed K b for the interaction between the complex (1) and (2) with FS-DNA is very smaller than to the values obtained for the interaction between the complex (1) and (2) with CT-DNA [17] and is similar to other the Cu(II) complex with N-donar ligands: (Cu(II): K b = 5.2 Â 10 3 M À1 ) [32].…”

Synthesis, spectral, antitumor, antioxidant and antimicrobial studies on Cu(II), Ni(II) and Co(II) complexes of 4-[(1H-Benzoimidazol-2-ylimino)-methyl]-benzene-1,3-diol

“…The area of no growth around the disc is known as a "Zone of inhibition" or "Clear zone". For the disc diffusion, the zone diameters were measured with slipping calipers of the National Committee for Clinical Laboratory Standards [32,33]. Agar-based methods such as Etest and disk diffusion can be good alternatives because they are simpler and faster than brothbased methods [40,41].…”

“…Metal complexes of Schiff base compounds are a class of important compounds in medicinal and pharmaceutical field. They have wide applications in some biological aspects [26,[28][29][30][31][32][33] The present work aims to synthesize a new Schiff base ligand namely, 4-[(1H-Benzoimidazol-2-ylimino)-methyl]-benzene-1,3-diol and its complexes with Co(II), Ni(II) and Cu(II) ions. The complexes have been characterized by several tools of analyses such as elemental analyses, magnetic moment measurements, , ICP , molar conductance, ESR, IR, UV-Vis spectra.…”

Nucleolytic Property Promoted by Biologically Active Metal-Based Complexes Formed by Neutral Quatridentate Ligands: Synthesis, Characterization, and DNA Binding Studies

“…To achieve this, the electronic absorption spectra of the metal complexes of Schiff base ligands (L 1 and L 2 ) in the absence and presence of the CT DNA at different concentrations (at a constant concentration of the complexes) were measured (Table 2) and one representative [CuL 2 Cl 2 ] complex is given in Figure 5 [27,28] but much smaller than the classical intercalators and metallointercalators where binding constants reported in the order of 10 7 M −1 . [29] From the results, the [CuL 2 Cl 2 ] complex shows higher affinity towards CT-DNA.…”