Synthesis, Self‐Assembly, and Nucleic Acid Recognition of an Acylhydrazone‐Conjugated Cationic Tetraphenylethene Ligand

Abstract

Supramolecular polymers are of interest in the pursuit of multivalent nucleic acids recognition. However, their formation often relies on non‐covalent forces that are also at play in the interaction with nucleic acids. In this work, we designed a novel compound (TPE‐Gir) combining a tetraphenylethene aromatic core tethered to four quaternary ammoniums through acylhydrazone spacers, and we investigated in detail its self‐assembly and interaction with different types of nucleic acids. The spectroscopic analyses indicate the self‐assembly of regular fluorescent nanoparticles (observed by DLS and TEM) in the absence of nucleic acids, the strong propensity to intercalate into single‐stranded DNA, the ability to bind into the minor groove of double‐stranded DNA, and the selective binding to G‐quadruplex (G4) structures by fitting within a wide G4‐groove. Those recognition events are quantified by isothermal titration calorimetry and the proposed binding models are supported by docking simulations.

An Emission‐Based Probe for the Detection and Quantification of DNA and RNA

“…[23] In another work, they have designed a probe having the TPE core tethered to four quaternary ammonium groups that self-assemble into fluorescent nanoparticles and interact with several nucleic acids. [24] Zheng's group has employed a TPE-based macrocycle bearing diammonium tails to promote the electrostatic binding to the DNA chain. [25] Here we report anew dicationic water-soluble fluorescent compound as a reliable marker for the nucleic acids.…”

“…Similarly, Clément's group has developed a light‐up probe for G4‐binding with HT‐DNA using a water‐soluble tetracationic TPE [23] . In another work, they have designed a probe having the TPE core tethered to four quaternary ammonium groups that self‐assemble into fluorescent nanoparticles and interact with several nucleic acids [24] . Zheng's group has employed a TPE‐based macrocycle bearing diammonium tails to promote the electrostatic binding to the DNA chain [25]…”