Synthesis, self-assembly, and <i>in vitro</i> toxicity of fatty acids-modified <i>Bletilla striata</i> polysaccharide

Wang, Wenping; He, Shaolong; Hong, Tongtong; Zhang, Yumei; Zhang, Xia; Ma, Yan

doi:10.3109/21691401.2015.1129621

Cited by 24 publications

(15 citation statements)

References 29 publications

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“…The cell viability was tested by MTT assay at 490 nm using a model 550 microplate reader (Bio-Rad, Segrate, Italy). The percentage of cell viability was calculated using Equation (2).…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

“…These polymeric carbohydrates were composed of long chains of monosaccharide units joined together by glycosidic bonds. Generally, they are usually abundant in functional groups such as hydroxyl, amino and carboxylic acid groups, which can be partially substituted by grafting hydrophobic segments to obtain amphiphilic molecules [1,2]. The resulting amphiphilic polysaccharides can self-assemble to form core/shell-type micelles in aqueous solution at low critical micelle concentration (CMC).…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and evaluation of hydrophobically modified fenugreek gum for potential hepatic drug delivery

Zhou

Zhang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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The objective of this study was to hydrophobically modify fenugreek gum (FG) and to further evaluate the potential application of the obtained derivative in liver-targeted drug delivery system. Stearic acid (C 18 ) was conjugated with FG (FG-C 18 ) by a simple esterification reaction. The obtained FG-C 18 was then characterized on its chemical structure by Fourier transform infrared spectroscopy and 1 H-nuclear magnetic resonance. The self-assembled nanomicelles (NMs) of FG-C 18 in water were prepared by an ultrasonication method. The average diameter and zeta potential of FG-C 18 NMs were 196.70 ± 6.12 nm and À31.79 ± 1.58 mV, respectively. FG-C 18 NMs appeared as spherical particles under transmission electron microscopy and possessed a critical micellar concentration of 0.042 mg/ml by pyrene fluorescence probe method. A low toxicity of FG-C 18 was revealed on both HepG2 and MCF-7 cells at 0.1-100 mg/ ml. Haemolysis of FG-C 18 was less than 5%. Cellular uptake of coumarin-6 into HepG2 cells was enhanced by treating with C6-loaded FG-C 18 NMs compared to free coumarin-6. These results suggest that FG-C 18 have a potential application for a liver targeted drug delivery. ARTICLE HISTORY

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“…The cell viability was tested by MTT assay at 490 nm using a model 550 microplate reader (Bio-Rad, Segrate, Italy). The percentage of cell viability was calculated using Equation (2).…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis and evaluation of hydrophobically modified fenugreek gum for potential hepatic drug delivery

Zhou

Zhang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…According to our previous work, the average size of empty nanoparticles was 250 nm [16], which was smaller than that of the drug-loaded nanoparticles. The reason might be the encapsulation of a hydrophobic drug leads to a more compact core-shell structure.…”

Section: Resultsmentioning

confidence: 99%

“…SM-hm-BSP nanoparticles showed a significant inhibition effect on HepG2 cell proliferation compared to the SM solution. Since hm-BSP itself was proven to be a preferable cytocompatibility material [16], we assumed that the main reason for the difference in cytotoxicity was that the micelles had a high affinity for hepatocytes. To confirm this hypothesis, the cellular internalization capacity of HepG2 cells for free C6 and C6-hm-BSP nanoparticles were further investigated by fluorescence microscopy and fluorometry, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Bletilla striata polysaccharide (BSP), as an extract from the tubers of Bletilla striata , is a neutral water-soluble glucomannan with a backbone of (1 → 4)-linked β- d -mannose and glucose in a molar ratio of 3:1. In our previous study [16], fatty acids with different chain lengths were used to modify BSP, and the BSP hydrophobically modified by stearic acid (hm-BSP) showed a preferable self-assembly property, as well as, good biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

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Silymarin-Loaded Nanoparticles Based on Stearic Acid-Modified Bletilla striata Polysaccharide for Hepatic Targeting

et al. 2016

Molecules

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Silymarin has been widely used as a hepatoprotective drug in the treatment of various liver diseases, yet its effectiveness is affected by its poor water solubility and low bioavailability after oral administration, and there is a need for the development of intravenous products, especially for liver-targeting purposes. In this study, silymarin was encapsulated in self-assembled nanoparticles of Bletilla striata polysaccharide (BSP) conjugates modified with stearic acid and the physicochemical properties of the obtained nanoparticles were characterized. The silymarin-loaded micelles appeared as spherical particles with a mean diameter of 200 nm under TEM. The encapsulation of drug molecules was confirmed by DSC thermograms and XRD diffractograms, respectively. The nanoparticles exhibited a sustained-release profile for nearly 1 week with no obvious initial burst. Compared to drug solutions, the drug-loaded nanoparticles showed a lower viability and higher uptake intensity on HepG2 cell lines. After intravenous administration of nanoparticle formulation for 30 min to mice, the liver became the most significant organ enriched with the fluorescent probe. These results suggest that BSP derivative nanoparticles possess hepatic targeting capability and are promising nanocarriers for delivering silymarin to the liver.

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Screening of Bletilla striata, Bletilla ochracea and Oreorchis foliosa differential metabolites based on metabolomics

Niu

et al. 2022

Biomedical Chromatography

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As a representative medicinal plant in the Orchidaceae, Bletilla striata plays a variety of pharmacological roles in the clinic. However, the emergence of counterfeit species is affecting the basic medicinal materials source identification process, for which Bletilla ochracea and Oreorchis foliosa of the Orchidaceae are two representative species. For this study, 13 representative B. striata samples, three B. ochracea samples and three O. foliosa samples were selected for the systematic determination of polysaccharide yields and monosaccharide composition, and further detection of secondary metabolites by HPLC–MS. The results revealed that there was a significant difference in the yields of polysaccharides between B. striata and B. ochracea (p = 0.006). Although the polysaccharides of both species were composed of glucose and mannose, the molar ratio of the two monosaccharides was different, suggesting that the structures of the polysaccharides were different. The metabolomics results showed that there were no differences in the types of metabolites between B. striata and B. ochracea; however, there were differences in the contents of these metabolites. Although there was no significant difference in the polysaccharide yields of B. striata and O. foliosa (p = 0.074) and the monosaccharide composition was the same (glucose and mannose), many different metabolites were screened out between them: six compounds such as C36H34O11 existed only in B. striata, while substance C39H54O22 was unique to O. foliosa. Therefore, based on the analysis of the polysaccharide content and monosaccharide composition, combined with phase metabolomics research, a preliminary distinction between B. striata, B. ochracea and O. foliosa was achieved.

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Synthesis, self-assembly, and in vitro toxicity of fatty acids-modified Bletilla striata polysaccharide

Cited by 24 publications

References 29 publications

Synthesis and evaluation of hydrophobically modified fenugreek gum for potential hepatic drug delivery

Synthesis and evaluation of hydrophobically modified fenugreek gum for potential hepatic drug delivery

Silymarin-Loaded Nanoparticles Based on Stearic Acid-Modified Bletilla striata Polysaccharide for Hepatic Targeting

Screening of Bletilla striata, Bletilla ochracea and Oreorchis foliosa differential metabolites based on metabolomics

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