Synthesis, recyclization under the action of methanol and analgetic activity of N'-(5-aryl-2-oxofuran-3(2H)-ylidene)furan-2-carbohydrazides

Abstract

New methyl 5-aryl-1-(furan-2-carbonyl)-1H-pyrazole-3-carboxylates were obtained via decyclization reaction of N'-(5-aryl-2-oxofuran-3(2H)-ylidene)furan-2-carbohydrazides under the action of methanol. Starting N'-(5-aryl-2-oxofuran-3(2H)-ylidene)furan-2-carbohydrazides were obtained by intramolecular cyclization of substituted 4-aryl-2-[2-(furan-2-ylcarbonyl)hydrazinylidene]-4-oxobutanoic acids in propionic anhydride. The structure of the compounds obtained was confirmed by the 1H NMR spectroscopy, IR spectrometry and elemental analysis methods. Analgesic activity of some obtained compounds was studied by the “hot plate” method on outbred white mice of both sexes with intraperitoneal injection.

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“…Then, cells were incubated in the CO2 incubator at 37°C for 1 h. supernatants were removed from the wells, and then 100 µL DMSO was added to the reduced MTT dye. The final absorbency measurements were determined using a plate read at 440 nm and the values [12][13][14][15] .…”

“…Further, the crude was purified by column chromatogram using dichloromethane and ethyl acetate (3:1) as eluants Fig. 3 [11][12][13][14][15][16] . The purified product w as dried under vacuum and recrystallized fro m ethanol at rt to furnish the product.…”