Synthesis, Platelet Adhesion and Cytotoxicity Studies of New Glycerophosphoryl-Containing Polyurethanes

Abstract

In this work we synthesized new MDI–-based poly(ether)urethanes (PEUs) with phospholipid-like residue as chain extender. Polymers were prepared by a conventional two-step solution polymerization procedure using 4,4'-diphenylmethanediisocyanate (MDI) and poly(1, 4-butanediol) with 1000 as molecular weight to form prepolymers which were successively polymerized with 1-glycerophosphorylcholine (1-GPC), 2-glycerophosphorylcholine (2-GPC) or glycerophosphorylserine (GPS) as chain extenders. Two reference polymers bearing 1,4-butandiol (BD) have been also synthesized. The polymers obtained were characterized by Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC) and modulated scanning calorimetry (MDSC). The biocompatibility of synthesized segmented polyurethanes was then investigated by platelet-rich plasma contact studies and related scanning electron microscopy (SEM) photographs for blood compatibility and cytotoxicity assay (MTT test) on material elution to assess the effect of any toxic leachables on cellular viability. Three polymers among all have given very satisfactory results suggesting to investigate more deeply their possible use in biomedical devices.

Plasma functionalization of polycarbonaturethane to improve endothelialization—Effect of shear stress as a critical factor for biocompatibility control

“…[1][2][3][4] However, thrombus formation remained a critical risk factor. [5][6][7] An effective way to improve hemocompatibility of PCU is to optimize its material surface but not significantly change their intrinsic mechanical properties. Modification of the polyurethane surface chemistry combining reduced platelet adhesion/activation and increasing endothelial attachment would be an attractive task.…”

Hydrophilic PCU scaffolds prepared by grafting PEGMA and immobilizing gelatin to enhance cell adhesion and proliferation

“…The high hydrophobicity of PCU artificial blood scaffolds is one of the main reasons that account for these problems [10]. To overcome this obstacle, many strategies have been investigated by researchers.…”

Functionalization of Polycarbonate Surfaces by Grafting PEG and Zwitterionic Polymers with a Multicomb Structure

“…Polycarbonateurethanes (PCUs) have been widely used as materials for medical applications in recent years as they provide compared to other synthetic materials relatively beneficial hemocompatibility and mechanical properties . However, thrombus formation could still occur when artificial organs and biomedical devices made of PCU were in contact with blood for extended time periods . In fact, even a small amount of proteins on a surface of implant material could lead to unwanted adhesion.…”