Synthesis, physicochemical characterization and biological activity of synthesized Silver and Rajat Bhasma nanoparticles using Clerodendrum inerme

Abstract

Bhasmais Metallo-medicine and made from metals and minerals. Rajatbhasma or Silver Bhasma belongs to a group of nanoparticles that have medicinal values and are used in Ayurveda as Drugs against various ailments. Clerodendrum inermea traditionally well-accepted plant is used extensivelyin ayurvedic therapeutic formulations, but till date no major steps have been carried out to validate the scientific relevance of synthesized nanoparticles from Rajatbhasma using C. inerme. Therefore, in the present study biosynthesized nanoparticles were characterized by UV–Vis spectroscopy, SEM, FESEM and EDX analysis whereas, a comparative study has also been made to check the antioxidant and antimicrobial activity of synthesized silver and rajatbhasma nanoparticle. The SEM and FESEM analysis revealed that the synthesized nanoparticles are well shaped and the average particle size ranges between 30–90 nm and 10-50 nm respectively. In the case of EDX analysis, the highest peak at ~3Kev in the case of synthesized silver and rajatbhasma nanoparticle supports the formation of silver nanoparticles. Subsequently, antioxidant and antimicrobial activities of the synthesized nanoparticles showed excellent results when compared to the standard. The obtained results may provide support in the field of therapeutics and drug delivery and might prove beneficial as a novel drug candidate against bacterial infection in the future.

An Appraisal on Antimicrobial Activity of Herbomineral Formulations

“…Swaskuthara Rasa was found to effective against the Klebsiella pneumonia (32). Rajat Bhasma shown significant results on Gram positive bacteria (33). Herbal drugs used in these herbomineral formulations itself have significant antimicrobial property like-Vatsnanabha (Aconitum ferox Wall ex Seringe) (34), Sunthi (Zingiber officinale Linn.)…”

“…29 mm and other strains were registrant to Rajat Bhasma and its silver naoparticle. The study was compared with Streptomycin Sulphate as standard antimicrobial agent (13).…”

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“…[14,15] Field emission scanning electron microscopy with EDX (FESEM-EDS) was also used in quantitative analysis of Swarna Makshika Bhasma and Rajat Bhasma. [16,17] SEM-EDX analysis along with pharmacodynamics study of any mineral compound can provide the accurate estimation of bioavailability as well as safety dose estimation of study compound. [18,19] SEM-EDX quantitative and qualitative analysis is also useful to maintain the inherent quality of the ancient Indian system of medicine.…”