Synthesis, optoelectronic and thermal characterization of PMMA-MWCNTs nanocomposite thin films incorporated by ZrO2 NPs

Migdadi, A. B.; Alsaad, Ahmad; Telfah, Ahmad

doi:10.1007/s10854-022-07699-8

Cited by 13 publications

(1 citation statement)

References 63 publications

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“…ZrO 2 -NP are also used in drug delivery systems for itraconazole and bisphosphonates (Keleştemur and Yeşilay, 2020; Talegaonkar et al, 2022). Additionally, they have been used in optical coating, and also as a catalyzing agent in organic reactions (Azmy et al, 2022; Migdadi et al, 2022; Salem et al, 2022; Ullah et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Zirconium dioxide nanoparticles induced cytotoxicity in rat cerebral cortical neurons and apoptosis in neuron-like N2a and PC12 cell lines

Alavi,

Asadpour,

Boroushaki

et al. 2024

Toxicol Ind Health

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During recent decades, the application of zirconium dioxide nanoparticles (ZrO2-NP) has been expanded in various fields ranging from medicine to industry. It has been shown that ZrO2-NP has the potential to cross the blood–brain barrier (BBB) and induce neurotoxicity. In the current study, we investigated the in vivo neurotoxicity, as well as, the cellular mechanism of ZrO2-NP toxicity on two neuronal-like cell lines, PC12 and N2a. PC12 and N2a cells were exposed to increasing concentrations of ZrO2-NP (0-2000 µg/ml) for 48 h. The apoptotic effect of ZrO2-NP was determined using annexin V/propidium iodide double staining (by flow cytometry), and western blot analysis of relative apoptotic proteins, including caspase-3, caspase-9, bax, and bcl2. Based on our results, ZrO2-NP at concentrations of 250-2000 μg/mL increased both early and late-stage apoptosis in a concentration-dependent manner. Moreover, the expressions of cleaved-caspase-3 and -9 proteins and the bax/bcl2 ratio were significantly increased. In addition, oral administration of ZrO2-NP (50 mg/kg) to male Wistar rats for 28 days led to the loss of neuronal cells in the cerebral cortex. Taken together, our findings highlighted the role of apoptosis on cytotoxicity induced by ZrO2-NP.

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