Synthesis of zinc oxide nanoparticles and its conjugation with antibiotic: Antibacterial and morphological characterization

Tyagi, Pankaj; Gola, Deepak; Tyagi, Shruti; Mishra, Ankit; Kumar, Ajay; Chauhan, Nitin; Ahuja, Anami; Sirohi, Sandeep

doi:10.1016/j.enmm.2020.100391

Cited by 18 publications

(10 citation statements)

References 29 publications

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“…In regard to antibacterial activity, synergistic effects were observed when ZnONPs were used in conjugation with antibiotics against all tested bacterial strains. 150 5.1.4. Method D. In the last approach, the synergistic effect was achieved by synthesizing MNPs using antibiotics as reducing agents, converging two steps in one (method D, Table 5).…”

Section: Therapeutic Agents Conjugated With Mnps and Antimicrobial Ef...mentioning

confidence: 99%

“…The amine functionalization was obtained by a chemical process using 3-ethyldimethylaminopropyl carbodiimide (EDC) and N -hydroxysuccinimide (NHS). In regard to antibacterial activity, synergistic effects were observed when ZnONPs were used in conjugation with antibiotics against all tested bacterial strains …”

Section: Therapeutic Agents Conjugated With Mnps and Antimicrobial Ef...mentioning

confidence: 99%

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Synergistic Effects Between Metal Nanoparticles and Commercial Antimicrobial Agents: A Review

Ribeiro

Dias

Zille

2022

ACS Appl. Nano Mater.

111

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Nanotechnology has expanded into a broad range of clinical applications. In particular, metal nanoparticles (MNPs) display unique antimicrobial properties, a fundamental function of novel medical devices. Combining MNPs with commercial antimicrobial drugs (e. g., antibiotics, antifungals and antivirals) may offer several opportunities to overcome some disadvantages of their individual use and enhance effectiveness. MNPs-conjugates display multiple advantages. As drug delivery systems, the conjugates can extend the circulation of the drugs in the body, facilitate intercellular targeting, improve drug stabilization, and possess superior delivery. Concomitantly, they reduce the required drug dose, minimize toxicity and broaden the antimicrobial spectrum. In this work, the common strategies to combine MNPs with clinically used antimicrobial agents are underscored. Furthermore, a comprehensive survey about synergistic antimicrobial effects, mechanism of action and cytotoxicity is depicted. SilverAntimicrobial, drug deliver, anticancer, anti-angiogenic and biosensors 46,47 Gold Drug delivery, catalyst for medical therapy, antimicrobial conjugations, anticancer, gene therapy and diagnostic 48,49 Copper and copper oxide Antimicrobial and catalysis 50, 51 Iron and iron oxideAnticancer therapy, magnetic resonance imaging, targeted drug delivery and cell separation catalysis [52][53][54] Zinc oxide Personal care products, coatings, drug delivery, anticancer and antimicrobial 55, 56Aluminum oxide Drug delivery, biosensing, cancer therapy, antimicrobial, biomolecular preservation, immunotherapy 57 Titanium oxideDrug delivery, photodynamic therapy, cell imaging, biosensors, and genetic engineering, antimicrobial 58,59 Platinum Biomedical devices, anticancer therapies, cardiovascular diseases, bioimaging, nanozymes, biosensors, antimicrobial 60, 61 PalladiumPhotothermal agents, photoacoustic agents, antimicrobial, anticancer, gene/drug carriers, prodrug activators and biosensors 62,63 Scheme 1. MNPs used in biomedical applications. Silver Nanoparticles (AgNPs)AgNPs are the most prevalent inorganic nanoparticles applied as antimicrobial agent. AgNPs have demonstrated high antimicrobial activity comparable to Ag ionic form. However, several concerns have emerged regarding their cytotoxicity. The toxicity mechanisms, long-term accumulation effects and dose-response relationship are still grievously unknown. 64 Gold Nanoparticles (AuNPs)AuNPs are extremely valuable in developing antibacterial agents due to their low toxicity, high propensity for functionalization, eclectic effects, easy detection and photothermal activity. AuNPs per se possess very low antimicrobial activity, but numerous studies on the antimicrobial activity of AuNPs conjugated with small molecules, such as drugs, vaccines and antibodies, have been reported.

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Section: Therapeutic Agents Conjugated With Mnps and Antimicrobial Ef...mentioning

confidence: 99%

Section: Therapeutic Agents Conjugated With Mnps and Antimicrobial Ef...mentioning

confidence: 99%

Synergistic Effects Between Metal Nanoparticles and Commercial Antimicrobial Agents: A Review

Ribeiro

Dias

Zille

2022

ACS Appl. Nano Mater.

111

View full text Add to dashboard Cite

show abstract

“…This conjugate increased the antibacterial activity against Klebsiella spp. and E. coli by 4.3- and 2.7-fold, respectively, compared to ZnO-NPs alone [ 139 ]. Moreover, a recent study investigated the effect of ZnO NPs in combination with meropenem, ciprofloxacin, and colistin against P. aeruginosa ATCC strain and clinical isolate strains of P. aeruginosa .…”

Section: New Therapeutic Strategies: Combinatorial Treatmentsmentioning

confidence: 99%

Nanomaterials-Based Combinatorial Therapy as a Strategy to Combat Antibiotic Resistance

et al. 2022

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Since the discovery of antibiotics, humanity has been able to cope with the battle against bacterial infections. However, the inappropriate use of antibiotics, the lack of innovation in therapeutic agents, and other factors have allowed the emergence of new bacterial strains resistant to multiple antibiotic treatments, causing a crisis in the health sector. Furthermore, the World Health Organization has listed a series of pathogens (ESKAPE group) that have acquired new and varied resistance to different antibiotics families. Therefore, the scientific community has prioritized designing and developing novel treatments to combat these ESKAPE pathogens and other emergent multidrug-resistant bacteria. One of the solutions is the use of combinatorial therapies. Combinatorial therapies seek to enhance the effects of individual treatments at lower doses, bringing the advantage of being, in most cases, much less harmful to patients. Among the new developments in combinatorial therapies, nanomaterials have gained significant interest. Some of the most promising nanotherapeutics include polymers, inorganic nanoparticles, and antimicrobial peptides due to their bactericidal and nanocarrier properties. Therefore, this review focuses on discussing the state-of-the-art of the most significant advances and concludes with a perspective on the future developments of nanotherapeutic combinatorial treatments that target bacterial infections.

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“…Antibacterial Potential. The antibacterial potential of biogenic FeNPs derived from BPE and SLE was tested using the agar well diffusion method against two bacterial strains: Bacillus subtilis (MTTC 1133) and Escherichia coli (MTTC 62) obtained from the Microbial Type Culture Collection and Gene Bank (MTCC), Chandigarh, India, and the zone of inhibition was measured in millimeters using the agar well diffusion method described by Thiyagarajan et al [7,[30][31][32]. Antibiotics such as chloramphenicol and gentamicin were used as positive controls in the study.…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

Green Synthesis of Iron Nanoparticles from Spinach Leaf and Banana Peel Aqueous Extracts and Evaluation of Antibacterial Potential

Tyagi¹,

Gupta²,

Tyagi

et al. 2021

Journal of Nanomaterials

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Spinacia oleracea (spinach) and Musa acuminata (banana) were chosen for the study, and aqueous extracts of spinach leaf extract (SLE) and banana peel extract (BPE) were prepared for the synthesis of iron nanoparticles (FeNPs), and their antibacterial potential against pathogenic bacteria Bacillus subtilis (MTTC 1133) and Escherichia coli (MTTC 62) was evaluated. In 10 minutes at 60°C, the color of the mixture (FeCl3+SLE) changed from light green to dark blackish-brown, and the color of the mix (FeCl3+BPE) changed from transparent yellow to dark black, confirming the synthesis of FeNPs from SLE and BPE, respectively. The UV-Vis spectra of spinach- and banana-derived FeNPs revealed two peaks ranging from 240 to 430 nm and multiple peaks at 240, 270, and 395 nm, respectively. FTIR spectroscopy was used to show different functional groups on BPE and SLE, and their role in FeNP synthesis was predicted. TEM micrographs showed that the particles were in nanoscale, ranging in size from 20 to 50 nm for BPE-derived FeNPs and 10 to 70 nm for SLE-derived FeNPs. The FeNP (BPE and SLE) XRD analysis revealed amorphism, with a weak iron characteristic peak, indicating noncrystallinity. The antibacterial potential of BPE- and SLE-FeNPs was investigated, and inhibition zones (mm) against B. subtilis ( 22.70 ± 0.4 ) and E. coli ( 20.45 ± 1.66 ) were observed, as well as SLE-FeNPs against B. subtilis ( 23.56 ± 1.00 ) and E. coli ( 20.33 ± 0.58 ). There were no significant differences in antibacterial activities between BPE-FeNPs and SLE-FeNPs. Positive controls were tetracycline and gentamicin, both standard antibiotics, at 5 μg/disk. SLE- and BPE-derived green FeNPs were also analysed in vivo of D. melanogaster life history traits, i.e., fecundity, hatchability, viability, and duration of development for toxicity evaluation. SLE- and BPE-derived green FeNPs at a concentration of 10 mg/L were fed flies compared to normal diet-fed flies (control sample), and no significant differences were observed between them. The findings suggest that FeNPs have a high antibacterial potential and could be used as antibacterial agents against pathogenic bacteria while being nontoxic in nature.

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Synthesis of zinc oxide nanoparticles and its conjugation with antibiotic: Antibacterial and morphological characterization

Cited by 18 publications

References 29 publications

Synergistic Effects Between Metal Nanoparticles and Commercial Antimicrobial Agents: A Review

Synergistic Effects Between Metal Nanoparticles and Commercial Antimicrobial Agents: A Review

Nanomaterials-Based Combinatorial Therapy as a Strategy to Combat Antibiotic Resistance

Green Synthesis of Iron Nanoparticles from Spinach Leaf and Banana Peel Aqueous Extracts and Evaluation of Antibacterial Potential

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