large pore volume and high surface area. [1-3] ZIF-8, pioneered by the group of Yaghi in 2006, [4] is synthesized from commercially available sources: 2-methyl imidazole (Hmim), serving as a linker, and zinc salts, giving Zn 2+ cations as nodes. Various synthetic methods for the formation of ZIF-8 have been reported. Those include different strategies toward powder ZIF-8, [1-18] as well as layer-by-layer (lbl) approaches leading to ZIF-8 deposited on supports. [19-23] The latter allow the synthesis of monolithic surface-anchored thin films of ZIF-8 (ZIF-8 SURMOF). Such surface-anchored MOFs (SURMOFs) show a high degree of crystallite orientation, controllable thickness, low defect densities, and therefore carry a high potential for thin-film applications. [22,23] In the recent past it has been recognized that, due to their permanent porosity, MOFs are perfect hosts for the incorporation of different guest species inside their pores, [24-26] including metal nanoparticles (mNPs). [27-31] For example, Esken et al. have prepared a Au/ ZIF-8 composite by the ship-in-the-bottle method, where volatile [Au(CO)Cl] was immobilized into ZIF-8 by gas-phase infiltration, and then reduced by CO to form Au NPs/ZIF-8. [32] Very recently, ZIF-8 with imbedded Ag/AgCl nanoparticles The first example of layer-by-layer growth of a metal-organic framework (MOF) directly on transmission electron microscopy (TEM) grids is described. ZIF-8 is deposited on thin amorphous carbon films and subjected to a structure analysis by (scanning) TEM ((S)TEM). This method serves as a two-in-one synthesis and TEM sample-preparation technique and allows straightforward analysis of ZIF-8 crystallites. Artifacts resulting from sample preparation are completely avoided by this approach. The morphological properties, crystal structure, and the chemical composition of the material are investigated with high spatial resolution by a variety of methods of (analytical) electron microscopy. Furthermore, the incorporation of metallic nanoparticles in ZIF-8 by integrating a corresponding step into the layer-by-layer deposition process is examined. The formation of ZIF-8 crystals on the film proceeds as under the absence of nanoparticle-forming synthesis steps. However, the nanoparticles rather cover the supporting amorphous carbon film than being incorporated in the ZIF-8 material. This information cannot be obtained from standard characterization techniques but requires the application of analytical (S)TEM techniques.