Synthesis of tubulin‐enriched fraction in rat visual cortex is modulated by dark‐rearing and light‐exposure

Rose, Steven; Sinha, Arun K.; Jones-Lecointe, Altheia

doi:10.1016/0014-5793(76)80464-4

Cited by 26 publications

(5 citation statements)

References 6 publications

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“…It is interesting that during maturation of rat brain there were postulated to be changes in the phosphorylation pattern of MAP 2 (Binder et al, 1984) as well as changes in the ability of MAP 2 to cause an assembly of tubulin (Francon et al, 1982). However, the correlation between the postulated increase during development in MAP 2 phosphorylation (Binder et al, 1984) (Rose et al, 1976) the rate of tubulin turnover is lower in dark-reared than in normally reared rats but increases 2fold within the first hour after light exposure. It may be that these changes also occur in cat visual cortices as well, concomitant with the change in the state of phosphorylation of MAP 2.…”

Section: Discussionmentioning

confidence: 84%

Ontogenetic changes in the cyclic adenosine 3',5'-monophosphate- stimulatable phosphorylation of cat visual cortex proteins, particularly of microtubule-associated protein 2 (MAP 2): effects of normal and dark rearing and of the exposure to light

Aoki¹,

Siekevitz²

1985

J. Neurosci.

130

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Based on a theory that a norepinephrine-stimulated cascade of events resulting in an increase of intracellular cyclic adenosine 3',5'-monophosphate (cAMP) modulates the state of plasticity for the receptive field property of visual cortical neurons, we have followed the ontogenetic changes in cAMP-stimulated phosphorylation of proteins in whole homogenates obtained from developing visual cortices of cats. In vitro phosphorylation was assayed with and without cAMP and the cAMP-dependent protein kinase, and the phosphoproteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were counted for 32P incorporated from [gamma-32P]ATP. It was found that the regulatory subunits of the cAMP-dependent protein kinase are present and fully active by birth, whereas the synapsin content increases at a rate concomitant with synaptogenesis. These ontogenetic developments are not influenced by dark rearing (DR) from birth, a procedure which postpones the onset of the critical period (CP) for plasticity. By contrast, the cAMP-stimulatable phosphorylation of microtubule-associated protein 2 (MAP 2), which under normal rearing conditions increases from birth to the second month, is strongly modulated by the presence of light in the environment. After DR for various periods, kittens were subsequently exposed to light so as to trigger the onset of the CP that had been postponed. A few hours of light were sufficient to cause a large increase in the in vitro phosphorylation of MAP 2. This effect is not observed in the auditory cortex or the lateral geniculate nucleus of the same animals, or in the visual cortex of normally reared cats which were then dark reared in adulthood. But this effect was seen in the visual cortices of cats following 5 months of DR from birth, animals which by chronological age have passed the CP, presumably because the onset of the CP was extended by the DR procedure. The cAMP-dependent phosphorylation of MAP 2 (and its dephosphorylation) may be an important factor for determining the state of plasticity in the CP through its affecting the dendritic cytoskeletal organization involving tubulin and actin.

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Section: Discussionmentioning

confidence: 84%

Ontogenetic changes in the cyclic adenosine 3',5'-monophosphate- stimulatable phosphorylation of cat visual cortex proteins, particularly of microtubule-associated protein 2 (MAP 2): effects of normal and dark rearing and of the exposure to light

Aoki¹,

Siekevitz²

1985

J. Neurosci.

130

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“…We have recently arrived at a comparable conclusion concerning modifiable tubulin of the chick brain consequent upon learning (Mileusnic et al, 1979). In discussing the earlier results (Stewart and Rose, 1978), two explanations were offered for the observation that, whilst light exposure results in a lasting increase in the rate of incorporation of precursor ["Hllysine into a tubulin-enriched fraction (Rose et al, 1976), the increase in total tubulin quantity as assessed by colchicine binding was transient. Either the excess tubulin was sequestered in a colchicine-inaccessible form or the onset of light exposure triggered an increase in the rates both of synthesis and of degradation of tubulin, with the mobilization of the degradative system lagging behind that of the synthetic, resulting in a transient overproduction.…”

Section: Resultsmentioning

confidence: 99%

“…Several biochemical measures are changed after first exposure of 50-day-old dark-reared animals t o light (Rose, 1977). It has previously been shown that following a I-h exposure of dark-reared rats to light, the incorporation of [3H]lysine into a repolymerized tubulin-enriched fraction from the visual cortex is increased (Rose et al, 1976). Futhermore, a transient elevation in the binding of intraperitoneally administered colchicine t o acidinsoluble material in the visual cortex was observed after 3-h exposure to light (Stewart and Rose, 1978).…”

Section: First Exposure Of Dark-reared Rats Colchicine Binding Capacimentioning

confidence: 98%

First Exposure of Dark‐reared Rats Colchicine Binding Capacity of to Light Increases Visual Cortex

Potempska

Rose

1980

Journal of Neurochemistry

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“…MAP2 phosphorylation changes the microtubule binding activity and the stability of the microtubule network (Brugg & Matus, 1991). Dark rearing also reduced the tubulin production rate, whereas light stimulation increased it (Rose et al ., 1976). The amount of MAP2 was also decreased in dark rearing, and ten days' light stimulation recovered it to the normal level (Reid & Daw, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…It still remains to be elucidated how NF‐L phosphorylation is involved in or interacts with molecular mechanisms previously proposed to operate in the cortical plasticity (Kasamatsu & Pettigrew, 1976; Hensch et al ., 1998; Huang et al ., 1999; Katz, 1999; Nedivi, 1999). Previous studies showed that visual experience after dark rearing induced alteration of the phosphorylation state of microtubule‐associated protein‐2 (MAP2) prepared from cat visual cortex (Aoki & Siekevitz, 1985, 1988; Rose et al ., 1976; Reid & Daw, 1995). In the visual cortex of dark‐reared cats, MAP2 is fully phosphorylated, and it is dephosphorylated immediately after exposure to light (Aoki & Siekevitz, 1985, 1988).…”

Section: Discussionmentioning

confidence: 99%

Visual stimulation‐induced phosphorylation of neurofilament‐L in the visual cortex of dark‐reared rats

Hashimoto

Nakamura

Imamura³

et al. 2001

Eur J of Neuroscience

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In dark-reared animals, visual exposure is expected to induce drastic changes in both the physiology and anatomy of the cortical neurons, including the rearrangement of their cytoskeletal structures. Phosphorylation of neurofilament-L (NF-L) is probably associated with relatively short-term structural plasticity in vivo, because the assembly and disassembly of the filaments are regulated by phosphorylation of the head domain of NF-L. Thus, by using a series of site- and phosphorylation state-specific antibodies against NF-L, we examined how visual activation induces the phosphorylation of NF-L in the rat brain. We found no specific immunoreactivity for phosphorylated NF-L in the brain of naive rats, whereas one-hour ambient light exposure after dark rearing for ten weeks from birth induced marked phosphorylation of NF-L selectively. Also, the NF-L phosphorylation was found to be localized in the primary and secondary visual cortical areas. These findings suggest that the selective phosphorylation of NF-L plays an important role in the structural plasticity related to the visual experience.

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Synthesis of tubulin‐enriched fraction in rat visual cortex is modulated by dark‐rearing and light‐exposure

Cited by 26 publications

References 6 publications

Ontogenetic changes in the cyclic adenosine 3',5'-monophosphate- stimulatable phosphorylation of cat visual cortex proteins, particularly of microtubule-associated protein 2 (MAP 2): effects of normal and dark rearing and of the exposure to light

Ontogenetic changes in the cyclic adenosine 3',5'-monophosphate- stimulatable phosphorylation of cat visual cortex proteins, particularly of microtubule-associated protein 2 (MAP 2): effects of normal and dark rearing and of the exposure to light

First Exposure of Dark‐reared Rats Colchicine Binding Capacity of to Light Increases Visual Cortex

Visual stimulation‐induced phosphorylation of neurofilament‐L in the visual cortex of dark‐reared rats

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