Synthesis of the matriglycan hexasaccharide, -3Xylα1-3GlcAβ1-trimer and its interaction with laminin

Tamura, Takahiro; Omura, Yuka; Kotera, Kota; Ito, Ryota; Ohno, Shiho; Manabe, Noriyoshi; Yamaguchi, Yoshiki; Tamura, Jun’ichi

doi:10.1039/d2ob01388f

Cited by 1 publication

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References 20 publications

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“…These techniques allow either highly sensitive quantitative detection or in situ imaging of glycans on cells, which are summarized in Table 3. In addition, biomolecular layer interferometry (BLI) [102,103], microscale thermophoresis (MST) [104,105], lectin histochemistry [106][107][108][109], and enzyme-linked lectin assay (ELLA) [110,111] are also powerful tools for studying glycan recognition. However, these techniques are rarely applied to in situ sensing at the cell surface and are therefore not included in this review.…”

Section: Glycan-specific Sensingmentioning

confidence: 99%

Cell-Surface Glycan Labeling and Sensing

Li,

Wang,

Ding

et al. 2023

Targets

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Cell-surface glycans are abundant and complex and play a critical role in maintaining protein stability, regulating cell behavior, and participating in cell communication. Obtaining structural information on glycans in situ is helpful to further understand the role of glycans in the physiological and pathological processes of cells and the regulatory mechanism. To achieve this, we can use recognition or labeling strategies to convert the presence of glycans on the cell surface into signals that can be detected. Currently, many different types of in situ sensing strategies for glycans have been developed. The spatial control of the conversion process can realize the restriction of glycan detection to specific proteins, and the introduction of signal amplification technology into the conversion process can improve the sensitivity of sensing. In this paper, the recent progress of glycan labeling methods and sensing technology is reviewed, and the future development direction is prospected.

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