Synthesis of the major adult cuticle proteins of Drosophila melanogaster during hypoderm differentiation

Roter, Alan; Spofford, Janice B.; Swift, Hewson

doi:10.1016/0012-1606(85)90324-0

Cited by 29 publications

(10 citation statements)

References 18 publications

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“…The delayed appearance of LCPs 1 and 2 correlates with delayed accumulation of the mRNAs encoding these two proteins, suggesting that the level of control responsible for the differential deposition of these LCPs is at least pretranslational, if not transcriptional. Roter [12] has also demonstrated time-dependent changes in the accumulation of ACPs. The implication from these results is that differential distribution of CPs in procuticles-from outside to inside-will be found in third instar larval and adult cuticles in addition to pupal cuticles.…”

Section: Pupal Cuticle Formation In Vivomentioning

confidence: 92%

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Ecdysone regulation of cuticle protein gene expression inDrosophila

Fristrom

Alexander

Brown

et al. 1986

Arch. Insect Biochem. Physiol.

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The deposition and apolysis of insect cuticles have long been known to be regulated by ecdysone. Unsclerotized, chitin-containing procuticles contain evolutionarily conserved, hydrophobic proteins that are soluble in solutions of denaturing agents. The pupal procuticle of Drosophila is deposited by larval and imaginal epidermis starting 9 h after puparium formation when the ecdysone titer is low. Initially, a set of low molecular weight proteins (less than 25,000 daltons; low molecular weight pupal cuticle proteins = S-PCPs) is synthesized. However, about the time of pupation, synthesis of S-PCPs ceases, and high molecular weight proteins (greater than 50,000 daltons; HPCPs) are synthesized. In vitro experiments indicate that the initial formation of the procuticle with synthesis of the S-PCPs requires a pulse of hormone followed by withdrawal (6 h with 20-hydroxyecdysone, 1 pg/ml). The switch from synthesis of S-PCPs to H-PCPs is facilitated by a second, short pulse of 20-hydroxyecdysone (0.1 pglml, 3 h). Ultrastructural localization demonstrates that the S-PCPs are located only in the external lamellae of the procuticle, while the H-PCPs are present only in internal lamellae. Developmental analyses with cloned genes indicate that cuticle protein genes are expressed during only one stage of Drosophila development. Some of the genes encoding S-PCPs are limited in their expression to larval (posterior) or imaginal (anterior) epidermis. Preliminary molecular analyses of the larval and pupal cuticle protein genes indicate that they are organized in different ways. For example, four larval genes exist in a cluster with divergent transcription, and one PCP gene, PCP-CART, is located within an intron of a "housekeeping" gene.

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Section: Pupal Cuticle Formation In Vivomentioning

confidence: 92%

“…Low-molecular-weight proteins are also present in extracts from pupal and larval cuticles. In additions, these extracts contain high-molecular-weight and basic proteins [7,12].…”

Section: Introductionmentioning

confidence: 99%

Ecdysone regulation of cuticle protein gene expression inDrosophila

Fristrom

Alexander

Brown

et al. 1986

Arch. Insect Biochem. Physiol.

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“…Its chemical nature remains unclear, but known aspects appear conserved among insects, for example between Drosophila and Rhodnius prolixus (see Locke, 2001 for review). Cuticle comprises hundred of proteins (Roter et al, 1985;Andersen et al, 1995), chitin (a polysaccharide composed essentially of N-acetylglucosamine) and lipids. Modification of the protein matrix by quinone compounds (sclerotization) determines cuticle pigmentation (Riddiford and Hiruma, 1988) and rigidity, while local protein composition (Gosline et al, 2002) also influences its mechanical properties (e.g.…”

Section: Basic Structure Of the Larval Cuticlementioning

confidence: 99%

Genetic control of epidermis differentiation in Drosophila.

Payre¹

2004

Int. J. Dev. Biol.

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In arthropods, the animal body is isolated from the external environment by a protective exoskeleton called the cuticle. The cuticle of young larvae has certainly been the most scrutinized structure in Drosophila and genetic studies of the pattern of cuticular extensions has provided the main source of our comprehension of the control of embryonic development. However, the complex structure of the cuticle remains poorly understood and analysis of the underlying epidermis has started only recently. Here I review different aspects of epidermis differentiation with the aim of presenting an integrated view of the organisation of the Drosophila integument. Although profound differences in epidermis organisation are observed across species, accumulated results suggest that epidermis formation and differentiation might share an unsuspected number of homologies between Drosophila and vertebrates.

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“…The RNA was loaded in Hybond-N ϩ membranes (Amersham Biosciences, Piscataway, NJ) by using a slot blot manifold (Hoeffer, San Francisco, CA). The blot was hybridized with labeled Pcp-1 and Actin probes (Roter et al, 1985;Vigoreux and Tobin, 1987) in a 50% formamide hybridization solution. After hybridization, the membrane was washed at 65°C several times in 0.2ϫ SSC, 0.1% SDS and exposed to XAR-5 film (Eastman Kodak, Rochester, NY).…”

Section: Slot Blot Hybridizationmentioning

confidence: 99%

DNA Repair and Transcriptional Effects of Mutations in TFIIH inDrosophilaDevelopment

Merino

Reynaud

Vázquez

et al. 2002

MBoC

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Mutations in XPB and XPD TFIIH helicases have been related with three hereditary human disorders: xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy. The dual role of TFIIH in DNA repair and transcription makes it difficult to discern which of the mutant TFIIH phenotypes is due to defects in any of these different processes. We used haywire (hay), the Drosophila XPB homolog, to dissect this problem. Our results show that when hay dosage is affected, the fly shows defects in structures that require high levels of transcription. We found a genetic interaction between hay and cdk7, and we propose that some of these phenotypes are due to transcriptional deficiencies. We also found more apoptotic cells in imaginal discs and in the CNS of hay mutant flies than in wild-type flies. Because this abnormal level of apoptosis was not detected in cdk7 flies, this phenotype could be related to defects in DNA repair. In addition the apoptosis induced by p53 Drosophila homolog (Dmp53) is suppressed in heterozygous hay flies.

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Synthesis of the major adult cuticle proteins of Drosophila melanogaster during hypoderm differentiation

Cited by 29 publications

References 18 publications

Ecdysone regulation of cuticle protein gene expression inDrosophila

Ecdysone regulation of cuticle protein gene expression inDrosophila

Genetic control of epidermis differentiation in Drosophila.

DNA Repair and Transcriptional Effects of Mutations in TFIIH inDrosophilaDevelopment

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