Abstract:The ADP‐glucose pyrophosphorylase small (S) and large (L) subunit genes from the unicellular algae Ostreococcus tauri were synthesized by PCR, subcloned, and the recombinant expression products characterized. This enzyme regulates the biosynthesis of starch in photosynthetic eukaryotes and has a reversible reaction in vitro. In higher plants, the L subunit makes the S subunit more sensitive to the activator. To understand the evolution of the subunit roles, we partially purified the S+L heterotetramer and S ho… Show more
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