Synthesis of the Ca2+-dependent cell adhesion molecule DdCAD-1 is regulated by multiple factors during Dictyostelium development

Yang, Chunzhong; Brar, Simuran K.; Desbarats, Laurie; Siu, Chi‐Hung

doi:10.1046/j.1432-0436.1997.6150275.x

Cited by 30 publications

(22 citation statements)

References 59 publications

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“…Dictyostelium cells form their initial contacts through interactions of a plasma membraneassociated Ca 2+ -dependent cadherin-like protein known as gp24 (DdCAD-1) (Yang et al, 1997). In early development this CAM is transported from the cytosol to the external surface of the plasma membrane and a small fraction of this CAM is released into the extracellular milieu.…”

Section: Introductionmentioning

confidence: 99%

Involvement of a Rab8-like protein of Dictyostelium discoideum, Sas1, in the formation of membrane extensions, secretion and adhesion during development

Powell

Temesvari

2004

Microbiology

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Establishment of cell–cell adhesions, regulation of actin, and secretion are critical during development. Rab8-like GTPases have been shown to modulate these cellular events, suggesting an involvement in developmental processes. To further elucidate the function of Rab8-like GTPases in a developmental context, a Rab8-related protein (Sas1) of Dictyostelium discoideum was examined, the expression of which increases at the onset of development. Dictyostelium cell lines expressing inactive (N128I mutant) and constitutively active (Q74L mutant) Sas1 as green fluorescent protein (GFP)-Sas1 chimeras were generated. Cells expressing Sas1Q74L displayed numerous actin-rich membrane protrusions, increased secretion, and were unable to complete development. In particular, these cells demonstrated a reduction in adhesion as well as in the levels of a cell adhesion molecule, gp24 (DdCAD-1). In contrast, cells expressing Sas1N128I exhibited increased cell–cell adhesion and increased levels of gp24. Counting factor is a multisubunit signalling complex that is secreted in early development and controls aggregate size by negatively regulating the levels of cell adhesion molecules, including gp24. Interestingly, the Sas1Q74L mutant demonstrated increased levels of extracellular countin, a subunit of counting factor, suggesting that Sas1 may regulate trafficking of counting factor components. Together, the data suggest that Sas1 may be a key regulator of actin, adhesion and secretion during development.

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Section: Introductionmentioning

confidence: 99%

Involvement of a Rab8-like protein of Dictyostelium discoideum, Sas1, in the formation of membrane extensions, secretion and adhesion during development

Powell

Temesvari

2004

Microbiology

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“…Dd-CAD-1 is a Ca 2+ -dependent cell adhesion molecule (Wong et al, 1996). Interestingly, growth conditions have a significant effect on the expression of Dd-CAD-1 (Yang et al, 1997). In addition, the three discoidin I genes are particularly well characterised as markers of the growth-development transition (under the hybridisation conditions used, the probe probably recognises the transcripts of all three discoidin 1 genes, so we will assume we are analysing their composite behaviour).…”

Section: Discussionmentioning

confidence: 99%

Dd-STATb, aDictyosteliumSTAT protein with a highly aberrant SH2 domain, functions as a regulator of gene expression during growth and early development

et al. 2004

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Dictyostelium, the only known non-metazoan organism to employ SH2 domain:phosphotyrosine signaling, possesses STATs (signal transducers and activators of transcription) and protein kinases with orthodox SH2 domains. Here, however, we describe a novel Dictyostelium STAT containing a remarkably divergent SH2 domain. Dd-STATb displays a 15 amino acid insertion in its SH2 domain and the conserved and essential arginine residue, which interacts with phosphotyrosine in all other known SH2 domains, is substituted by leucine. Despite these abnormalities, Dd-STATb is biologically functional. It has a subtle role in growth, so that Dd-STATb-null cells are gradually lost from the population when they are co-cultured with parental cells, and microarray analysis identified several genes that are either underexpressed or overexpressed in the Dd-STATb null strain. The best characterised of these,discoidin 1, is a marker of the growth-development transition and it is overexpressed during growth and early development of Dd-STATb null cells. Dimerisation of STAT proteins occurs by mutual SH2 domain:phosphotyrosine interactions and dimerisation triggers STAT nuclear accumulation. Despite its aberrant SH2 domain, the Dd-STATb protein sediments at the size expected for a homodimer and it is constitutively enriched in the nucleus. Moreover, these properties are retained when the predicted site of tyrosine phosphorylation is substituted by phenylalanine. These observations suggest a non-canonical mode of activation of Dd-STATb that does not rely on orthodox SH2 domain:phosphotyrosine interactions.

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“…To investigate how DdCAD-1 might be involved in cell sorting during development, cadA cDNA was fused to GFP and the fusion construct was ligated to the endogenous cadA promoter -631 (Yang et al, 1997) (see Fig. S3A in the supplementary material) for transfection into cadA -cells.…”

Section: Research Articlementioning

confidence: 99%

“…Mutations in these genes have major effects on aggregate formation and morphogenesis (Coates and Harwood, 2001;Siu et al, 2004). The Ca 2+ -dependent cell-cell contact sites are mediated by the adhesion molecule DdCAD-1 (also known previously as gp24), which is encoded by the cadA gene and expressed soon after the initiation of development (Brar and Siu, 1993;Wong et al, 1996;Wong et al, 2002;Yang et al, 1997). The Ca 2+ /Mg 2+ -independent contact sites are mediated by csA (gp80) (Harris and Siu, 2002;Kamboj et al, 1989;Siu et al, 1985;Stein and Gerisch, 1996) and TgrC1 (LagC, gp150) (Dynes et al, 1994;Gao et al, 1992;Wang et al, 2000), and they contribute to the formation of stable cell-cell adhesion during the aggregation phase and the post-aggregation phase, respectively.…”

Section: Introductionmentioning

confidence: 99%

The cell adhesion molecule DdCAD-1 regulates morphogenesis through differential spatiotemporal expression inDictyostelium discoideum

et al. 2011

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SUMMARYDuring development of Dictyostelium, multiple cell types are formed and undergo a coordinated series of morphogenetic movements guided by their adhesive properties and other cellular factors. DdCAD-1 is a unique homophilic cell adhesion molecule encoded by the cadA gene. It is synthesized in the cytoplasm and transported to the plasma membrane by contractile vacuoles. In chimeras developed on soil plates, DdCAD-1-expressing cells showed greater propensity to develop into spores than did cadA-null cells. When development was performed on non-nutrient agar, wild-type cells sorted from the cadA-null cells and moved to the anterior zone. They differentiated mostly into stalk cells and eventually died, whereas the cadA-null cells survived as spores. To assess the role of DdCAD-1 in this novel behavior of wild-type and mutant cells, cadA-null cells were rescued by the ectopic expression of DdCAD-1-GFP. Morphological studies have revealed major spatiotemporal changes in the subcellular distribution of DdCAD-1 during development. Whereas DdCAD-1 became internalized in most cells in the post-aggregation stages, it was prominent in the contact regions of anterior cells. Cell sorting was also restored in cadA -slugs by exogenous recombinant DdCAD-1. Remarkably, DdCAD-1 remained on the surface of anterior cells, whereas it was internalized in the posterior cells. Additionally, DdCAD-1-expressing cells migrated slower than cadA -cells and sorted to the anterior region of chimeric slugs. These results show that DdCAD-1 influences the sorting behavior of cells in slugs by its differential distribution on the prestalk and prespore cells.

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Synthesis of the Ca2+-dependent cell adhesion molecule DdCAD-1 is regulated by multiple factors during Dictyostelium development

Cited by 30 publications

References 59 publications

Involvement of a Rab8-like protein of Dictyostelium discoideum, Sas1, in the formation of membrane extensions, secretion and adhesion during development

Involvement of a Rab8-like protein of Dictyostelium discoideum, Sas1, in the formation of membrane extensions, secretion and adhesion during development

Dd-STATb, aDictyosteliumSTAT protein with a highly aberrant SH2 domain, functions as a regulator of gene expression during growth and early development

The cell adhesion molecule DdCAD-1 regulates morphogenesis through differential spatiotemporal expression inDictyostelium discoideum

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