Synthesis of tagatose and fucose from dairy raw material

Abstract

Innovative biochemical technologies have been developed for obtaining a fucose-containing carbohydrate complex and a tagatose-containing sweetener from the secondary dairy raw material, lactose. A method for the production of tagatose has been tested, including the enzymatic treatment of lactose Lactozym 6500 L Pure at pH = 6.2 for 4.5 hours and an enzyme dosage of 7500 LAU/dm3. The kinetic parameters of lactose hydrolysis were calculated: maximum rate and order of the chemical reaction (V max = 1.08 ⋅ 10-5, n = 0.6967). The qualitative and quantitative parameters of lactose hydrolysis and isomerization of galactose into tagatose, obtained by HPLC, are presented. The degree of lactose hydrolysis is 92%; the tagatose yield is 41%. A method for the production of fucose from lactose with subsequent conversion of galactose into fucitol and enzymatic isomerization into fucose is proposed. The fucose yield is 42.5% of the total solid mass.