Synthesis of synthetic mannan backbone polysaccharides found on the surface of Mycobacterium tuberculosis as a vaccine adjuvant and their immunological properties

Wattanasiri, Chakree; Paha, Jiraporn; Ponpuak, Marisa; Ruchirawat, Somsak; Boonyarattanakalin, Siwarutt

doi:10.1016/j.carbpol.2017.07.045

Cited by 16 publications

(9 citation statements)

References 36 publications

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“…Natural polysaccharide has the characteristics of intrinsic immune regulation, biocompatibility, biodegradation, low toxicity and safety, and has attracted much attention in the preparation of vaccines and adjuvants [ 7 , 10 , 31 ]. Moreover, it has been proved that a variety of natural polysaccharides possess better immune promoting effects through enhancing the effects of humoral, cellular and mucosal immunities [ 32 – 35 ].…”

Section: Discussionmentioning

confidence: 99%

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Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner

et al. 2020

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There is an urgent need for the development of new, improved vaccine adjuvants against T. spiralis infection. Polysaccharides are effective, safe, and biodegradable as adjuvant. In our study, we first observed the protective efficacy of lentinan as adjuvant against helminth T. spiralis infection. Recombinant T. spiralis Serpin (rTs-Serpin) immunoscreened from a cDNA library of T. spiralis, as a vaccine, protect host against Trichinella infection. The reduction rate of helminth burden of rTs-Serpin+lentinan-immunized mice was significantly increased compared with rTs-Serpin+FCA-immunized mice. rTs-Serpin+lentinan induced IgG1-dominant immune response and higher levels of IFN-γ and IL-4. rTs-Serpin+lentinan displayed a lower reduction rate of parasite burden in NLRP3-/mice than that in WT mice and lower level of IgG1 than that in WT mice. The level of IL-4, but not IFN-γ, from NLRP3-/mice immunized by rTs-Serpin+lentinan was significantly lower than that from WT mice, suggesting that NLRP3 is associated with rTs-Serpin+lentinan-triggering Th2 protective immunity against T. spiralis infection. In summary, we revealed that lentinan was a novel adjuvant against T. spiralis infection via NLRP3. NLRP3 therefore represents an important target for adjuvant discovery and the control of T. spiralis infection.

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Section: Discussionmentioning

confidence: 99%

“…Many polysaccharides from plant, bacterial, yeast and synthetic sources can act as pathogen-associated molecular patterns (PAMPs) and recognize pattern recognition receptors (PRRs) on immune cells, followed by regulating immunity [6][7][8][9]. Polysaccharides are safe, and biodegradable, with no tissue deposition [10].…”

Section: Introductionmentioning

confidence: 99%

Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner

et al. 2020

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“…Guo et al prepared several oligosaccharides as candidates for protein conjugation with decent biological activity and they concluded that the oligosaccharide part is important for the activity . Wattanasiri et al synthesized LAM mimetic polysaccharide using rapid synthetic approach and evaluated its immunological properties with positive results . Bavaro et al used monosaccharide, disaccharide and trisaccharide to conjugate the TB protein and all the three glycoproteins showed certain activity .…”

Section: Introductionmentioning

confidence: 99%

A Concise Synthesis of Oligosaccharides Derived From Lipoarabinomannan (LAM) with Glycosyl Donors Having a Nonparticipating Group at C2

Zheng

Terreni

et al. 2020

Eur J Org Chem

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Mycobacteria infection resulting in tuberculosis (TB) is one of the top ten leading causes of death over the world, and lipoarabinomannan (LAM) has been confirmed to play significant roles in this process. In this study, a convenient synthetic approach has been developed for the synthesis of oligosaccharides derived from LAM starting with commercially available substrates and reagents. The key steps for stereoselective construction of glycosidic bonds by acceptors glycosylated with donors without neighboring participating group were achieved.

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“…Immunological activities of the two synthetic conjugates were evaluated in mice showing that although both conjugates induced IgG antibody responses, the 6′-N-conjugate afforded higher quantity of antibody than the 1-O-conjugate [154,155] its immunological properties showing that the compound can enhance the secretion of TNF-α, IL-12, IL-6, and IL-1β. They suggested that the adjuvanticity mechanism of the compound involves the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and inflammasome pathways [156]. So, AMbased conjugates should be regarded as important forthcoming vaccine candidates in the future.…”

Section: Glycoconjugate Lam-based Vaccinesmentioning

confidence: 99%

Novel Vaccine Candidates against Tuberculosis

Zheng

Terreni

et al. 2020

CMC

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Ranking above AIDS, Tuberculosis (TB) is the ninth leading cause of death affecting and killing many individuals every year. Drugs’ efficacy is limited by a series of problems such as Multi- Drug Resistance (MDR) and Extensively-Drug Resistance (XDR). Meanwhile, the only licensed vaccine BCG (Bacillus Calmette-Guérin) existing for over 90 years is not effective enough. Consequently, it is essential to develop novel vaccines for TB prevention and immunotherapy. This paper provides an overall review of the TB prevalence, immune system response against TB and recent progress of TB vaccine research and development. Several vaccines in clinical trials are described as well as LAM-based candidates.

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Synthesis of synthetic mannan backbone polysaccharides found on the surface of Mycobacterium tuberculosis as a vaccine adjuvant and their immunological properties

Cited by 16 publications

References 36 publications

Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner

Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner

A Concise Synthesis of Oligosaccharides Derived From Lipoarabinomannan (LAM) with Glycosyl Donors Having a Nonparticipating Group at C2

Novel Vaccine Candidates against Tuberculosis

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