Synthesis of <sup>18</sup>F-Tetrafluoroborate via Radiofluorination of Boron Trifluoride and Evaluation in a Murine C6-Glioma Tumor Model

Jiang, Hongde; Bansal, Aditya; Pandey, Mukesh K.; Peng, Kah Whye; Suksanpaisan, Lukkana; Russell, Stephen J.; DeGrado, Timothy R.

doi:10.2967/jnumed.115.170894

Cited by 28 publications

(30 citation statements)

References 24 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Resolution can be improved further using high-resolution collimators (e.g. 0.25 mm) and at least 10-fold higher imaging sensitivity could be achieved using the PET tracer of NIS, 18 F-TFB, as demonstrated by our dexamethasone experiments 31 . Additionally, we were able to correlate our NIS/radiotracer imaging results with both histological and pathological analyses in the RC model.…”

Section: Discussionmentioning

confidence: 77%

New transgenic NIS reporter rats for longitudinal tracking of fibrogenesis by high-resolution imaging

Brunton

Suksanpaisan²,

Li³

et al. 2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

Fibrogenesis is the underlying mechanism of wound healing and repair. Animal models that enable longitudinal monitoring of fibrogenesis are needed to improve traditional tissue analysis post-mortem. Here, we generated transgenic reporter rats expressing the sodium iodide symporter (NIS) driven by the rat collagen type-1 alpha-1 (Col1α1) promoter and demonstrated that fibrogenesis can be visualized over time using SPECT or PET imaging following activation of NIS expression by rotator cuff (RC) injury. Radiotracer uptake was first detected in and around the injury site day 3 following surgery, increasing through day 7–14, and declining by day 21, revealing for the first time, the kinetics of Col1α1 promoter activity in situ. Differences in the intensity and duration of NIS expression/collagen promoter activation between individual RC injured Col1α1-hNIS rats were evident. Dexamethasone treatment delayed time to peak NIS signals, showing that modulation of fibrogenesis by a steroid can be imaged with exquisite sensitivity and resolution in living animals. NIS reporter rats would facilitate studies in physiological wound repair and pathological processes such as fibrosis and the development of anti-fibrotic drugs.

show abstract

Section: Discussionmentioning

confidence: 77%

New transgenic NIS reporter rats for longitudinal tracking of fibrogenesis by high-resolution imaging

Brunton

Suksanpaisan²,

Li³

et al. 2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, Khoshnevisan et al developed a method for synthesizing 18 F-TFB with high specific activity and sufficient yield [36]. Jiang et al also reported a new 18 F-TFB synthesis method which was achieved via direct radiofluorination of boron trifluoride using an automated synthesis system [37]. Using these new synthesis methods, high-specific-activity synthesis of 18 F-TFB was achieved.…”

Section: Discussionmentioning

confidence: 99%

Tracking dendritic cell migration into lymph nodes by using a novel PET probe 18F-tetrafluoroborate for sodium/iodide symporter

Lee

et al. 2017

EJNMMI Res

View full text Add to dashboard Cite

BackgroundRecently, 18F-tetrafluoroborate (TFB) was used as a substrate for the human sodium/iodide symporter (hNIS) reporter gene. This study evaluated the feasibility of performing molecular-genetic imaging by using the new radiotracer (18F-TFB) for the hNIS gene, to track dendritic cell (DC) migration in live mice. A murine dendritic cell line (DC2.4) co-expressing the hNIS and effluc genes (DC/NF) was established. To confirm the functional cellular expression of both effluc and NIS in the inoculated DC/NF cells by bio-medical imaging, combined bioluminescence imaging (BLI) and 18F-TFB positron emission tomography/computed tomography (PET/CT) imaging was performed after intramuscular injection with parental DCs and DC/NF cells. For DC-tracking, parental DCs or DC/NF cells were injected in the left or right mouse footpad, respectively, and 18F-TFB PET/CT and BLI were performed to monitor these cells in live mice.ResultsIn vivo PET/CT and BLI showed a clear signal in DC/NF injection sites but not in parental DC injection sites. The signal intensity in DC/NF cells was correlated with time. In vivo 18F-TFB PET/CT imaging showed higher radiotracer activity in the draining popliteal lymph nodes (DPLNs) in DC/NF injection sites than those in DC injection sites on day 2. BLI also showed DC/NF cell migration to the DPLNs on day 2 after the injection.ConclusionsMigration of DCs to the lymph nodes was successfully monitored using 18F-TFB PET/CT imaging of the NIS gene and optical imaging of the effluc gene in live mice. These data support the feasibility of using 18F-TFB as a substrate for hNIS reporter gene imaging to track the migration of DCs to the lymph nodes in live animals. The use of 18F-TFB may facilitate enhanced PET imaging of the hNIS reporter gene in small animals and humans in future studies.Electronic supplementary materialThe online version of this article (doi:10.1186/s13550-017-0280-5) contains supplementary material, which is available to authorized users.

show abstract

“…außerdem ein neues aliphatisches Trifluorborat, das durch benachbarte Ammoniumgruppen (in einer zwitterionischen Form) stabilisiert wurde, für die Synthese einer Reihe von klinisch relevanten Peptiden . Andere markierte Moleküle, die mittels B‐ 18 F‐Bindungsbildung erhalten wurden, konnten bei der Synthese von [ 18 F]BODIPY, NHC‐[ 18 F]BF 3 ‐Addukt und [ 18 F]Tetrafluorborat durch Halogen‐ 18 F‐Austausch nachgewiesen werden . Kürzlich veröffentlichten Chen, Liu et al.…”

Section: Markierungsmethoden Mit Fluor‐18unclassified

Chemie der Positronenemissionstomographie: Aktuelle Fortschritte bei ¹¹C‐, ¹⁸F‐, ¹³N‐ und ¹⁵O‐Markierungsreaktionen

et al. 2019

View full text Add to dashboard Cite

Die Positronenemissionstomographie (PET) ist ein molekulares bildgebendes Verfahren, das in vivo quantitative Informationen über Funktion und Metabolismus bei biologischen Prozessen für die Diagnose von Krankheiten und die Therapiebewertung liefert. Die breiten Anwendungsmöglichkeiten und die schnellen Fortschritte bei der PET haben zu einem ansteigenden Bedarf an neuen radiochemischen Methoden zur Synthese hochspezifischer Moleküle mit positronenemittierenden Radionukliden geführt. Dieser Aufsatz gibt einen Überblick über die gängige verwendete Markierungschemie in Bezug auf Beispiele klinisch relevanter PET‐Tracer und zeigt die jüngsten Entwicklungen und bahnbrechenden Erfolge im letzten Jahrzehnt, wobei der Schwerpunkt auf 11C, 18F, 13N und 15O liegt.

show abstract

Synthesis of ¹⁸F-Tetrafluoroborate via Radiofluorination of Boron Trifluoride and Evaluation in a Murine C6-Glioma Tumor Model

Cited by 28 publications

References 24 publications

New transgenic NIS reporter rats for longitudinal tracking of fibrogenesis by high-resolution imaging

New transgenic NIS reporter rats for longitudinal tracking of fibrogenesis by high-resolution imaging

Tracking dendritic cell migration into lymph nodes by using a novel PET probe 18F-tetrafluoroborate for sodium/iodide symporter

Chemie der Positronenemissionstomographie: Aktuelle Fortschritte bei ¹¹C‐, ¹⁸F‐, ¹³N‐ und ¹⁵O‐Markierungsreaktionen

Contact Info

Product

Resources

About

Synthesis of 18F-Tetrafluoroborate via Radiofluorination of Boron Trifluoride and Evaluation in a Murine C6-Glioma Tumor Model

Cited by 28 publications

References 24 publications

New transgenic NIS reporter rats for longitudinal tracking of fibrogenesis by high-resolution imaging

New transgenic NIS reporter rats for longitudinal tracking of fibrogenesis by high-resolution imaging

Tracking dendritic cell migration into lymph nodes by using a novel PET probe 18F-tetrafluoroborate for sodium/iodide symporter

Chemie der Positronenemissionstomographie: Aktuelle Fortschritte bei 11C‐, 18F‐, 13N‐ und 15O‐Markierungsreaktionen

Contact Info

Product

Resources

About

Synthesis of ¹⁸F-Tetrafluoroborate via Radiofluorination of Boron Trifluoride and Evaluation in a Murine C6-Glioma Tumor Model

Chemie der Positronenemissionstomographie: Aktuelle Fortschritte bei ¹¹C‐, ¹⁸F‐, ¹³N‐ und ¹⁵O‐Markierungsreaktionen