Synthesis of Silver Nanoparticles with Tunable Morphologies via a Reverse Nano-Emulsion Route

Feng, Anni; Wu, Shuang; Chen, Sen-Yuan; Zhang, Huan; Shao, Wenyao; Xiao, Zongyuan

doi:10.2320/matertrans.m2013005

Cited by 8 publications

(4 citation statements)

References 27 publications

(15 reference statements)

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“…Moreover, these materials have a wide range of applications: detectors, catalysts, surface coating agents, and antibacterial/antimicrobials, among many others. Some of the most studied metallic nanoparticles include silver (Ag) [2, 3], gold (Au) [4], platinum (Pt) [5–7], and palladium (Pd) [8].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Silver Nanoparticles Using Aqueous Extract of Medicinal Plants’ (Impatiens balsaminaandLantana camara) Fresh Leaves and Analysis of Antimicrobial Activity

Aritonang

Koleangan

Wuntu

2019

International Journal of Microbiology

164

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Plant-mediated synthesis of nanomaterials has been increasingly gaining popularity due to its eco-friendly nature and cost-effectiveness. In the present study, we synthesized silver (Ag) nanoparticles using aqueous extracts of fresh leaves of Impatiens balsamina and Lantana camara medicinal plants as bioreducing agents. This method allowed the synthesis of nanoparticles, which was confirmed by ultraviolet-visible (UV-Vis) spectrophotometry and transmission electron microscopy (TEM). UV-Vis spectra and visual observation showed that the color of the fresh leaf extracts of L. camara and I. balsamina turned into grayish brown and brownish yellow, respectively, after treatment with Ag precursors. In addition, TEM analysis confirmed that AgNO3 solutions for all concentrations produced Ag nanoparticles and their average size was less than 24 nm. Moreover, aqueous leaf extracts of I. balsamina and L. camara were separately tested for their antimicrobial activity against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria. The results showed that the bacterial growth was inhibited by the extracts containing Ag nanoparticles. Statistical calculation performed using the Tukey test showed that zones of inhibition for the two bacteria produced by the aqueous leaf extracts of L. camara containing 3 mM and 5 mM Ag precursors were not significantly different from that by ciprofloxacin as positive control. On the contrary, there was significant difference between the zone of inhibition for E. coli by ciprofloxacin and that by the extracts of I. balsamina leaves containing 3 mM and 5 mM Ag precursors. A similar result was observed on the zone of inhibition for S. aureus by the extracts of I. balsamina leaves containing 3 mM Ag precursor. It was shown that the aqueous extracts of fresh L. camara leaves containing Ag nanoparticles were comparable to ciprofloxacin in inhibiting bacterial growth.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Silver Nanoparticles Using Aqueous Extract of Medicinal Plants’ (Impatiens balsaminaandLantana camara) Fresh Leaves and Analysis of Antimicrobial Activity

Aritonang

Koleangan

Wuntu

2019

International Journal of Microbiology

164

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“…Furthermore, these nanoparticles have a varied range of usage in various fields, including food and pharmaceutical industries, detectors, catalysts, antibacterials, antimicrobials, and surface-coating agents. There are several metallic NPs that have been extensively studied, such as silver (Ag) [33,34] platinum (Pt) [35], gold (Au) [36], and palladium (Pd). The study of AgNPs in the areas of health and science is of particular interest.…”

Section: Discussionmentioning

confidence: 99%

Biosynthesized Silver Nanoparticles from Eruca sativa Miller Leaf Extract Exhibits Antibacterial, Antioxidant, Anti-Quorum-Sensing, Antibiofilm, and Anti-Metastatic Activities

et al. 2022

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Worldwide, the primary problem today is the proliferation of cancer and secondary bacterial infections caused by biofilms, as they are the principal causes of death due to the lack of effective drugs. A great deal of biological activities of silver nanoparticles (AgNPs) have made them a brilliant choice for the development of new drugs in recent years. The present study was conducted to evaluate the anticancer, antibacterial, anti-QS, and antibiofilm effects of AgNPs synthesized from Eruca sativa (E. sativa) leaf extract. The ultraviolet–visible (UV–Vis) spectra showed a peak of surface plasmon resonance at 424 nm λmax, which corresponded to AgNP formation. The Fourier transform infrared spectroscopy (FT-IR) confirmed that biological moieties are involved for the development of AgNPs. Moreover, transmission electron microscopy (TEM) analyses confirmed the spherical shape and uniform size (8.11 to 15 nm) of the AgNPs. In human lung cancer cells (A549), the anticancer potential of AgNPs was examined by the MTT [3-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, scratch assay, and invasion assay. The results indicated that AgNPs inhibit the migration of A549 cells. The synthesized AgNPs showed MIC values of 12.5 µg/mL against Chromobacterium violaceum (C. violaceum) and 25 µg/mL against Pseudomonas aeruginosa (P. aeruginosa), which demonstrated their antibacterial abilities. Biological compounds that disable the QS system are being investigated as potential strategies for preventing bacterial infections. Thus, we analyzed the potential effectiveness of synthesized AgNPs in inhibiting QS-regulated virulence factors and biofilm formation in both strains of bacteria. In C. violaceum, the synthesized AgNPs significantly inhibited both violacein (85.18% at 1/2 × MIC) and acyl homoserine lactone (78.76% at 1/2 × MIC). QS inhibitory activity was also demonstrated in P. aeruginosa at a sub-MIC concentration (1/2 × MIC) by a reduction in pyocyanin activity (68.83%), total protease (68.50%), LasA activity (63.91%), and LasB activity (56.40%). Additionally, the exopolysaccharide production was significantly reduced in both C. violaceum (65.79% at 1/2 × MIC) and P. aeruginosa (57.65% at 1/2 × MIC). The formation of biofilm was also significantly inhibited at 1/2×MIC in C. violaceum (76.49%) and in P. aeruginosa (65.31%). Moreover, a GC–MS analysis confirmed the presence of different classes of bioactive phytochemical constituents present in the leaf extract of E. sativa. On the basis of our results, we conclude that biologically synthesized AgNPs showed numerous multifunctional properties and have the potential to be used against human cancer and bacterial biofilm-related infections.

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“…Different shapes of silver nanoparticles could be fabricated, such as, discs, rods, wires, spheres, prisms, right bipyramids, stars, and cubes. Many applications were reported to different shapes of silver nanoparticles including electronics, surface-enhanced Raman scattering (SERS), catalysis, and as antimicrobial sterilization to minimize toxicity toward mammalian cells (Feng et al, 2013;Ijaz Hussain et al, 2011). Various techniques have been reported previously for silver nanoparticles preparation, including chemical, physical, and biological methods.…”

Section: Introductionmentioning

confidence: 99%

Silver Nanoparticles as Potential Antibacterial and Antibiofilm Agents: Insights from Experimental and Computational Approaches

Alhadrami,

Elswifi,

Rateb

et al. 2023

Preprint

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Background: In the present study we prepared silver nanoparticles (AgNPs) by the chemical reduction method which is considered more preferable than other previously reported methods. The prepared AgNPs were further evaluated for their antibacterial properties and their mode of action was putatively elucidated by molecular dynamics (MD) simulations. Results: The structure and uniformity of the prepared AgNPs was confirmed by different characterization techniques including XRD, SEM, FT-IR, UV-vis spectroscopy, zeta potential, and DLS. Testing these AgNPs for their antibacterial and antibiofilm activities revealed very good potential against E. coli and K. pneumoniae (MIC = 37.5 and 18.75 μg/mL, respectively), P. aurigenosa and C. albicans (Percent of biofilm inhibition = 43% and 50%, respectively). Further comprehensive MD simulations revealed that the desorbed Ag atoms can bind with and probably inhibit the bacterial GSH reductase resulting in fatal increased oxidative stress inside the bacterial cell. Conclusions: AgNPS produced by chemical methods are easy to prepare nanomaterial that exert considerable antibacterial effects via the inhibition of the bacterial GSH reductase. To the best of our knowledge, this is the first comprehensive MD simulation work that uncover the mechanism of the bacterial GSH reductase inhibition by Ag atoms. Accordingly, we believe that the results presented herein will pave the way for additional research into metal-protein interactions, which will aid in the development of new metal-based treatments.

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Synthesis of Silver Nanoparticles with Tunable Morphologies via a Reverse Nano-Emulsion Route

Cited by 8 publications

References 27 publications

Synthesis of Silver Nanoparticles Using Aqueous Extract of Medicinal Plants’ (Impatiens balsaminaandLantana camara) Fresh Leaves and Analysis of Antimicrobial Activity

Synthesis of Silver Nanoparticles Using Aqueous Extract of Medicinal Plants’ (Impatiens balsaminaandLantana camara) Fresh Leaves and Analysis of Antimicrobial Activity

Biosynthesized Silver Nanoparticles from Eruca sativa Miller Leaf Extract Exhibits Antibacterial, Antioxidant, Anti-Quorum-Sensing, Antibiofilm, and Anti-Metastatic Activities

Silver Nanoparticles as Potential Antibacterial and Antibiofilm Agents: Insights from Experimental and Computational Approaches

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