Synthesis of Silver Nanoparticles Using L.Rosa Flowers Extracts: Thermodynamic and Kinetic Studies on the Inhibitoty Effects of Nanoparticles on Creatine Kinase Activity

Abstract

The present work investigates the synthesis of silver nanoparticles (AgNPs) by a biological method using L.Rosa flower extract and silver nitrate as precursors. Optimum conditions of synthesis were studies, such as pH, temperature, concentration of extract, concentration of silver nitrate, and stability with time. Characterization of AgNPs was carried out using UV-visible Spectroscopy, Scanning Electron Microscopy, X-Ray Diffraction, Fourier Transform Infrared and Transmission Electron Microscopy. The biosynthesized AgNPs exhibited inhibitory effects on creatine kinase activity in the sera of patients with myocardial infarction, compared with control subjects. Thermodynamic and kinetic studies of creatine kinase were performed. Further studies on other biological activities were performed to exploit AgNPs full potential. In conclusions, the present study utilize a simple, cheap and environmentally green method to synthesize silver nanoparticles. This single step procedure is more suitable for large scale production as it is rapid and eliminates the elaborate processes employed in the other bio-based protocols (e.g. by using fungi and bacteria).

Green Synthesis of Nanocomposite: Based on [Eugenol and Metal Oxides], Characterization and Biomedical Applications

“…Due to their size, stability, high surface area, catalytic activity, simplicity in fabrication, and selectivity for particular reactions 14,15 , (MONCs) have been used in a variety of chemical reactions, including coupling reactions, electrochemical reactions, as well as oxidation and reduction reactions 15 . (MONCs) with various morphologies have been created by physical, chemical, and biological processes 9,14 , such as solgel, hydrothermal, co-precipitation, thermal decomposition, microemulsion, solvothermal, microwave-assisted, and sonochemical 16 . In our research, we employed a sol-gel preparation approach.…”

Investigating the Antioxidant and Apoptosis Inducing Effects of Biologically Synthesized Silver Nanoparticles Against Lymphoma Cells in Vitro

“…Physical and chemical approaches involve the use of toxic chemicals that may lead to hazardous consequences like toxic, carcinogenic, and environmentally harmful effects [14]. The biological approach, or green chemical approach, deals with the usage of biological entities, parts of them, or their products as reducing and stabilizing factors in the nanoparticle synthesis process, thus offering a clean, reliable, and eco-friendly method for approaching [15]. Programmed cell death, or apoptosis, is a key mechanism for homeostasis; it is responsible for eliminating aged cells as well as damaged cells, virally infected cells, and cancer cells [16].…”