Synthesis of silver nanoparticles from Bacillus brevis (NCIM 2533) and their antibacterial activity against pathogenic bacteria

Saravanan, Muthupandian; Barik, Sisir Kumar; MubarakAli, Davoodbasha; Prakash, P.; Pugazhendhi, Arivalagan

doi:10.1016/j.micpath.2018.01.038

Cited by 327 publications

(122 citation statements)

References 40 publications

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“…[1][2][3] Among the antibiotic nanomaterials, silver nanoparticles (AgNPs) were endowed to more different fields in our daily life, including textiles, sanitary articles, and cosmetics, because of their excellent broad-spectrum antimicrobial properties. [3][4][5][6][7][8][9] There have been many biomedical products containing AgNPs on the market to date, such as wound dressings, catheters, bone cement, and artificial cardiac valves. 10,11 With the growing usage of nanosilver-containing products, people are more likely to be exposed to AgNPs.…”

Section: Introductionmentioning

confidence: 99%

Size-dependent cellular uptake and localization profiles of silver nanoparticles

Guo

Liu

et al. 2019

IJN

213

118

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Purpose: Silver nanoparticles (AgNPs) have been widely applied in various fields as excellent antibacterial reagents over the past decades. Although the particle size is considered as the most crucial factor influencing cellular uptake, transportation, and accumulation behaviors, there are still many controversies regarding the correlation between size and uptake of AgNPs. In this study, size-dependent cellular uptake of AgNPs with different diameters was investigated in B16 cells. Methods: The uptake of AgNPs was investigated by inductively coupled plasma-mass spectrometry (ICP-MS) and transmission electron microscopic (TEM) imaging in B16 cells. Results: Twenty nanometer and 100 nm AgNPs had the lowest and highest uptake efficiency at both 12 hours and 24 hours, respectively. Smaller AgNPs crossed the plasma membrane faster with uniform distribution: 5 nm AgNPs were detected in both cytoplasm and nucleus at 0.5 hours after incubation. Larger AgNPs were extremely difficult to migrate: 100 nm AgNPs were detected in the nucleus at 12 hours after incubation. Internalization of AgNPs was directly observed, mainly within membrane-bound structures, such as intracellular vesicles and late endosomes. The uptake of all four-sized AgNPs (5 nm, 20 nm, 50 nm, 100 nm) decreased significantly after the pre-treatment with chlorpromazine hydrochloride, which can specifically inhibit the clathrin-mediated endocytosis. The internalization efficiencies of AgNPs (5 nm, 20 nm, 50 nm) were markedly reduced by methyl-β-cyclodextrin, a specific caveolin-mediated endocytosis inhibitor, whereas 5-(N-ethyl-N-isopropyl) amiloride as an inhibitor of macropinocytosis inhibited the uptake of larger sizes of AgNPs (50 nm and 100 nm). Conclusion: The results suggest that the size of AgNPs can not only affect the efficiency of cellular uptake, but also the type of endocytosis. The clathrin-mediated endocytosis may be the most common endocytic pathway for AgNPs in B16 cells, and AgNPs at each size were likely to enter cells by a major internalization pathway.

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Section: Introductionmentioning

confidence: 99%

Size-dependent cellular uptake and localization profiles of silver nanoparticles

Guo

Liu

et al. 2019

IJN

213

118

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“…towards AgNPs has many exceptions, thus, AgNPS synthesized from Bacillus brevis has a maximum antibacterial effect against the Gram-positive, multi-drug resistant for Staphylococcus aureus and moderate for the Gram-negative Salmonella typhi. 148 Role of Ag in Particle State (Ag 0 ) and Ag + Species in Mediating the Bactericidal Effect of Silver Nanoparticles Ag in Particle State (Ag 0 ) This is the first (direct or "primary"), of antibacterial effect of AgNPs and is considered to be due to: (1) nanoparticles damage the bacterial wall and on (2) entrance of particles into the bacterial cytosol and directly interact with the intrabacterial environment. [149][150][151] The adherence of the AgNPs on the bacterial surface and formation of particle agglomerates is followed by disruption of bacterial membrane integrity by induction of cell-wall pits and gaps, and alteration in membrane selectivity and permeability, including ionic transport.…”

Section: Antibacterial Effect and Mechanism Of Silver Nanoparticlesmentioning

confidence: 99%

Silver Nanoparticles for the Therapy of Tuberculosis

Tabaran

Matea²,

Mocan³

et al. 2020

IJN

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Rapid emergence of aggressive, multidrug-resistant Mycobacteria strain represents the main cause of the current antimycobacterial-drug crisis and status of tuberculosis (TB) as a major global health problem. The relatively low-output of newly approved antibiotics contributes to the current orientation of research towards alternative antibacterial molecules such as advanced materials. Nanotechnology and nanoparticle research offers several exciting new-concepts and strategies which may prove to be valuable tools in improving the TB therapy. A new paradigm in antituberculous therapy using silver nanoparticles has the potential to overcome the medical limitations imposed in TB treatment by the drug resistance which is commonly reported for most of the current organic antibiotics. There is no doubt that AgNPs are promising future therapeutics for the medication of mycobacterial-induced diseases but the viability of this complementary strategy depends on overcoming several critical therapeutic issues as, poor delivery, variable intramacrophagic antimycobacterial efficiency, and residual toxicity. In this paper, we provide an overview of the pathology of mycobacterial-induced diseases, andhighlight the advantages and limitations of silver nanoparticles (AgNPs) in TB treatment.

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“…The potent antibacterial efficacy of ANB-AgNPs was investigated using disc diffusion assay against E. coli and multi-drug resistant P. aeruginosa on LB agar plates (Saravanan et al, 2018). In both the cases, the antibacterial activity was evaluated by employing assay for stipulated concentrations (0.25, 0.5, 0.75 and 1 µg) of AgNPs on discs (C, D, E, F).…”

Section: Evaluation Of Antibacterial Activity Using Disc Diffusion Assaymentioning

confidence: 99%

“…Along with this, silver nanoparticles synthesized through natural products have shown potent antioxidant (Ali et al, 2012) and catalytic properties (Balakumar et al, 2017;Vellaichamy and Periakaruppan, 2016 a, c). Silver nanoparticles are also known for their wound healing properties specifically green synthesized silver nanopar-mari et al, 2016;Lukman et al, 2011;Narayanan and Sakthivel, 2011;Saravanan et al, 2018;Sathishkumar et al, 2009;Singh et al, 2016a, b;Vellaichamy and Periakaruppan, 2016b).…”

Section: Introductionmentioning

confidence: 99%

Versatile biomedical potential of biosynthesized silver nanoparticles from Acacia nilotica bark

Arya¹,

Kumari²,

Pundir³

et al. 2019

J Appl Biomed

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The present study reports the development of potent silver nanoparticles (AgNPs) using bark extract of Acacia nilotica and evaluation of its wound healing, anti-biofilm, anti-cancer and anti-microbial activity. Stable, small sized nanoparticles with spherical morphology were obtained after significant optimization studies that was evaluated through UV-visible spectroscopy. Thereafter, physicochemical characterization of biosynthesized AgNPs was carried out through DLS and FESEM for evaluation of size. EDAX and FTIR were carried out for the evaluation of composition and possible functional groups involved in the reduction and capping of AgNPs. The antibacterial potential was investigated through disc diffusion assay against Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa). Further, the Congo Red Assay (CRA) successfully revealed the anti-biofilm activity against Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Proteus vulgaris (P. vulgaris), Pseudomonas aeruginosa (P. aeruginosa). Alamar blue assay was conducted in A549 cells to reveal the remarkable anticancer activity of biosynthesized AgNPs that resulted in a very appreciable manner. Further, the wound healing activity of AgNPs can heal the excised wound of mice up-to 100% within 15 days. All these studies suggested that our biosynthesized AgNPs possess versatile biomedical application. Highlights:• Development of potent silver nanoparticles using bark extract of Acacia nilotica.• Characterized the ANB-AgNPs via UV, DLS, FTIR, FESEM and EDAX. • Evaluated of antibacterial and antibiofilm potential of ANB-AgNPs. • Evaluated the anticancer and wound healing potential of ANB-AgNPs.

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Synthesis of silver nanoparticles from Bacillus brevis (NCIM 2533) and their antibacterial activity against pathogenic bacteria

Cited by 327 publications

References 40 publications

<p>Size-dependent cellular uptake and localization profiles of silver nanoparticles</p>

<p>Size-dependent cellular uptake and localization profiles of silver nanoparticles</p>

<p>Silver Nanoparticles for the Therapy of Tuberculosis</p>

Versatile biomedical potential of biosynthesized silver nanoparticles from Acacia nilotica bark

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