Synthesis of silver nanoparticles from Turbinaria ornata and its antibacterial activity against water contaminating bacteria

Anuluxan, Santhiran; Thavaranjit, A. C.; Prabagar, Subramaniam; Silva, R. Chinthaka L. De; Prabagar, Jasotha

doi:10.1007/s11696-021-02033-8

Cited by 3 publications

(2 citation statements)

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“…Few reports are published on the synthesis of Sn-O 2 and Mg(OH) 2 NPs from T. ornata extract has been reported with antibacterial activity (Govindaraju et al, 2020;Sunny et al, 2022). In a study by Anuluxan et al (2022) using AgNPs derived from T. ornata against water-contaminated bacteria, Gram-positive S. aureus, B. circulans, and E. faecalis and Gram-negative E. coli and P. aeruginosa and found that To-AgNPs demonstrated the strongest antibacterial activity against S. aureus, which was consistent with our findings.…”

Section: Anti-oxidant and Anti-uropathogenic Analysis Of To-agnpsmentioning

confidence: 99%

“…Additionally, bioactive compounds in T. ornata extract bind and stabilize AgNPs, making them more effective against microbes. Several studies have demonstrated the antibacterial effect of AgNPs synthesized from T. ornata against biofilm microbes ( Krishnan et al, 2015 ) and water-contaminating bacteria ( Anuluxan et al, 2022 ). To our knowledge, there have been no reports on T. ornata -mediated AgNPs as an uropathogen target.…”

Section: Introductionmentioning

confidence: 99%

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Structural characterization, antioxidant and anti-uropathogenic potential of biogenic silver nanoparticles using brown seaweed Turbinaria ornata

Raj,

Muthukumar,

Dahms

et al. 2023

Front. Microbiol.

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Alternative treatment strategies for urinary tract infections (UTIs) are becoming more necessary due to increasing drug resistance patterns in uropathogens. Nanoparticle-based therapeutics is emerging as a way to treat UTIs. In the present study, using Turbinaria ornata extract, silver nanoparticles (AgNPs) were synthesized, characterized, and their anti-uropathogenic activity was evaluated. The stability and formation of synthesized To-AgNPs were confirmed by UV-visible spectroscopy, FTIR, XRD, SEM, and DLS. An FTIR spectrum confirmed the presence of seaweed functional groups in To-AgNPs, a XRD analysis confirmed their crystalline nature, and SEM imaging confirmed their spherical nature with an average size of 73.98 nm with diameters ranging from 64.67 to 81.28 nm. This was confirmed by TEM results. DLS determined that the cumulant hydrodynamic diameter of To-AgNPs was 128.3 nm with a PdI of 0.313 and the zeta potential value were found to be –63.3 mV which indicates the To-AgNPs are negatively charged and more stable. DPPH assays were used to assess the antioxidant activity of biosynthesized To-AgNPs, while an agar well diffusion method was used to test the antibacterial activity against uropathogens, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, and Klebsiella pneumoniae. The To-AgNPs showed the highest susceptibility to S. aureus (15.75 ± 0.35 mm) and E. coli (15 ± 0.7 mm) with MIC values of 0.0625 and 0.125 mg/ml, respectively in macro broth dilution method and observed considerable membrane damage under CLSM and SEM. To-AgNPs displayed stronger antioxidant and antimicrobial activity, suggesting they may be developed as a new class of antimicrobial agents for treating UTIs.

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Section: Anti-oxidant and Anti-uropathogenic Analysis Of To-agnpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%