Synthesis of scaffold-free, three dimensional, osteogenic constructs following culture of skeletal osteoprogenitor cells on glass surfaces

Non-additive effect of the DNA methylation inhibitor, 5-Aza-dC, and glass as a culture surface on osteogenic differentiation

“…MG-63 cell line is commonly used for modeling MSCs, based on their multilineage differentiation ability [ 11 , 13 , 14 , 15 , 16 ], purchased from ATCC (Manassas, VA, USA). MG-63 cells were cultured in basal media consisting of Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal calf serum and penicillin/streptomycin until 50% confluency.…”

“…Recently, we reported the positive effect of glass as a cell culture surface on MSCs differentiation into the osteogenic lineage. The cells showed enhanced proliferation and differentiation and formed a self-assembly three-dimensional (3D) construct rich in the osteogenic matrix [ 11 ]. Glass, through inducing histone methylation (H3K4), protects genes from permanent silencing by repelling transcriptional repressors and blocking DNA methylation [ 12 ].…”

Biomaterials as a Vital Frontier for Stem Cell-Based Tissue Regeneration

“…On the other hand, self-assembly bone construct can be associated with spontaneous chondrogenic matrix formation and the extension of capillary like structure. The biomaterial used in this case was glass, which served as a surface to allow folding of the monolayer in a 3D bony construct ( Alghfeli et al, 2021 ).…”

Promise and Perspective of Nanomaterials in Antisenescence Tissue Engineering Applications

“…The interaction between cell and biomaterial is facilitated through cell receptors, including cell-matrix adhesion molecules, providing a direct connection between extracellular microenvironmental factors (e.g., ECM proteins) and intracellular machinery. Integrins, one major group of cellular receptors, have been shown to mediate the interactions between cells and ECM molecules, playing a critical role in controlling stem cell fates into different lineage differentiation. , Recent studies suggested the involvement of integrins in cell senescence pathogenesis; − notably, a strong association is found between β3 integrin levels and aging in a subset of tissues . The outside-in signal transductions are essential for providing appropriate signals to cells and tissues, controlling the orientation and localization of subcellular organelle, and subsequent cell behaviors and functionalities. , Therefore, there is a need for the rational design and fabrication of next-generation biomaterials capable of rendering an antisenescence and pro-regenerative microenvironment by controlling the cell senescent phenotype with proper biomaterial-cell interactions.…”

“…Integrins, one major group of cellular receptors, have been shown to mediate the interactions between cells and ECM molecules, playing a critical role in controlling stem cell fates into different lineage differentiation. 60,61 Recent studies suggested the involvement of integrins in cell senescence pathogenesis; 62−64 notably, a strong association is found between β3 integrin levels and aging in a subset of tissues. 65 The outside-in signal transductions are essential for providing appropriate signals to cells and tissues, controlling the orientation and localization of subcellular organelle, and subsequent cell behaviors and functionalities.…”