Synthesis of Sarcophaga Lectin and Sarcotoxins in Nih-Sape-4, an Embryonic Cell Line of Sarcophaga Peregrina

Romano, H M; Kasama, Emi; Nakanishi, Yoko; Matsuyama, Kenji; Ando, Kentaro; Nagasawa, Yasuo; Natori, Shunji

doi:10.1007/978-3-642-73626-1_19

Cited by 4 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the intact insect, these molecules are secreted by diverse cell types, including fat body, haemocytes and midgut (Kanost et al, 1990;Hultmark, 1993;Homann, 1997;Lehane et al, 1997). Some of these immunity proteins are also synthesized by insect cell lines, as has been shown in early studies with the NIH-Sape-4 line of Sarcophaga peregrina (Komano et al, 1988), and in more recent studies with cell lines from Drosophila melanogaster (Samakovlis et al, 1992) and from the mosquitoes Aedes albopictus and Aedes aegypti (Hernandez et al, 1994;Yoshiga et al, 1997;Sun et al, 1998) and Anopheles gambiae (Dimopoulos et al, 1997). Although the combination(s) of factors produced by any individual cell line remain to be fully documented, it seems reasonable to anticipate that inducible cell lines will eventually provide useful in vitro models for examining selected aspects of the immune responses elicited by the challenge of an intact insect.…”

Section: Introductionmentioning

confidence: 91%

Immunity proteins from mosquito cell lines include three defensin A isoforms from Aedesaegypti and a defensin D from Aedesalbopictus

Gao¹,

Hernandez²,

Fallon³

1999

Insect Molecular Biology

View full text Add to dashboard Cite

An Aedes aegypti mosquito cell line, Aag-2, exhibits a response to immune stimulation that is qualitatively similar to that of C7-10 cultured cells from the related mosquito, Aedes albopictus. Using SDS polyacrylamide gels, we found that a small peptide was preferentially induced by the treatment of growing cells with heat-killed, Gram-positive bacteria. By an analogy with other studies, this small peptide was postulated to be a member of the defensin family of insect immunity peptides. A differential display was used to obtain partial polymerase chain reaction products corresponding to mRNAs that were preferentially expressed in induced cells. One of these products, which contained the partial sequence of a defensin gene, was used to screen cDNA libraries from Ae. aegypti and Ae. albopictus cells. From Ae. aegypti cells, we found two previously described isoforms (A1 and A4) of mosquito defensin A, as well as a new isoform which we defined as A5. From Ae. albopictus cells, we found a new mature mosquito defensin, named D, which contains proline and isoleucine as the final amino acids. In both Ae. aegypti and Ae. albopictus cell lines, the expression of defensin mRNA was visible on Northern blots as early as 3 h after exposure to heat-killed bacteria, and defensin mRNA abundance was maximal at 12-36 h after induction.

show abstract

Section: Introductionmentioning

confidence: 91%

Immunity proteins from mosquito cell lines include three defensin A isoforms from Aedesaegypti and a defensin D from Aedesalbopictus

Gao¹,

Hernandez²,

Fallon³

1999

Insect Molecular Biology

View full text Add to dashboard Cite

show abstract

“…The primary culture of normal pituitary cells of carp (Cyprinus carpio) released gonadropin during in vitro culture (Ribeiro et al, 1983). Similarly, synthesis of Sarcophaga lectin and sarcotoxins in NIH-SAPE-4 from the Sarcophaga peregrine embryonic cell line was also reported by the Komano et al (1988). The parasitic adhesions on host surfaces have been widely employed in mammalian cell lines (Lumb et al, 1988).…”

Section: Introductionmentioning

confidence: 73%

In vitro CULTURE OF FISH LOUSE (Lepeoptheirus salmonis) IN ATLANTIC SALMON (Salmo salar L. ) HOST CELL LINE

Devadason¹

2018

JEBAS

View full text Add to dashboard Cite

The salmon louse (Lepeophtherius salmonis), is a crustacean ectoparasite of salmonids and causes significant economical losses in Atlantic salmon farms. The aim of this study was establishing the in vitro culture and explants of infective stage of L.salmoins. The skin epithelial cell line of the Atlantic salmon was made and the sterilized chalimus stage was added for attachment. An experimentally was tried to produce the cell culture of the chalimus using growth medium. Chalimus attachment on Atlantic salmon fibroblast and epithelial cell line was observed. The supernatant which was collected from the chalimus attached wells screened by using SDS-PAGE, showed the protein (60kd) band which have been not reported in control without chalimus attached. The chalimus attachment was observed in ASE-W cell line but not CHSE-214 and RTG cell line which showed that Atlantic salmon was more susceptible for L. salmonis infection and this infection was species specific. The chalimus cell culture showed no growth in the growth medium used. This study presents a novel approach to cultivate the infective stages of sea lice, the copepod did and the attached stage, the chalimus in vitro.

show abstract

“…Previously we purified Sarcophaga lectin (20) and sapecin (28) from the culture medium of NIH-Sape-4 cells, a cell line derived from a Sarcophaga embryo. Since Northern blotting experiments revealed that both the Sarcophaga lectin gene (49) and the sapecin gene (29) (44,47).…”

Section: Discussionmentioning

confidence: 99%

“…The embryonic cell line NIHSape-4 from the flesh fly S. peregnna was cultured in M-M medium at 25°C as previously described (20). Briefly, cells were inoculated at a density of 5 x 105/ml into 4,000 ml of M-M medium and cultured in a spinner flask with constant stirring at 45 rpm for about 5 days.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Purification and characterization of a 59-kilodalton protein that specifically binds to NF-kappa B-binding motifs of the defense protein genes of Sarcophaga peregrina (the flesh fly).

Kobayashi¹,

Matsui²,

Kubo³

et al. 1993

Mol. Cell. Biol.

View full text Add to dashboard Cite

Various Sarcophaga peregrina (flesh fly) defense protein genes were shown to be activated when NIH-Sape-4 cells were cultured with bacterial lipopolysaccharides or 0-1,3-glucan. The 5' upstream regions of the defense protein genes were found to have common motifs showing similarity to the mammalian NF-KB-binding consensus sequence. A protein with affinity to the NF-KB-binding motif of the Sarcophaga lectin promoter was identified and purified to near homogeneity. This 59-kDa protein also bound to the NF-KB-binding motifs of other defense protein genes, e.g., sarcotoxin I and sarcotoxin II genes. This protein was found in both the cytoplasmic and the nuclear fractions of the cells, and it appeared to migrate from the cytoplasm to the nucleus on treatment of the cells with lipopolysaccharides. This 59-kDa protein is probably a transcriptional regulator of the genes for defense proteins of S. peregnna.

show abstract

Synthesis of Sarcophaga Lectin and Sarcotoxins in Nih-Sape-4, an Embryonic Cell Line of Sarcophaga Peregrina

Cited by 4 publications

References 10 publications

Immunity proteins from mosquito cell lines include three defensin A isoforms from Aedesaegypti and a defensin D from Aedesalbopictus

Immunity proteins from mosquito cell lines include three defensin A isoforms from Aedesaegypti and a defensin D from Aedesalbopictus

In vitro CULTURE OF FISH LOUSE (Lepeoptheirus salmonis) IN ATLANTIC SALMON (Salmo salar L. ) HOST CELL LINE

Purification and characterization of a 59-kilodalton protein that specifically binds to NF-kappa B-binding motifs of the defense protein genes of Sarcophaga peregrina (the flesh fly).

Contact Info

Product

Resources

About