Synthesis of Retinoic Acid by Rat Ovarian Cells That Express Cellular Retinoic Acid-Binding Protein-II1

Zheng, Wen; Bucco, R A; Sierra-Rievera, Elaine; Osteen, Kevin G.; Melner, Michael H.; Ong, David E.

doi:10.1095/biolreprod60.1.110

Cited by 38 publications

(29 citation statements)

References 31 publications

(28 reference statements)

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“…Consistent with that idea, we have now observed coincident appearance of the ability to synthesize RA and expression of CRABP(II) in certain cell types in addition to the uterine epithelium: the developing granulosa cells of the ovarian follicle prior to ovulation and in stromal cells of the uterus undergoing the process of decidualization (2,8,9). Human mammary ductal epithelium also has the ability to synthesize RA, and those cells express CRABP(II) as well (10).…”

supporting

confidence: 61%

Cellular Retinoic Acid-binding Protein II Gene Expression Is Directly Induced by Estrogen, but Not Retinoic Acid, in Rat Uterus

Ong

2003

Journal of Biological Chemistry

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It has been suggested that cellular retinoic acid-binding protein (II) (CRABP(II)) may have a role in the movement of retinoic acid (RA) to its nuclear receptors, thereby enhancing the action of RA in the cells in which it is expressed. RA has also been shown to increase expression of CRABP(II). Previous work from our laboratory has shown that 17␤-estradiol (E 2 ) administration to prepubertal female rats leads to acquisition of the ability of the lining epithelium to synthesize RA as well as to express CRABP(II). To determine whether this appearance of CRABP(II) was dependent on the production of RA, both E 2 and RA were administered to ovariectomized rats. E 2 administration induced expression of the CRABP(II) gene in the uterus within 4 h, and this induction was not inhibited by prior administration of puromycin, indicating that the induction was direct. In contrast, RA caused no change in CRABP(II) message level, even at times as late as 48 h after administration. Isolation and analysis of 4.5 kb of the 5-flanking region of the gene revealed no apparent E 2 -response element. Using this portion of the gene to drive expression of the luciferase gene in transfected cells allowed identification of a region containing an imperfect estrogen-response element and estrogen-response element half-site, necessary for E 2 -driven induction. A possible Sp1 binding site in the 5-flanking region of the CRABP(II) gene was also required for this induction. The ability of E 2 to induce expression of CRABP(II) suggests that it can enhance the activity of RA, directly affecting expression of retinoid-responsive genes.

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supporting

confidence: 61%

Cellular Retinoic Acid-binding Protein II Gene Expression Is Directly Induced by Estrogen, but Not Retinoic Acid, in Rat Uterus

Ong

2003

Journal of Biological Chemistry

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“…Cellular retinoic acid binding protein (CRABP) protects against RA excess and limits the transcriptional potential of RA (Morris-Kay and Ward, 1999). Although CRABP has not been investigated in bovine, the existence of CRABP II in rats is restricted to granulosa cells from mature follicles and luteal cells (Bucco et al, 1995;Wardlaw et al, 1997;Zheng et al, 1999). Therefore, protection against RA in cumulus-granulosa cells surrounding immature bovine oocytes, such as those used in the present experiments, could be reduced.…”

Section: Discussionmentioning

confidence: 96%

9‐cis‐retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF‐I expression in cumulus‐granulosa cells

Gómez

Royo

Duque

et al. 2003

Molecular Reproduction Devel

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The isomer 9-cis of retinoic acid (9-cis-RA) exerts a beneficial effect on bovine in vitro development when added to in vitro maturation (IVM) culture. In the present work, 9-cis-RA 5 nM was found to be stimulatory as opposed to 500 nM (toxic). Cumulus-oocyte complexes (COCs) were treated with the found physiological dose 9-cis-RA 5 nM, and the next determinations performed: (1) relative expression of midkine (MK) and IGF-I, by reverse transcriptasepolymerase chain reaction (RT-PCR), in cumulusgranulosa cells detached from oocytes; (2) cytoplasmic granular migration, by labeling of oocytes with fluoroscein isothiocyanate lectins; and (3) in vitro survival of blastocysts after vitrification and warming. Gene expression of MK was enhanced by 9-cis-RA, but not by 1% ethanol (vehicle). However, we did not detect IGF-I expression, both in dependence on or in the absence of 9-cis-RA acting on cumulus-granulosa cells. The ability of vitrified blastocysts to survive in vitro was not improved by 9-cis-RA. Nevertheless, since only blastocysts obtained from oocytes matured with serum survived, more factors should be considered when evaluating cryopreservation survival. The complete granular migration observed in oocytes matured with 9-cis-RA anticipates the gain in developmental competence of the oocyte, being MK probably involved in this beneficial effect. Mol. Reprod.

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“…After entry into the cell, ROH can be metabolized to RA, which can then be bound by specific CRABPs. As previously demonstrated in rats (Bucco et al 1995, Wardlaw et al 1997, Zheng et al 1999, intracellular proteins that regulate RA availability may not be expressed in immature follicles; as a result the availability of RA in the follicle could have gone unchecked. On the contrary, under in vitro conditions, by providing a carefully calculated dose, it is possible to ensure that an optimum concentration of RA is made available to the COCs during in vitro culture.…”

Section: Embryos Transferred Pregnancy (%)mentioning

confidence: 94%

“…In the pig, detection of CRABP I is cycle stage-dependent, and luteal cells in dioestrus, together with oviduct and uterus in oestrus, contain the protein (Schweigert & Siegling 2001). Rats have CRABP II in granulosa cells from mature follicles and luteal cells (Bucco et al 1995, Wardlaw et al 1997, Zheng et al 1999.…”

Section: Introductionmentioning

confidence: 99%

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

Hidalgo¹,

Díez²,

Duque³

et al. 2005

Reproduction

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Retinoids have been shown to enhance developmental competence of the oocyte in cattle, sheep and pigs. In this study we investigated whether exogenous retinol stimulates the bovine oocyte during its intrafollicular growth and the time limits of exposure to exogenous retinol. In addition, we also determined the efficiency of ovum pick-up techniques in combination with retinol treatment and the viability of embryos after transfer to recipients. In Experiment 1, heifers were injected with retinol or vehicle, and concentrations of retinol in the blood were analysed on Day 0 (prior to injection), Day 1 and, together with follicular fluid, Day 4. Blood retinol increased by Day 1 and cleared on Day 4, but retinol remained higher within the follicle. In Experiment 2, oocyte donors were injected weekly with retinol or vehicle four times during a twice-per-week cycle of eight recovery sessions (starting 4 days before the first session), followed by a second eight-session cycle without treatment. Oocytes recovered were fertilized and culturedin vitro.Retinol treatment yielded higher numbers of low-quality oocytes throughout, although retinol measured during cycles did not change. Total oocytes, and morulae and blastocyst rates, increased during the first five sessions following treatment with retinol. As previously shown with oocytes from slaughterhouse ovaries, retinoic acid stimulated blastocyst development. Following transfer to recipients, blastocysts from oocytes exposed to retinol were unable to establish pregnancy. Our study confirms the existence of an effect of retinol on the intrafollicular oocyte in the cow and provides evidence regarding the teratogenic effect of retinol.

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Synthesis of Retinoic Acid by Rat Ovarian Cells That Express Cellular Retinoic Acid-Binding Protein-II1

Cited by 38 publications

References 31 publications

Cellular Retinoic Acid-binding Protein II Gene Expression Is Directly Induced by Estrogen, but Not Retinoic Acid, in Rat Uterus

Cellular Retinoic Acid-binding Protein II Gene Expression Is Directly Induced by Estrogen, but Not Retinoic Acid, in Rat Uterus

9‐cis‐retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF‐I expression in cumulus‐granulosa cells

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

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