Synthesis of Reovirus Oligo Adenylic Acid In Vivo and In Vitro

Abstract

mM), pyruvate kinase (25 tLg/ml), and actinomycin D (10 ,g/ml) were added to all reaction mixtures. (ii) Chymotrypsin (100 sg/ml) was added to the reaction 740

Structure and function of the reovirus genome

“…Furthermore, the synthesis of oligoadenylates may then be an expression of a partial activity of the transcriptase in the process of being inactivated: its Km for adenosine triphosphate may then greatly exceed that for other nucleoside triphosphates, which would result in its catalyzing the template-independent polymerization of adenosine triphosphate to short oligoadenylates. Indeed, Silverstein et al (95) found that oligoadenylates are formed within nascent virus particles during the final stages of morphogenesis and suggested that the oligoadenylate polymerase activity may be an alternative activity of the viral transcriptase regulated by outer capsid proteins. Furthermore, Johnson et al (36) found that late temperature-sensitive mutants of reovirus that synthesize noninfectious and therefore presumably defective particles at nonpermissive temperatures do not synthesize oligoadenylates at nonpermissive temperatures, which again supports the conclusion that they are synthesized during the final stages of virus maturation.…”

Reovirus-Specific Enzyme(s) Associated with Subviral Particles Responds In Vitro to Polyribocytidylate to Yield Double-Stranded Polyribocytidylate·Polyriboguanylate

“…Five other enzymatic activities have been detected in the virus particle (36). Only one, the polyadenylic acid [poly(A)] polymerase (34,38), is active when the virus is complete. The remaining four are manifest only in viral cores or subviral particles.…”