Synthesis of Quercetin Functionalized Silver Nanoparticles and Their Application for the Colorimetric Detection of L‐Cysteine in Biologically Complex Fluids

Abstract

In the present manuscript authors report an eco‐friendly, simple, and cost‐effective one‐pot synthesis of quercetin functionalized silver nanoparticles (Q‐AgNPs). The synthesized Q‐AgNPs exhibited surface plasmonic resonance absorption band at 418 nm which confirmed the synthesis. The synthesized Q‐AgNPs were further characterized by X‐ray diffraction which confirmed the crystalline structure of Q‐AgNPs. FTIR analysis of Q‐AgNPs was performed to find out the functional groups present on Q‐AgNPs. The distribution, size, and morphology of Q‐AgNPs were determined through scanning electron microscopy (SEM), transmission electron microscopy (TEM), and dynamic light scattering (DLS) analysis. SEM, TEM, and DLS analysis revealed that synthesised nanoparticles were spherical in morphology with an average size of 40 nm. Furthermore, the synthesised Q‐AgNPs were observed to selectively sense cysteine in water, urine, and foetal bovine serum with detection limit of 21.1 nM, 86 nM, and 230 nM respectively. The SEM, TEM, and DLS analysis clearly indicated the aggregation of Q‐AgNPs in the presence of l‐cysteine particles as compared to the control Q‐AgNPs (without l‐cysteine). The findings revealed that synthesized Q‐AgNPs can be utilized to detect low concentration of cysteine in water and biological complex fluids without any requirement of sophisticated instrumentation.

Synthesis of Quercetin-Loaded Silver Nanoparticles and Assessing Their Anti-Bacterial Potential

“…In the context of medication compatibility testing, FTIR analysis of Qu-AgNPs was performed, as depicted in Figure 2. It was observed that all spectral groups exhibited consistent values, affirming the authenticity of the drug sample and confirming the absence of any discernible impurities [29].…”

Eugenia uniflora L. Silver and Gold Nanoparticle Synthesis, Characterization, and Evaluation of the Photoreduction Process in Antimicrobial Activities

“…E. uniflora L. phytochemicals, such as saponin, tannins, phenolic compounds, and flavonoids [ 51 , 52 ], interact with AgNO 3 reduced to elemental silver or gold. Biosynthesis probably involves quercetin [ 53 ], carotenoids, and luteolin [ 54 ]. The presence of silver nanoparticles is confirmed in Figure 2 b.…”

A self-cascade system based on Ag nanoparticle/single-walled carbon nanotube nanocomposites as an enzyme mimic for ultrasensitive detection of l-cysteine

“…9 Considering the aforementioned properties, the detection of l -cysteine is of great significance for human health and food quality assurance. To date, researchers have developed a multitude of analytical techniques for l -cysteine detection, for instance, chromatography, 10 electrochemistry, 11 colorimetry, 12 and so on. However, despite their superior performance in detecting l -cysteine, their applications are limited by the unavailability of large-scale equipment, complicated sample pretreatment, and laborious operation processes.…”